Journal of Korean Medicine for Obesity Research (한방비만학회지)

The Society of Korean Medicine for obesity Research (한방비만학회)
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Study on Biological Effect of Euiiin-tang(y$\grave{i}$y$\check{i}$r$\acute{e}$n-t$\bar{a}$ng) Extracts by Extraction Methods

의이인탕 추출방법에 따른 생리활성 연구

  • Park, Kyung-Moo (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Ga-Chon University) ;
  • Yeom, Do-Sung (Dept. of Oriental Rehabilitation Medicine, Nazareth Hospital of Oriental Medicine) ;
  • Kim, Hak-Ju (SeoJin Biotech Co., Ltd.) ;
  • Ko, Seong-Gyu (Dept. of Preventive Medicine, College of Korean Medicine, Kyung-Hee University) ;
  • Lim, Hyung-Ho (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Ga-Chon University) ;
  • Song, Yun-Kyung (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Ga-Chon University)
  • 박경무 (가천대학교 한의과대학 한방재활의학교실) ;
  • 염도성 (나사렛 한방병원 한방재활과) ;
  • 김학주 ((주) 서진바이오텍) ;
  • 고성규 (경희대학교 한의과대학 예방의학교실) ;
  • 임형호 (가천대학교 한의과대학 한방재활의학교실) ;
  • 송윤경 (가천대학교 한의과대학 한방재활의학교실)
  • Received : 2012.12.13
  • Accepted : 2012.12.23
  • Published : 2012.12.30
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Abstract

Objectives: The purpose of this study is to investigate biological effects of Euiiin-tang(Y$\grave{i}$y$\check{i}$r$\acute{e}$n-t$\bar{a}$ng) extracts by extraction methods. Methods: To investigate the effects of anti-oxidative and anti-inflammatory activity, we measured DPPH radical scavenging assay, collagenase inhibition assay and COX-2 inhibition assay. Results: In case of measuring anti-oxidative and anti-inflammatory activities of Euiiin-tang(Y$\grave{i}$y$\check{i}$r$\acute{e}$n-t$\bar{a}$ng) by collagenase and COX-2 inhibition activity, it came out that Euiiin-tang(Y$\grave{i}$y$\check{i}$r$\acute{e}$n-t$\bar{a}$ng) was unable to inhibit collagenase, but was effective to inhibit COX-2. Cytotoxicity analysis of Euiiin-tang(Y$\grave{i}$y$\check{i}$r$\acute{e}$n-t$\bar{a}$ng) extracts by MTT assay principles, there was no cytotoxicity in aqueous and 50% ethanol extracts in any level of concentration. Conclusions: This results suggest that Euiiin-tang(Y$\grave{i}$y$\check{i}$r$\acute{e}$n-t$\bar{a}$ng) extracts have anti-oxidative and anti-inflammatory activities.

Keywords

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