Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Sequence Coverage Enhancement Using Magnetic Nanoparticles in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometric Protein Analysis

  • Park, Eun-Hye (Department of Chemistry, Seoul National University) ;
  • Song, Jin-Su (Department of Chemistry, Seoul National University) ;
  • Kim, Hie-Joon (Department of Chemistry, Seoul National University)
  • Received : 2011.11.30
  • Accepted : 2012.01.18
  • Published : 2012.03.20
Download PDF
⟨ Previous Next ⟩

Abstract

Magnetic nanoparticles (MNPs) treated with phosphoric acid were used to improve sequence coverage in protein identification by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Sample solution of tryptic peptides from proteins was mixed with the MNPs, and the MNPs were separated from the supernatant using a magnet. MALDI mass spectra obtained separately from the supernatant and the MNPs were distinctly different and complementary to each other. Combination of the two spectra led to a significantly increased sequence coverage.

Keywords

References

  1. Meyer, B.; Papasotiriou, D. G.; Karas, M. Amino Acids 2011, 41, 291. https://doi.org/10.1007/s00726-010-0680-6
  2. Sanaki, T.; Suzuki, M.; Lee, S. H.; Goto, T.; Oe, T. Anal. Methods- Uk 2010, 2, 1144. https://doi.org/10.1039/c0ay00215a
  3. Zhang, X. M.; Tang, J.; Liu, Y. C.; Qi, D. W.; Yao, G. P.; Deng, C. H. Proteomics 2009, 9, 5046. https://doi.org/10.1002/pmic.200900033
  4. Kim, J.; Kim, B. C.; Lopez-Ferrer, D.; Lee, S. M.; Ahn, H. K.; Nair, S.; Kim, S. H.; Kim, B. S.; Petritis, K.; Camp, D. G.; Grate, J. W.; Smith, R. D.; Koo, Y. M.; Gu, M. B. Proteomics 2009, 9, 1893. https://doi.org/10.1002/pmic.200800591
  5. Lee, J. H.; Kim, Y. S.; Ha, M. Y.; Lee, E. K.; Choo, J. B. J. Am. Soc. Mass Spectr. 2005, 16, 1456. https://doi.org/10.1016/j.jasms.2005.04.005
  6. Yang, P. Y.; Wei, L. M.; Zhang, W.; Lu, H. J. Talanta 2010, 80, 1298. https://doi.org/10.1016/j.talanta.2009.09.029
  7. Kong, X. L.; Huang, L. C. L.; Hsu, C. M.; Chen, W. H.; Han, C. C.; Chang, H. C. Anal. Chem. 2005, 77, 259. https://doi.org/10.1021/ac048971a
  8. Jeng, J. Y.; Lin, M. F.; Cheng, F. Y.; Yeh, C. S.; Shiea, J. T. Rapid Commun. Mass Sp. 2007, 21, 3060. https://doi.org/10.1002/rcm.3191
  9. Kumarathasan, P.; Mohottalage, S.; Vincent, R. J. Aoac. Int. 2009, 92, 1652.
  10. Shaw, S. Y.; Chen, Y. J.; Ou, J. J.; Ho, L. Enzyme Microb. Tech. 2006, 39, 1089. https://doi.org/10.1016/j.enzmictec.2006.02.025
  11. Lee, A.; Yang, H. J.; Lim, E. S.; Kim, J.; Kim, Y. Rapid Commun. Mass Sp. 2008, 22, 2561. https://doi.org/10.1002/rcm.3652
  12. Chiu, Y. C.; Chen, Y. C. Anal. Lett 2008, 41, 260. https://doi.org/10.1080/00032710701792653
  13. Shokrollahi, H.; Taghvaei, A. H.; Janghorban, K. J. Alloy Compd. 2009, 481, 681. https://doi.org/10.1016/j.jallcom.2009.03.074

Cited by

  1. Protein Analysis Using a Combination of an Online Monolithic Trypsin Immobilized Enzyme Reactor and Collisionally-Activated Dissociation/Electron Transfer Dissociation Dual Tandem Mass Spectrometry vol.33, pp.10, 2012, https://doi.org/10.5012/bkcs.2012.33.10.3233