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Expression of ssrA in non-pathogen-induced adaptation in the oral cavity through signal exchange with oral pathogens

  • Kim, Sung-Ryoul (Department of Oral and Maxillofacial Surgery, School of Dentistry, Dental Science Research Institute, 2nd Stage of Brain Korea 21, Chonnam National University) ;
  • Kwak, Jae-Woo (Department of Oral and Maxillofacial Surgery, School of Dentistry, Dental Science Research Institute, 2nd Stage of Brain Korea 21, Chonnam National University) ;
  • Lee, Sung-Ka (Department of Oral and Maxillofacial Surgery, School of Dentistry, Dental Science Research Institute, 2nd Stage of Brain Korea 21, Chonnam National University) ;
  • Jung, Seung-Gon (Department of Oral and Maxillofacial Surgery, School of Dentistry, Dental Science Research Institute, 2nd Stage of Brain Korea 21, Chonnam National University) ;
  • Han, Man-Seung (Department of Oral and Maxillofacial Surgery, School of Dentistry, Dental Science Research Institute, 2nd Stage of Brain Korea 21, Chonnam National University) ;
  • Kim, Bang-Sin (Department of Oral and Maxillofacial Surgery, School of Dentistry, Dental Science Research Institute, 2nd Stage of Brain Korea 21, Chonnam National University) ;
  • Kook, Min-Suk (Department of Oral and Maxillofacial Surgery, School of Dentistry, Dental Science Research Institute, 2nd Stage of Brain Korea 21, Chonnam National University) ;
  • Oh, Hee-Kyun (Department of Oral and Maxillofacial Surgery, School of Dentistry, Dental Science Research Institute, 2nd Stage of Brain Korea 21, Chonnam National University) ;
  • Park, Hong-Ju (Department of Oral and Maxillofacial Surgery, School of Dentistry, Dental Science Research Institute, 2nd Stage of Brain Korea 21, Chonnam National University)
  • Received : 2011.08.25
  • Accepted : 2012.01.20
  • Published : 2012.02.29

Abstract

Introduction: This study was conducted to evaluate ssrA expression resulting from adaptation of Escherichia coli (E. coli) to oral pathogens through signal exchange. Materials and Methods: Human cell lines Hep2 and HT29, wild-type E. coli (WT K-12), ssrA knock-out E. coli (${\Delta}K$-12), and Scleropages aureus (S. aureus) were used. A single culture consisting of Hep2, HT29, WT K-12, and ${\Delta}K$-12, and mixed cultures consisting of Hep2 and WT K-12, Hep2 and ${\Delta}K$-12, WT K-12 and S. aureus, ${\Delta}K$-12 and S. aureus, and Hep2, WT K-12, and S. aureus were prepared. For HT29, a mixed culture was prepared with WT K-12 and with WT K-12 and S. aureus. Total RNA was extracted from each culture with the resulting expression of ssrA, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-${\kappa}B$), and p53 was evaluated by Reverse transcription polymerase chain reaction (RT-PCR). Results: The expression of ssrA in a single culture of WT K-12 was lower than that observed in the mixed culture of WT K-12 with S. aureus. Greater ssrA expression was observed in the mixed culture of WT K-12 with Hep2 than in the single culture of WT K-12. The expression of NF-${\kappa}B$ was higher in the mixed culture of Hep2 with ${\Delta}K$-12 than that in the mixed culture of Hep2 with WT K-12, and was lowest in the single culture of Hep2. The expression of ssrA was higher in the mixed culture of WT K-12 with Hep2 and S. aureus than in the mixed culture of WT K-12 with Hep2. Conclusion: These results suggest that ssrA plays an important role in the mechanism of E. coli adaptation to a new environment.

Keywords

References

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