The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Protective Effect of Myeongganbo Extract on Acetaminophen-Induced Liver Injury

명간보(明肝補) 추출물의 Acetaminophen 유도 간 손상에 대한 보호효과

  • Kim, Hong-Jun (Department of Oriental Medical Prescription, Woosuk University) ;
  • Mok, Ji-Ye (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University) ;
  • Park, Kwang-Hyun (Department of Biochemistry, Chonbuk National University Medical School) ;
  • Jeon, In-Hwa (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University) ;
  • Kim, Hyeon-Soo (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University) ;
  • Hwang, Sung-Yeoun (Department of Oriental Medical Prescription, Woosuk University) ;
  • Jang, Seon-Il (School of Alternative Medicine & Health Science, College of Alternative Medicine, Jeonju University)
  • 김홍준 (우석대학교 한의과대학 방제학교실) ;
  • 목지예 (전주대학교 대체의학대학 대체건강관리학부) ;
  • 박광현 (전북대학교 의학전문대학 생화학교실) ;
  • 전인화 (전주대학교 대체의학대학 대체건강관리학부) ;
  • 김현수 (전주대학교 대체의학대학 대체건강관리학부) ;
  • 황성연 (우석대학교 한의과대학 방제학교실) ;
  • 장선일 (전주대학교 대체의학대학 대체건강관리학부)
  • Received : 2012.02.07
  • Accepted : 2012.03.16
  • Published : 2012.03.30
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Abstract

Objective : Myeongganbo (MGB) composited with Hovenia Semen, Puerariae Radix and Dioscoreae Rhizoma is the prescription for protection of liver function. The purpose of this study was to investigate the effects of MGB extract against acetaminophen (APAP)-induced liver injury in mice. Methods : MGB extract was prepared by extracting with hot distilled water. The extract was freeze-dried following filtration through vacuum distillation system. Mice fasted for overnight were orally administrated with or without MGB extract of different doses (25-200 mg/kg/day). After 30 min, APAP was orally applied with a single dose (400 mg/kg). The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured in plasmas of mice. Glutathione (GSH), glutathione peroxidase GSH-px), cyclooxygenase-2 (COX-2) activity and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) level were investigated in liver homogenates. Liver sections were stained with haematoxylin & eosin, anti-TNF-${\alpha}$ and anti-mouse COX-2 antibodies. Results : APAP treatment remarkably increased AST and ALT activities in plasma but inhibited GSH and GSH-px levels in liver homogenates. Also, liver injury was significantly accelerated by APAP treatment. Furthermore, APAP remarkably elevated COX-2 activity and TNF-${\alpha}$ levels in liver homogenates. However, administration of MGB extract was able to counteract these effects. Histological studies provided supportive evidence for biochemical and molecular analysis Conclusions : These results suggest that MGB extract has potent hepatoprotective effect against APAP-induced liver injury, these properties may contribute to liver disease care.

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References

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