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Establishment of Pre-Harvest Residue Limit of Fungicides Pyrimethanil and Trifloxystrobin during Cultivation of Persimmon

단감 재배기간 중 살균제 pyrimethanil과 trifloxystrobin의 생산단계 잔류허용기준 설정

  • Lee, Dong-Yeol (Division of Applied Life Science (BK21 Program) & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Yeong-Jin (Division of Applied Life Science (BK21 Program) & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, So-Jung (Division of Applied Life Science (BK21 Program) & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Cho, Kyu-Song (Division of Applied Life Science (BK21 Program) & Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Sang-Gon (Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Park, Min-Ho (Gyeongnam Provincial Office, National Agricultural Products Quality Management Service) ;
  • Kang, Kyu-Young (Division of Applied Life Science (BK21 Program) & Institute of Agriculture and Life Science, Gyeongsang National University)
  • 이동열 (경상대학교 응용생명과학부(BK21 농생명산업 글로벌 인재 육성 사업단) & 경상대학교 농업생명과학연구원) ;
  • 김영진 (경상대학교 응용생명과학부(BK21 농생명산업 글로벌 인재 육성 사업단) & 경상대학교 농업생명과학연구원) ;
  • 이소정 (경상대학교 응용생명과학부(BK21 농생명산업 글로벌 인재 육성 사업단) & 경상대학교 농업생명과학연구원) ;
  • 조규성 (경상대학교 응용생명과학부(BK21 농생명산업 글로벌 인재 육성 사업단) & 경상대학교 농업생명과학연구원) ;
  • 김상곤 (경상대학교 식물생명공학연구소) ;
  • 박민호 (농산물품질관리원 경남지원) ;
  • 강규영 (경상대학교 응용생명과학부(BK21 농생명산업 글로벌 인재 육성 사업단) & 경상대학교 농업생명과학연구원)
  • Received : 2012.03.12
  • Accepted : 2012.03.26
  • Published : 2012.03.31

Abstract

BACKGROUND: This study was carried out to establish pre-harvest residue limit of fungicides pyrimethanil and trifloxystrobin in persimmon, based on dissipation and biological half-lives of two fungicides residue. METHODS AND RESULTS: Both pyrimethanil and trifloxystrobin were extracted with acetonitrile, clean-up with $NH_2$ SPE cartridge and residue were analyzed by HPLC/DAD. Limit of Detection was 0.01 mg/kg. Average recovery were $81{\pm}1.62%$, $98{\pm}1.58%$ of pyrimethanil, and $91{\pm}2.94%$, $98{\pm}1.25%$ of trifloxystrobin at fortification levels at 0.1 and 0.5 mg/kg, respectively. CONCLUSION: The biological half-lives of pyrimethanil were 15.6 and 11.6 days at sprayed with recommended and double dosage, respectively. The biological half-lives of trifloxystrobin were 10.4 and 10.3 days at sprayed with recommended and double dosage, respectively. The pre-harvest residue limit of pyrimethanil and trifloxystrobin were recommended as 2.69 and 0.83 mg/kg for 10 days before harvest, respectively.

본 연구는 단감 재배 중 사용되는 살균제인 pyrimethanil과 trifloxystrobin의 생산단계 잔류허용기준 설정을 통하여 안전한 단감 생산에 기여하고자 수행되었다. 먼저 pyrimethanil과 trifloxystrobin을 살포하고, 살포 후 0, 1, 2, 3, 5, 7, 10, 12, 15, 18일에 단감 시료를 채취하여 각각의 농약을 분석하고 생물학적 반감기를 산출한 다음 생산단계 잔류허용기준(PHRL; Pre-Harvest Residue Limit)을 설정하였다. 단감 중 pyrimethanil과 trifloxystrobin은 Acetonitrile을 이용하여 추출하고 $NH_2$ cartridge와 PSA를 이용하여 정제하여 HPLC/DAD를 이용하여 분석하였다. 두 농약에서 검출한계는 모두 0.01 mg/kg이었다. Pyrimethanil의 회수율은 0.1과 0.5 mg/kg 두 수준에서 각각 $81{\pm}1.62%$, $98{\pm}1.58%$ 이었으며, trifloxystrobin의 회수율은 0.1과 0.5 mg/kg 두 수준에서 각각 $91{\pm}2.94%$, $98{\pm}1.25%$이었다. 단감에서 pyrimethanil의 생물학적 반감기는 기준량 살포 시 15.6일, 배량 살포 시 11.6일이었고, trifloxystrobin의 생물학적 반감기는 기준량 살포 시 10.4일, 배량 살포 시 10.3일이었다. 잔류회귀 감소식을 이용한 생산단계 잔류허용기준은 pyrimethanil과 trifloxystrobin은 각각 수확 10일 전 2.69 mg/kg과 0.83 mg/kg으로 제안하였다.

Keywords

References

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