Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Association Between p16, hMLH1 and E-cadherin Promoter Hypermethylation and Intake of Local Hot Salted Tea and Sun-dried Foods in Kashmiris with Gastric Tumors

  • Mir, Manzoor R. (Division of Veterinary Biochemistry, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir) ;
  • Shabir, Nadeem (Division of Veterinary Biochemistry, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir) ;
  • Wani, Khursheed A. (Department of General Surgery, Sher-I-Kashmir Institute of Medical Sciences) ;
  • Shaffi, Sheikh (Division of Veterinary Biochemistry, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir) ;
  • Hussain, Ishraq (Division of Veterinary Biochemistry, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir) ;
  • Banday, Manzoor A. (Department of Medical Oncology, Sher-I-Kashmir Institute of Medical Sciences) ;
  • Chikan, Naveed A. (Department of Medical Biotechnology, Vellore Institute of Technology) ;
  • Bilal, S. (Division of Veterinary Biochemistry, Faculty of Veterinary Science and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir) ;
  • Aejaz, Sheikh (Department of Medical Oncology, Sher-I-Kashmir Institute of Medical Sciences)
  • Published : 2012.01.31
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Abstract

The aim of this study was to evaluate the methylation status of three important cancer related genes viz. p16, E-cadherin and hMLH1 promoters and to associate the findings with specific dietary habits in Kashmiris, a culturally distinct population in India, with gastric cancer. The study subjects were divided into three age groups viz. 0-30yrs ($1^{st}$), 31-60yrs ($2^{nd}$) and 61-90yrs ($3^{rd}$). A highly significant association between the intake of local hot salted tea in $2^{nd}$ (p=0.001) and $3^{rd}$ (p=0.009) age groups was observed with the promoter hypermethylation of E cadherin. Again a highly significant association between the aberrant methylation of hMLH1 (p=0.000) and p16 (p=0.000) promoters and the intake of local hot salted tea was observed in the $2^{nd}$ age group of gastric cancer patients. The intake of sun-dried food was also significantly associated with the promoter hypermethylation of E cadherin (p=0.003) and p16 (p=0.015) genes in $3^{rd}$ age group. The results of the present study suggest a close association between the aberrant methylation of p16, E-cadherin and hMLH1 promoters and the intake of local hot salted tea and sun-dried foods in Kashmiri population.

Keywords

References

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