DOI QR코드

DOI QR Code

Apoptin Induces Apoptosis in Human Bladder Cancer EJ and BIU-87 Cells

  • Zhan, Hui (Department of Urology, the Second Affiliated Hospital of Kunming Medical University) ;
  • Wang, Jian-Song (Department of Urology, the Second Affiliated Hospital of Kunming Medical University) ;
  • Wang, Hai-Feng (Department of Urology, the Second Affiliated Hospital of Kunming Medical University) ;
  • Zuo, Yi-Gang (Department of Urology, the Second Affiliated Hospital of Kunming Medical University) ;
  • Wang, Chun-Hui (Department of Urology, the Second Affiliated Hospital of Kunming Medical University) ;
  • Ding, Ming-Xia (Department of Urology, the Second Affiliated Hospital of Kunming Medical University)
  • Published : 2012.01.31

Abstract

Objective: To investigate whether apoptin is a apoptosis-inducing protein with a potential for bladder cancer therapy. Methods: We constructed a PCDNA3/Apoptin eukaryotic expression vector, and transfected this vector into bladder cancer cell lines BIU-87 and EJ, then observed the results by RT-PCR, transmission electron microscopy, MTT assay and the flow cytometry (TUNEL method). Results: PCDNA3/Apoptin successfully induced a high level apoptosis in both bladder cancer cell lines, compared with the controls (p<0.05). Conclusions: Apoptin can induce high level apoptosis in human bladder cancer EJ and BIU-87 cells, which suggests a potential for human bladder cancer therapy.

References

  1. Backendorf C, Visser AE, de Boer AG, et al (2008). Apoptin: therapeutic potential of an early sensor of carcinogenic transformation. Annu Rev Pharmacol Toxicol, 48, 143-69. https://doi.org/10.1146/annurev.pharmtox.48.121806.154910
  2. Danen-van Ooschot AAAM, Zhang YH, Erkeland SJ, et al (1999). The effect of Bcl-2 on Apoptin in "normal" vs transformed human cells. Leukemia, 13, s75-7. https://doi.org/10.1038/sj.leu.2401291
  3. Danen-van Oorschot AAAM, Zhang YH, Leliveld SR, et al (2003). Importance of nuclear localization of Apoptin for tumor-specific induction of apoptosis. J Biol Chem, 278, 27729-36. https://doi.org/10.1074/jbc.M303114200
  4. Danen-van Ooschot AAAM, Voskamp P, Seelen MCMJ, et al (2004). Human death effector domain-associated factor interacts with the viral apoptosis agonist apoptin and exerts tumor- preferential cell killing. Cell Death Differ, 11, 564-73. https://doi.org/10.1038/sj.cdd.4401391
  5. Gu FL (2003). Changing constituents of genitourinary cancer in recent 50 years in Beijing. Chin Med J, 116, 1391-3.
  6. Han SX, Ma JL, Lu Y, et al (2008). [SP-TAT-Apoptin induces G1 arrest in HepG2 cells]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 24, 864-6.
  7. Han SX, Zhao J, Ma JL, et al (2010). The effect of the fused gene of SP-TAT-Apoptin transfected by lentivirus on HepG2 cells. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 26, 310-2.
  8. Han SX, Zhu Q, Ma JL, et al (2011). Apoptin sensitizes radiationinduced cell death via classic mitochondrial, caspase and p53-dependent signaling in HepG2 cells. Mol Med Report, 4, 59-63.
  9. Leliveld SR, Dame RT, Rohn JL, et al (2004). Apoptin's functional N- and C-termini independently bind DNA. FEBS Lett, 557, 155-8. https://doi.org/10.1016/S0014-5793(03)01465-0
  10. Noteborn MHM (2004). Chicken anemia virus induced apoptosis: underlying molecular mechanisms. Vet Microbiol, 98, 89-94. https://doi.org/10.1016/j.vetmic.2003.10.003
  11. Noteborn MHM (2005). Apoptin acts as a tumor-specific killer: potentials for an anti-tumor therapy. Cell Mol Biol, 51, 49-60.
  12. Poon IK, Oro CD Iasm M, et al (2005). Apoptin nuclear accumulation is modulated by a CRM1-recognized nuclear export signal that is active in normal but not in tumor cells. Cancer Res, 65, 7059-64. https://doi.org/10.1158/0008-5472.CAN-05-1370
  13. Pruthi RS, Baldwin N, Bhalani V, et al (2008). Conservative management of low risk superficial bladder tumors. J Urol, 179, 87-90.
  14. Rohn JL, Zhang YH, Aalbers RIJM, et al (2002). A tumorspecific kinase activity regulates the viral death protein apoptin. J Biol Chem, 277, 50820-70. https://doi.org/10.1074/jbc.M208557200
  15. Rohn JL, Noteborn MHM(2004). The viral death effector Apoptin reveals tumor-specific processes. Apoptosis, 9, 315-22. https://doi.org/10.1023/B:APPT.0000025808.48885.9c
  16. Schoop RA, Kooistra K, Baatenburg De Jong RJ, et al (2004). Bcl-xL inhibits p53- but not apoptin-induced apoptosis in head and neck squamous cell carcinoma cell line. Int J Cancer, 109, 38-42. https://doi.org/10.1002/ijc.11675
  17. Schoop RA, Baatenburg de Jong RJ, Noteborn MH (2008). Apoptin induces apoptosis in an oral cancer mouse model. Cancer Biol Ther, 7, 1368-73. https://doi.org/10.4161/cbt.7.9.6419
  18. Schulze M, Stotz N, Rassweiler J (2007). Retrospective analysis of transurethral resection, second-look resection, and longterm chemo-metaphylaxis for superficial bladder cancer: indications and efficacy of a differentiated approach. J Endourol, 21, 1533-41. https://doi.org/10.1089/end.2007.9866
  19. Teodoro J G, Heilman DW, Parker AE, et al (2004). The viral protein apoptin associates with the anaphase-promoting complex to induce G2 /M arrest and apoptosis in the absence of p53. Genes Dev, 15, 1952-7.
  20. Wadia JS, Wagner MV, Ezhevsky SA, et al (2004). Apoptin/ VP3 contains a concentration-dependent nuclear localization signal (NLS), not a tumorigenic selective NLS. J Virol, 78, 6077-8. https://doi.org/10.1128/JVI.78.11.6077-6078.2004
  21. Wang C, Zhang Y (2011). Apoptin gene transfer via modified wheat histone H4 facilitates apoptosis of human ovarian cancer cells. Cancer Biother Radiopharm, 26, 121-6. https://doi.org/10.1089/cbr.2010.0858
  22. Zhuang SM, Shvarts A, van Ormondt H, et al (1995). Apoptin, a protein derived from chicken anemia virus, induces p53- independent apoptosis in human osteosarcoma cells. Cancer Res, 55, 486-9

Cited by

  1. A gyrovirus infecting a sea bird vol.160, pp.8, 2015, https://doi.org/10.1007/s00705-015-2468-1