Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Anti-inflammatory Effects by Arctium lappa L. Root Extracts through the Regulation of ICAM-1 and Nitric Oxide

우엉뿌리추출물이 ICAM-1과 NO조절에 미치는 항염증효과

  • Kim, Ye-Jin (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Kang, Se-Chan (Department of Natural Medicine Resources, Semyung University) ;
  • NamKoong, Seung (Department of Physical Therapy, Kangwon National University) ;
  • Choung, Myoung-Gun (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Sohn, Eun-Hwa (Department of Herbal Medicine Resource, Kangwon National University)
  • 김예진 (강원대학교 생약자원개발학과) ;
  • 강세찬 (세명대학교 자연약재과학과) ;
  • 남궁승 (강원대학교 물리치료학과) ;
  • 정명근 (강원대학교 생약자원개발학과) ;
  • 손은화 (강원대학교 생약자원개발학과)
  • Received : 2011.08.29
  • Accepted : 2011.11.16
  • Published : 2012.02.29
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Abstract

Atopic dermatitis is a chronic, allergic inflammatory skin disease that is accompanied by pruritic chronic eczema and markedly increased levels of inflammatory cells in endothelial cells. Arctium lappa L. is a popular edible vegetable cultivated in Asia. This study examined the effect of butanol extracts of A. lappa (ALBE) on the expression of adhesion molecule, ICAM-1 and the production of NO-iNOS induced by TNF-alpha in A549 endothelial cells. We also studied the effects of ALBE on the proliferation of keratinocyte. We observed significant inhibition of NO-iNOS production in dose-dependant manners and ALBE at $100\;{\mu}g$/mL suppressed the expression of ICAM-1 in TNF-${\alpha}$-induced A549 cells. In addition, the treatment of ALBE for 48 hrs increased the proliferation of HaCaT keratinocytes. These results support that ALBE has an anti-inflammatory effects for the treatment of atopic dermatitis.

본 연구는 우엉추출물의 항염증효과를 측정하였다. 인간 유래 폐상피세포 A549를 이용하여 염증반응을 유발하고 유지시키는데 중요한 역할을 나타낸다고 알려진 ICAM-1의 억제적 조절 효과를 평가하였으며, 염증매개인자로 알려진 NO와 이의 생성에 영향을 미치는 iNOS의 발현조절에서도 억제효과가 있음을 확인하였다. 또한, 우엉추출물이 인간각질형성세포주의 세포증식에 효과가 있음을 나타내었다. 본 연구에서 사용되는 폐상피세포는 호흡기질환의 천식 등의 알러지 및 만성염증연구에 주로 활용되고 있으며, 각질형성세포는 아토피 피부염의 만성 염증반응에서 주요하게 확인하는 요소이다. 따라서 본 연구결과에서 나타난 우엉추출물의 항염증 효과는 다양한 염증반응에서도 특히, 알러지 및 아토피 피부염 질환을 더욱 악화시키는 만성 염증에 있어서 우엉추출물이 유용한 소재로 사용될 수 있음을 시사한다고 할 수 있다.

Keywords

References

  1. Chen, F.A., A.B. Wu and C.Y. Chen. 2004. The influence of different treatment on the free radical scavenging activity of burdock and variations of its active components. Food Chem. 86:479-484. https://doi.org/10.1016/j.foodchem.2003.09.020
  2. Esposito, E. and S. Cuzzocrea. 2007. The role of nitric oxide synthases in lung inflammation. Curr. Opin. Investig. Drugs 8(11):899-909.
  3. Fuchigami, M., Y. Kishigami and A. Sasaki. 1990. Pectic polysaccharides in edible burdock root. J. Home Economics Japan 41:947-962.
  4. Fukamizu, R., M.H. Jang and M. Miyasaka. 2009. The role of eosinophils in allergic inflammation and intestinal immunity. Nippon Rinsho. 67(11):2088-2093.
  5. Heo, Y. and H.A. Kim. 2008. Correlation between skin prick test and enzyme-linked immunoabsorbent assay using serum for identification of subjects positive to major respiratory allergens. J. Env. Hlth. Sci. 24(5):369-373.
  6. Ishihara, K., N. Yamagishi, Y. Saito, M. Takasaki, T. Konoshima and T. Hatayama. 2006. Arctigenin from Fructus arctii is a novel suppressor of heat shock response in mammalian cells. Cell Stress Chaperones 11(2):154-161. https://doi.org/10.1379/CSC-148R.1
  7. Jeong, J.B., S.C. Hong, H.J. Jeong and J.S. Koo. 2011. Arctigenin induces cell cycle arrest by blocking the phosphorylation of Rb via the modulation of cell cycle regulatory proteins in human gastric cancer cells. Int. Immunopharmacol. 11(10):1573-1577. https://doi.org/10.1016/j.intimp.2011.05.016
  8. Kang, N.S. S. Pyo and E.H. Sohn. 2009. Inhibitory effects of allicin on TNF-$\alpha$-induced ICAM-1 expression is associated with catalase. J. Korean Plant Res. 22(6):552-557.
  9. Kou, X., S. Qi, W. Dai, L. Luo and Z. Yin. 2011. Arctigenin inhibits lipopolysaccharide-induced iNOS expression in RAW264.7 cells through suppressing JAK-STAT signal pathway. Int. Immunopharmacol. 11(8):1095-102. https://doi.org/10.1016/j.intimp.2011.03.005
  10. Kwon, S., R.L. Newcomb and S.C. George. 2001. Mechanisms of synergistic cytokine-induced nitric oxide production in human alveolar epithelial cells. Nitric Oxide 5(6):534-546. https://doi.org/10.1006/niox.2001.0387
  11. Lebovidge, J.S., H. Strauch, L.A. Kalish and L.C. Schneider. 2009. Assessment of psychological distress among children and adolescents with food allergy. J. Allergy Clin. Immunol. 124(6):1282-1288. https://doi.org/10.1016/j.jaci.2009.08.045
  12. Lee, M.Y., S.L. Shin, S.H. Park, N.R. Kim, Y.D. Chang and C.H. Lee. 2009. Development of optimal cultivation conditions and analysis of antioxidant activities of Arctium lappa sprout vegetables. J. Korean Plant Res. 22(4): 281-363 (in Korean).
  13. Lin, C.C., J.M. Lu, J.J. Yang, S.C. Chuang and T. Ujiie. 1996. Anti-inflammatory and radical scavenge effects of Arctium lappa. American J. Chin. Med. 24:127-137. https://doi.org/10.1142/S0192415X96000177
  14. Lin, S.C., C.H. Lin, C.C. Lin, Y.H. Lin, C.F. Chen, J.C. Chen and L.Y. Wang. 2002. Hepatoprotective effects of Arctium lappa L. on liver injuries induced by chronic ethanol consumption and potentiated by carbon tetrachloride tetrachloride. J. Biomed. Sci. 9:401-409.
  15. Maruta, Y., J. Kawabata and R. Niki. 1995. Antioxidative caffeoylquinic acid derivatives in the roots of burdock (Arctium lappa L.). J. Agric. Food Chem. 43:2592-2595. https://doi.org/10.1021/jf00058a007
  16. Matsuzaki, Y., M. Koyama, T. Hitomi, T. Yokota, M. Kawanaka, A. Nishikawa, D. Germain and T. Sakai. 2008. Arctiin induces cell growth inhibition through the down-regulation of cyclin D1 expression. Oncol. Rep. 19(3):721-727.
  17. Morita KY, Y. Nishijima and T. Kada. 1985. Chemical nature of a desmutagenic factor from burdock (Arctium lappa L.). Agric. Biol. Chem. 49:925-932. https://doi.org/10.1271/bbb1961.49.925
  18. Park, S.Y., S.S. Hong, X. Han, J.S. Hwang, D. Lee, J.S. Ro and B.Y. Hwang. 2007. Lignans from Arctium lappa and their inhibition of LPS-induced nitric oxide production. Chem. Pharm. Bull. 55:150-152. https://doi.org/10.1248/cpb.55.150
  19. Schaefer, L. and K. Kragballe. 1991. Abnormalities in epidermal lipid metabolism in patients with atopic dermatitis. J. Invest. Dermatol. 96:10-15. https://doi.org/10.1111/1523-1747.ep12514648
  20. Sohn, E.H., S.A. Jang, H. Joo, S. Park, S.C. Kang, C.H. Lee and S.Y. Kim. 2011. Anti-allergic and anti-inflammatory effects of butanol extract from Arctium Lappa L. Clin. Mol. Allergy 9(1):4. https://doi.org/10.1186/1476-7961-9-4
  21. Strieter, R., S. Kunkel and R. Bone. 1993. Role of Tumor Necrosis Factor-Alpha in Disease States and Inflammation. Critical Care Medicine 21:S447-S463. https://doi.org/10.1097/00003246-199310001-00006
  22. Wu, J.G., J.Z. Wu, L.N. Sun, T. Han, J. Du, Q. Ye, H. Zhang and Y.G. Zhang. 2009. Ameliorative effects of arctiin from Arctium lappa on experimental glomerulonephritis in rats. Phytomedicine 16(11):1033-1041. https://doi.org/10.1016/j.phymed.2009.04.005
  23. Xu, Y. and S. Li. 2009. Blockade of ICAM-1: a novel way of vasculitis treatment. Biochem. Biophys. Res. Commun. 459-461.

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