Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Biological Activities of the Essential Oil from Angelica acutiloba

  • Roh, Junghyun (College of Pharmacy, Duksung Women's University) ;
  • Lim, Hyerim (College of Pharmacy, Duksung Women's University) ;
  • Shin, Seungwon (College of Pharmacy, Duksung Women's University)
  • Received : 2012.10.31
  • Accepted : 2012.11.20
  • Published : 2012.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Angelica acutiloba is one of the most intensively cultivated medicinal plants in Korea. The roots of this plant have been used as an important herbal drug, especially for the treatment of various female disorders, as the traditional therapy in Korea and other Asian countries. Consumption of its fresh leaves as a healthy vegetable has recently increased. In this study, essential oil fractions were extracted from the roots and leaves of this plant by steam distillation. Compositions of the two oils were compared by gas chromatography-mass spectrometry (GC-MS). The antibacterial activities of the essential oil were determined against three strains of Escherichia coli. DPPH radical scavenging and reducing power tests were performed to evaluateits antioxidant activities. The cytotoxic activities of the essential oil against a human breast and a uterine cancer cell line were estimated by MTT tests. Additionally, the morphological changes after treatment of the oil fraction were observed under a microscope. The essential oil fraction and its main components, Z-ligustilide and butylidene phthalide, inhibited the growth of three E. coli strains examined, with minimum inhibiting concentrations (MICs) ranging from 1.0 mg/ml to 8.0 mg/ml. Additionally, the essential oil fraction of A. acutiloba exhibited significant DPPH free radical scavenging activity and reducing power. Significant cytotoxic activities of the A. acutiloba essential oil were observed for human uterine (Hela) and breast (MCF-7) cancer cell lines.

Keywords

References

  1. Base, K.H.C. and Buchbauer, G., Handbook of Essential Oils, CRC Press, New York, 2010.
  2. Burt, S., Essential oils: their antibacterial properties and potential applications in foods-a review. Int. J. Food Microbiol. 94, 223-253 (2004). https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
  3. Blois, M.S., Antioxidant determination by use of a stable free radical. Nature 181, 1199-1200 (1958). https://doi.org/10.1038/1811199a0
  4. Chang, H.M. and But, P.P.H., Pharmacology and Applications of Chinese Materia Medica, World Scientific, Singapore, 1986.
  5. Choi, S.K. and Yang, D.C., A basic study on utilization of Angelica acutiloba Kitag (Tanggui). Korean J. Plant Res. 8, 230-234 (2005).
  6. Du, L., Wang, X., Cai, C, and Wang, T., Constituent analysis of essential oils from radix of Angelica acutiloba. Zong Yao Cai 25, 477-478 (2002).
  7. Elmastas, M., Isildak, O., Turkekul, I., and Temur, N., Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms. Journal of Food Compos. Anal. 20, 337-345 (2007). https://doi.org/10.1016/j.jfca.2006.07.003
  8. Kang, S.A., Jang, K.H., Lee, J.E., Ahn, D.K., and Park S.K., Differences of hematopoietic effects of Angelica gigas, A. sinensis and A. acutiloba extract on cyclophosphamide-induced anemic rats. Korean J. Food Sci. Technol. 35, 1204-1208 (2003).
  9. Kim, A.R., Lee, J.J., and Lee, M.Y., Antioxidative effect of Angelica acutiloba Kitagawa ethanol extract. J. Life Sci. 19, 117-122 (2009). https://doi.org/10.5352/JLS.2009.19.1.117
  10. Kiran, S., Ratho, R.K., Sharma, P., Harjai, K., Capalash, N., and Tiwari, R.P., Effect of black tea (Camellia sinensis) on virulence traits of clinical isolates of Shigella dysenteriae and Escherichia coli EPEC P2 1265 strain. Eur. Food Res. Technol. 231, 763-770 (2010). https://doi.org/10.1007/s00217-010-1328-1
  11. Lim, H.R. and Shin, S.W., Activities of essential oils from Perilla frutescens var. acuta against antibiotic-susceptible and-resistant Vibrio and Salmonella species. Nat. Prod. Sci. 17, 296-302 (2011).
  12. Liu, C., Tseng, A., and Yang, S., Chinese Herbal Medicine, CRC Press, Boca Raton, 2005.
  13. Liu, D.M., Sheng, J.W., Qi, H.M., Zhang, W.F., Han, C.M., and Xin, X.L., Antioxidant activity of polysaccharides extracted from Athyrium multidentatum (Doll) Ching. J. Med. Plants Res. 5, 3061-3066 (2011).
  14. Owens, R.C. and Ambrose, P.G., Antimicrobial safety: focus on fluoroquinolones. Clin. Infect. Dis. 41, 144-157 (2005). https://doi.org/10.1086/428055
  15. Park, C.H., Juliani, H.R., Park, H.W., Yu, H.S., and Simon, J.E., Comparison of essential oil composition between Angelica gigas and Angelica acutiloba. Korean J. Plant. Res. 6, 183-187 (2003).
  16. Race, P.R., Solovyova, A.S., and Banfield, M.J., Conformation of the EPEC Tir protein in solution: Investigating the impact of serine phosphorylation at positions 434/463. Biophys. J. 93, 586-596 (2007). https://doi.org/10.1529/biophysj.106.101766
  17. Scholar, E.M. and Pratt, W.B., The Antimicrobial Drugs, Oxford, New York, 2000.
  18. Shin, S. and Lim, S., Antifungal effects of herbal essential oils alone and in combination with ketoconazole against Trichophyton spp. J. Appl. Microbiol. 97, 1289-1296 (2004). https://doi.org/10.1111/j.1365-2672.2004.02417.x
  19. Sim, Y. and Shin, S., Combinatorial anti-Trichophyton effects of Ligusticum chuanxiong essential oil components with antibiotics. Arch. Pharm. Res. 31, 497-502 (2008). https://doi.org/10.1007/s12272-001-1184-7