Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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In vitro shoot regeneration from leaf tissue of "Whangkeumbae" pear(Pyrus pyrifolia Nakai)

황금배(Pyrus pyrifolia Nakai) 잎 조직으로부터 기내 신초 재분화

  • Chun, Jae An (National Institute of Horticultural & Herbal Science, RDA) ;
  • Do, Kyung Ran (National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Se Hee (National Institute of Horticultural & Herbal Science, RDA) ;
  • Cho, Kang-Hee (National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Hyun Ran (National Institute of Horticultural & Herbal Science, RDA) ;
  • Hwang, Hae Sung (National Institute of Horticultural & Herbal Science, RDA) ;
  • Shin, Il Sheob (National Institute of Horticultural & Herbal Science, RDA)
  • 천재안 (농촌진흥청 국립원예특작과학원) ;
  • 도경란 (농촌진흥청 국립원예특작과학원) ;
  • 김세희 (농촌진흥청 국립원예특작과학원) ;
  • 조강희 (농촌진흥청 국립원예특작과학원) ;
  • 김현란 (농촌진흥청 국립원예특작과학원) ;
  • 황해성 (농촌진흥청 국립원예특작과학원) ;
  • 신일섭 (농촌진흥청 국립원예특작과학원)
  • Received : 2012.11.09
  • Accepted : 2012.11.27
  • Published : 2012.12.31
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Abstract

In order to establish an efficient adventitious shoot regeneration conditions from leaf explants for Asian pear 'Whangkeumbae', the effect of concentration and kinds of plant growth regulator and carbon source was investigated. Leaf explants of cultures grown on Murashige and Skoog (MS) medium containing 8 g/L plant agar were used. When the medium contained 0.25 mg/L thidiazuron (TDZ) and 0.3 mg/L indolebutyric acid (IBA), the adventitious shoot regeneration rate (ASRR) was greater as 61.1% than others treated and higher TDZ concentrations (2.5 and 5 mg/L) treatment significantly reduced the ASRR. As the effect of IBA and indoleacetic acid (IAA) concentration on the ASRR, 0.5 mg/L TDZ plus different concentration of IAA exhibited relatively high ASRR and 0.5 mg/L TDZ plus 0.3 mg/L IAA showed the highest ASRR of 76.7%. Also the effect of sucrose and sorbitol as carbon source on regeneration was examined. The highest ASRR and the most shoots per explants averaged 94.4% and 3.49 by treatment of 30 mg/L sorbitol, respectably. Sorbitol is considered better carbon source than sucrose for shoot regeneration of 'Whangkeumbae' pear.

본 연구는 '황금배'('Whangkeumbae')의 엽 절편체로부터 형질전환 체계 확립을 위한 효율적인 재분화 체계 확립을 위해 식물 생장조절제와 주요 탄소원인 sucrose, sorbitol이 식물체 재분화에 미치는 영향을 조사하기 위해 수행되었다. 잎 절편체를 MS 배지에 TDZ 농도를 각각 0.1, 0.25, 0.5, 1, 2.5, 5 mg/L으로 하여 IBA 0.3 mg/L을 혼용 처리한 결과 TDZ 0.25 mg/L 농도에서 61.1%의 가장 양호한 재분화율을 보였으며 TDZ 2.5 mg/L 이상의 농도에서는 신초의 재분화율이 급격히 감소하였다. IBA와 IAA 농도에 의한 신초의 재분화율은 TDZ 0.5 mg/L와 IAA 혼용 처리한 구에서 전체적으로 높은 신초의 재분화율을 보였으며 특히 TDZ 0.5 mg/L + IAA 0.3 mg/L 처리구에서 76.7%의 가장 높은 재분화율을 보였다. 서로 다른 탄소원으로써 sucrose와 sorbitol의 영향에 대하여 15 g/L, 30 g/L 농도로 처리한 결과 sorbitol 30 mg/L에서 가장 높은 재분화율을 보였고 또한 절편체 당 신초의 수도 3.5개로 가장 양호하였다. 따라서 sorbitol이 sucrose보다 '황금배'의 신초의 재분화에 있어서 효과적이었다.

Keywords

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