Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Comparison of transcriptome analysis between red flash peach cultivar and white flash peach cultivar using next generation sequencing

Next generation sequencing 방법을 이용한 적육계 복숭아와 백육계 복숭아의 전사체 분석

  • Kim, Se Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Nam, Eun Young (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Cho, Kang-Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Shin, Il Sheob (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Hyun Ran (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Hwang, Hae Seong (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
  • 김세희 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 남은영 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 조강희 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 신일섭 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 김현란 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 황해성 (농촌진흥청 국립원예특작과학원 과수과)
  • Received : 2012.11.09
  • Accepted : 2012.11.23
  • Published : 2012.12.31
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Abstract

Differences of gene expression between red flash peach cultivar and white flash peach cultivar were investigated by Nest-generation sequencing (NGS). EST from the red flash peach cultivar and white flash peach cultivar were selected for nucleotide sequence determination and homology searches. The levels of transcripts coding for proteins involved in pathogenesis related proteins, temperature stress, ethylene signal pathway were significantly higher in white flash peach cultivar than in red flash peach cultivar. On the other hand, the up-regulation of proteins involved in anthocyanin and flavonol biosynthesis and protein degradation and sorbitol metabolism were observed in red flash peach cultivar. Chalcone synthase was preferentially expressed in the red flesh peach cultivar, agreeing with the accumulation of anthocyanin and expression of other previously identified genes for anthocyanin biosynthesis. Anthocyanin pathway related genes CHS, F3H, DFR, LDOX, UFGT differentially expressed between red flash peach cultivar and white flash peach cultivar. These results suggest that red flash peach cultivar and white flash peach cultivar have different anthocyanin biosynthesis regulatory mechanisms.

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References

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