Asian Pacific Journal of Cancer Prevention

  • 제13권12호
  • /
  • Pages.6429-6433
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  • 2012
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Effects of Sodium Valproate on the Growth of Human Ovarian Cancer Cell Line HO8910

  • Yan, Hong-Chao (Department of Obstetrics and Gynecology, the Affiliated Hospital of Xuzhou Medical College) ;
  • Zhang, Jie (Department of Obstetrics and Gynecology, the Affiliated Hospital of Xuzhou Medical College)
  • 발행 : 2012.12.31
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초록

To explore a possible new treatment for human ovarian cancer, we studied the effects of sodium valproate on the growth of the HO8910 human cell line. HO8910 cells were cultured in vitro and treated with different concentrations of sodium valproate. Cell proliferation, cell cycling, and apoptosis were measured by flow cytometry, cell morphology under a microscope, and expression levels of WWOX and P27 by Western blotting and RT-PCR. Tumor xenografts were established to determine in vivo effects of sodium valproate. Our results showed that cell proliferation was decreased with increasing concentration of sodium valproate, with features of cytoplasmic retraction and floating cells. Moreover, cell cycle analysis revealed a higher apoptosis rate and $G_0/G_1$ phase in the sodium valproate experimental group than in the control group. In addition, protein expression levels of WWOX and P27 were elevated. Importantly, sodium valproate decreased in vivo xenograft tumor burden and up-regulated WWOX and P27 expression in nude mice. In conclusion, sodium valproate might play a role in inhibition and control of ovarian cancer cell line HO8910 by inhibiting cell proliferation, interfering with the cell cycle and promoting apoptosis, so that it may be effective in the clinical treatment of ovarian cancer.

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참고문헌

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피인용 문헌

  1. WW domain-containing oxidoreductase’s role in myriad cancers: Clinical significance and future implications vol.239, pp.3, 2014, https://doi.org/10.1177/1535370213519213
  2. Ectopic expression of the WWOX gene suppresses stemness of human ovarian cancer stem cells pp.1792-1082, 2015, https://doi.org/10.3892/ol.2015.2971
  3. Proliferation inhibition of cisplatin-resistant ovarian cancer cells using drugs screened by integrating a metabolic model and transcriptomic data vol.50, pp.6, 2017, https://doi.org/10.1111/cpr.12370
  4. Evaluation of the mechanism of epithelial-mesenchymal transition in human ovarian cancer stem cells transfected with a WW domain-containing oxidoreductase gene vol.8, pp.1, 2014, https://doi.org/10.3892/ol.2014.2063