대한본초학회지 (The Korea Journal of Herbology)

  • 제27권2호
  • /
  • Pages.77-83
  • /
  • 2012
  • /
  • 1229-1765(pISSN)
  • /
  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
권호 표지
DOI QR코드

DOI QR Code

우슬 물추출물의 허혈성 뇌 손상에 대한 보호효과 연구

Effects of the water extract from Achyranthis Radix on serum-deprivation-induced apoptosis in PC12 cells and transient cerebral middle artery occlusion-induced ischemic brains of rats

  • 오태우 (동국대학교 한의과대학 본초학교실) ;
  • 박기호 (동국대학교 한의과대학 본초학교실) ;
  • 이미영 (한국한의학연구원) ;
  • 최고야 (한국한의학연구원) ;
  • 박용기 (동국대학교 한의과대학 본초학교실)
  • Oh, Tae-Woo (Department of Herbology, College of Oriental Medicine, Dongguk University) ;
  • Park, Ki-Ho (Department of Herbology, College of Oriental Medicine, Dongguk University) ;
  • Lee, Mi-Young (Aging Research Center, Korea Institute of Oriental Medicine) ;
  • Choi, Go-Ya (Aging Research Center, Korea Institute of Oriental Medicine) ;
  • Park, Yong-Ki (Department of Herbology, College of Oriental Medicine, Dongguk University)
  • 투고 : 2012.02.10
  • 심사 : 2012.03.16
  • 발행 : 2012.03.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives : This work was designed to investigate the effect of The root of Achyranthes japonica Nakai (AJN) water extract on serum deprivation reperfusion-induced apoptosis in PC-12 cells and transient middle cerebral artery occlusion (tMCAO)-induced ischemic brains of rats. Methods : Apoptosis in PC12 cells was induced by serum deprivation and reperfusion. The cells were treated with AJN water extract at doses of 0.5 and 1.0 mg/ml for 24 hr after inducing the apoptosis. Cell viability was determined by WST-1 assay. The expression of caspase-3 protein was determined by Western blot. Ischemic brains were prepared from tMCAO-induced ischemic rats after oral administration with AJN at dose of 50 and 100 mg/kg, and then brain infarction was measured by TTC staining. Results : AJN significantly increased the cell viability in apoptocic-induced PC-12 cells, and also decreased the expression of caspase-3 protein. Furthermore, the administration of AJN significantly inhibited tMCAO-induced brain infarction in rats. Conclusions : Our results suggest that AJN extract has a neuroprotective property via suppressing the apoptosis in PC12 cells and the infarction of ischemic brains.

키워드

참고문헌

  1. Park SY, Goo YD, Kim WK. Treatment of brain II, development trends and strategies for stroke treatment. Promising future business item analysis of issues(BA407). Korea Institute of Science and Technology Information. 2005:1-63.
  2. Kim ES, Park DU, Lee JE. Treatment of brain I, Industrial Market Analysis. Korea Institute of Science and Technology Information. 2006:25-55.
  3. Du C, Hu R, Csernansky CA, Hsu CY, Choi DW. Very delayed infarctoin after mild focal cerebral ischemia:a role for apoptosis? J Cereb Blood Flow Metab 1996;16:195-201. https://doi.org/10.1097/00004647-199603000-00003
  4. Li Y, Chopp M, Jiang N, Yao F, Zaloga C. Temporal profile of In Situ DNA fragmentation after transient middle cerebral artery occlusion in the rat. J Cereb Blood Flow Metab 1995;15: 389-97. https://doi.org/10.1038/jcbfm.1995.49
  5. Danno K, Horio T. Formation of UV-induced apoptosis relates to the cell cycle. Br J Dermatol. 1992;107:423-28. https://doi.org/10.1111/j.1365-2133.1982.tb00385.x
  6. Golstein P. Controlling cell death. Science. 1997; 275:1081-2. https://doi.org/10.1126/science.275.5303.1081
  7. Thompson CB. Apoptosis in the pathogenesis and treatment of disease. Science. 1995;267:1456-62. https://doi.org/10.1126/science.7878464
  8. Kerr JF, Wyllie AH, Currie AR. Apoptosis:A basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer. 1972; 26:239-57. https://doi.org/10.1038/bjc.1972.33
  9. Sanchez I, Hughes RT, Mayer BJ, Yee K, Woodett JR, Avruch J, Kyriakls JM, Zon LI. Role of SAPK/ERK kinase-1 in the stress-activated pathway regulating transcription factor c-jun. Nature. 1994;372:794-8. https://doi.org/10.1038/372794a0
  10. Williams GT. Apoptosis in the immune system. J Pathol. 1994;173:1-4. https://doi.org/10.1002/path.1711730102
  11. Camilleri B, Davi F, Feuillard J, Bourgeois C, Seilhean D, Hauw JJ, Raphael M. High expression of latent membrane protein 1 of Epstein-Barr virus and Bcl-2 oncoprotein in acquired immunodeficiency syndrome-related primary brain lymphomas. Blood. 1995;86:432-5.
  12. Behl C, Hovey L, Krajewski S, Schubert D, Reed JC. Bcl-2 prevents killing of neuronal cells by glutamate but not by amyloid beta protein. Biochem Biophys Res Comm. 1993;197:949-56. https://doi.org/10.1006/bbrc.1993.2571
  13. Wyllie AH, Kerr JF, Currie AR. Cell death:The significance of apoptosis. Int Rev Cytol. 1980;6 8:251-306. https://doi.org/10.1016/S0074-7696(08)62312-8
  14. Ma A, Guo H. Effect of radix Achyranthis bidentatae on memory and endurance. Zhong Yao Cai. 1998;21:624-6.
  15. Yang Z, Zhang B, Cai L. Study on processing conditions of wine baked radix Achyranthis bidentatae. Zhong Yao Cai. 2000;23:681-2.
  16. Wang T, Cui SY, Suo YR, Lu RH. Studies on water-soluble chemical constituents in root of Achyranthes bidentata. Zhongguo Zhong Yao Za Zhi. 2004;29:649-52.
  17. Kim JH, KI JY, Ann JY, Park HJ, Kim HJ, Kwak HB, Oh JM, Kim YK. . Inhibitory Effects of Achyranthis Bidentatae Radix on Osteoclast Differentiation and Bone Resorption. Kor J Herbology. 2010;25(1):65-74.
  18. Kim CS, Park YK. The therapeutic effect of Achyranthis Radix on the collagen-induced arthritis in mice. Kor J Herbology. 2010;25(4): 129-35.
  19. Park JS, Seong NS, Lee YJ. Comparative study on the anti-oxidative effects of Achyranthis Japonicae Radix, Achyranthis Bidentatae Radix and Cyathulae Radix. Kor J Herbology. 2007; 22(4):155-67.
  20. Shornikova AV, Casas IA, Isolauri E. Lactobacills reuteri as therapeutic agent in acute diarrhoea in young children. J. Pediatr. Gastroenterol. Nutr. 1997;24:399-404. https://doi.org/10.1097/00005176-199704000-00008
  21. Marcone MF, Jahaniaval F, Aliee H, Kakuda Y. Chemical characterization of Achyranthes bidentata seed. Food Chemistry. 2003;81:7-12. https://doi.org/10.1016/S0308-8146(02)00250-9
  22. Kim MS, SO HS, Park JS, Lee KM, Moon BS, Lee HS, Kim TY, Moon SK, Park RK. Hwansodan protects PC12 cells against serum-deprivation-induced apoptosis via a mechanism involving Ras and mitogen-activated protein (MAP) kinase pathway. General Pharmacology. 2000;34:227-35. https://doi.org/10.1016/S0306-3623(00)00065-3
  23. Ishiyama M, Tominaga H, Shiga M, Sasamoto K, Ohkura Y, Ueno K. A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet. Biol. Pharmaceut. Bull. 1996;19: 1518-20. https://doi.org/10.1248/bpb.19.1518
  24. Bederson JB, Pitts LH, Tsuji M, Nishimura MC, Davis RL, Bartkowski H. Rat middle cerebral artery occlusion: evaluation of the model and development of a neurologic examination. Stroke. 1986;17(3):472-76. https://doi.org/10.1161/01.STR.17.3.472
  25. Lo EH, Moskowitz MA, Jacobs TP. Exciting, radical, suicidal: How brain cells die after stroke. Stroke. 2005;36:189-92. https://doi.org/10.1161/01.STR.0000153069.96296.fd
  26. Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc Natl Acad Sci USA. 1976;7 3:2424-28. https://doi.org/10.1073/pnas.73.7.2424
  27. Rong P, Bennie AM, Epa WR, Barrett GL. Nerve growth factor determines survival and death of PC12 cells by regulation of the bcl-x, bax, and caspase-3 genes. J Neurochem. 1999;72: 2294-300. https://doi.org/10.1046/j.1471-4159.1999.0722294.x
  28. Quirk SM, Harman RM, Cowan RG. Regulation of fas antigen (Fas, CD95)-mediated apoptosis of bovine granulosa cells by serum and growth factors. Biol Reprod. 2000;63:1278-1284. https://doi.org/10.1095/biolreprod63.5.1278
  29. Huh J. Tonguibogam. seoul:Hyesungsa. 1994.
  30. Lee SD, Kim KS. The effect of Achyranthis Radix and Apitoxin Aqua- acupuncture on cellular immune responses to LPS-induced arthritis in mice. J Kor Acupuncture & Moxibustion Society. 1999;16:287-315.
  31. Do WS, Kim KH, Kim KS. Effect of aqua-acupuncture(Cortex Ulmi Pumilae, Ramulus Cinnamomum, Radix achyranthis, Apitoxin.Calculus Bovis.Fel Ursi.Moschus compound) in mice with lipopolysaccharide induced arthritis. J Kor Acupuncture & Moxibustion Society. 2001;18:157-69.
  32. Li XP. Experimental study on anti-senility of the 4 famous Chinese herbs produced in Huaiqing area. Zhong Xi Yi Jie He Za Zhi. 1991;11: 486-7.
  33. Jung SM, Choi SI, Park SM, Heo TR. Antimicrobial effect of Achyranthes japonica Nakai extracts against Clostrium difficile. Kor J Food Sci Technol. 2007;39:564-8.
  34. Eguchi Y, Shimizu S, Tsujimoto Y. Intracellular ATP levels determine cell death fate by apoptosis or necrosis. Cancer Res. 1997;57:1835-40.
  35. Gottlieb RA, Gruol DL, Zhu JY, Engler RL. Preconditioning rabbit cardiomyocytes. Role of pH, vacuolar proton ATPase, and apoptosis. J Clin Invest. 1996;97:2391-98. https://doi.org/10.1172/JCI118683
  36. Danno K, Horio T. Formation of UV-induced apoptosis relates to the cell cycle. Br J Dermatol. 1992;107:423-428. https://doi.org/10.1111/j.1365-2133.1982.tb00385.x
  37. Ding Y, Li J, Luan X, Ding YH, Lai Q, Rafols JA, Phillis JW, Clark JC, Diaz FG. Exercise pre-conditioning reduces brain damage in ischemic rats that may be associated with regional angiogenesis and cellular overexpression of neurotrophin. Neuroscience. 2004;124:583-591. https://doi.org/10.1016/j.neuroscience.2003.12.029
  38. Song HY, Park YK. Neuroprotective effect of modified Boyanghwano-Tang and the major medicinal plants, Astragali Radix and Salviae Miltiorrhizae Radix on ischemic stroke in rats. Kor J Herbology. 2010;25(2):71-79.

피인용 문헌

  1. Neuroprotective effects of some herbal medicine plant extract against ischemia·reperfusion-induced cell death in SK-N-SH neuronal cells vol.28, pp.2, 2013, https://doi.org/10.6116/kjh.2013.28.2.45