Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
권호 표지
DOI QR코드

DOI QR Code

Determination and Ethylene Oxide(EO) Separation of Polysorbates by RP-HPLC

역상 HPLC에 의한 polysorbates의 산화에틸렌(EO) 분리 및 정량분석

  • 이용화 (호서대학교 융합기술연구소)
  • Received : 2012.11.19
  • Accepted : 2012.12.16
  • Published : 2012.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

The EO separation and quantitative determination of polysorbate 20, polysorbate 40, polysorbate 60 and polysorbate 80 was carried out by reversed phase HPLC. The water/acetonitrile was used for the mobile phase of gradient conditions. An YMC Pack Ph ($250mm{\times}4.6mm$ i.d., $5{\mu}m$) and Phenomenex C4 ($250mm{\times}4.6mm$ i.d., $5{\mu}m$) and the selected ELSD detector was applied. The analysis results of HPLC showed good linearity with correlation coefficient of $r^2$=0.997 in the rage of $180.2{\sim}980.5{\mu}g/mL$ and detection limit.

역상 HPLC에 의한 polysorbate 20, polysorbate 40, polysorbate 60, polysorbate 80의 ethylene oxide(EO)분리 및 간편하고 빠른 정량분석 방법을 개발하였다. 분석조건으로 분리관은 YMC Pack Ph ($250mm{\times}4.6mm$ i.d., $5{\mu}m$) 과 Phenomenex C4 ($250mm{\times}4.6mm$ i.d., $5{\mu}m$)을 사용하였고, 검출기는 ELSD를 사용하였으며, 이동상은 water/acetonitrile의 기울기 용리에 의해 분석되었다. 이때 검량선의 상관계수($r^2$)는 $180.2{\sim}980.5{\mu}g/mL$ 농도 범위에서 0.997이상 이었고, 검출한계, 정밀성이 우수하였다. 이 방법은 olysorbates의 산화에틸렌 분리분석 및 간편하고 빠르게 정량분석 가능함을 보여 주었다.

Keywords

References

  1. W. G. Cutler and E. Kissa, Detergency : Theory and Technology, Surfactant Science Series, 20, 1, Marcel Dekker, New York (1987).
  2. A. M. Schwartz, The Physical Chemistry of Detergency ed. E. Matijevic, Surface Colloid Sci., 195, Wiley, New York (1972).
  3. C. A. Miller and P. Neogi, Interfacial Phenomena : Equilibrium and Dynamic Effects, Surfactant Science Series, 17, 150, Marcel Dekker, New York (1985).
  4. J. C. Lim, J. Kor. Ind. Eng. Chem., 6, 610 (1995).
  5. J. C. Lim, J. Kor. Ind. Eng. Chem., 8, 473 (1997).
  6. S. K. Lee, J. W. Han, B. H. Kim, P. G. Shin, S. K. Park, and J.C. Lim, J. Kor. Ind. Eng. Chem., 10, 537 (1999).
  7. H. K. Ko, B. D. Park, and J. C. Lim, J. Kor. Ind. Eng. Chem., 11, 679 (2000).
  8. J. G. Lee, S. S. Bae, I. S. Cho, S. J. Park, B. D. Park, S. K. Park, and J. C. Lim, J. Kor. Ind. Eng. Chem., 16, 664 (2005).
  9. J. G. Lee, S. S. Bae, I. S. Cho, S. J. Park, B. D. Park, S. K. Park, and J. C. Lim, J. Kor. Ind. Eng. Chem., 16, 677 (2005).
  10. J. C. Lim, J. Kor. Ind. Eng. Chem., 16, 778 (2005).
  11. S. Lee, B. J. Kim, J. G. Lee, and J. C. Lim, J. Kor. Ind. Eng. Chem., 16, 778 (2011).
  12. D. H. Daniels, C. R. Warner, S. Selim, J. Assoc. Off. Anal. Chem. 65, 162, (1982).
  13. J. M. Murphy, C. C. Scott, Analyst 94, 481(1969).
  14. T. H. Tani, J. M. Moore, T. W. Patapoff, J. Chromatogr. A 786, 99 (1997).
  15. C. Crescenzi, A. Di Crocia, R. Samperi, Anal. Chem. 67, 1797 (1995).
  16. M. Castillo, F. Ventura and D. Barcel, Waste Manage. 19, 101 (1999).
  17. K. A. Krogh, K. V. Vejrup, B. B. Mongensen and B. Halling-Sørensen, J. Chromatogr. A 957, 45 (2002).
  18. M. M. Ahel and W. Giger., Anal. Chem. 2584 (1985).
  19. M. Takino, S. Daishima, K. Yamaguchi, J. Chromatogr. A 904, 65(2000).
  20. A. M. Rothman., J. Chromatogr. 253, 283 (1982).
  21. C. Sun, M. Baird, Hamish A. Anderson, Donald L. Brydon, J. Chromatogr. A, 145 (1997).
  22. A Marcomini, M. Zanette, J. Chromatogr. A 193 (1996).
  23. M. Ahel and W. Giger. Anal. Chem. 57, 2584 (1985).
  24. A. M. Rothman. J. Chromatogr. 283 (1982).
  25. Cheng Sun, Margot Baird, Hamish A. Anderson, Donald L. Brydon, J. Chromatogr. A , 145 (1997).
  26. R. E. A. Escott and M. Mortimer, J. Chromatogr, 423 (1991).
  27. Chin-Yuan Cheng, Wang-Hsien Ding, J. Chromatogr, A, 143 (2002).
  28. F. I. Portet, C. Treiner, P. L. Desbine. J. Chromatogr. A878, 99 (2000).
  29. Marieta Balcan, ColloidsSurf. A, 221, 1 (2003).
  30. Klaus Rissler. J. Chromatogr. A, 1 (1996).
  31. P. L. Desbene, F. I. Portet, G. J. Goussot. J. Chromatogr. A, 730, 209 (1996).
  32. G. Cretier, C. Podevin and J. -L. Rocca. J. Chromatogr. A, 305 (2000).
  33. Lakshmy M. Nair, Norma V. Stephens, Sarah Vincent, Neervalur Raghavan, Patrick J. Sand. D, J. Chromatogr. A, 81 (2003).
  34. Hong Soon Park, Ho Ryul Ryu, Choong Kyun Rhee. Talanta, 70, 481 (2006).
  35. Hong Soon Park, Choong Kyun Rhee. J. Chromatogr. A, 289 (2004).