Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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TGF-β1 Expression by Proliferated Keratinocytes in the Skin of E-Irradiated Mice

E-ray를 조사한 쥐의 피부에서 증식된 keratinocyte에 의한 TGF-β1 발현

  • Yoon, A-Ran (Department of Medical Lifescience, College of Medicine, The Catholic University of Korea) ;
  • Kim, Do-Nyun (Department of Medical Lifescience, College of Medicine, The Catholic University of Korea) ;
  • Seo, Min-Koo (Department of Medical Lifescience, College of Medicine, The Catholic University of Korea) ;
  • Oh, Sang-Taek (Department of Medical Lifescience, College of Medicine, The Catholic University of Korea) ;
  • Seo, Jung-Seon (Department of Medical Lifescience, College of Medicine, The Catholic University of Korea) ;
  • Jun, Se-Mo (Department of Medical Lifescience, College of Medicine, The Catholic University of Korea) ;
  • Cha, Jung-Ho (Department of Anatomy, College of Medicine, The Catholic University of Korea) ;
  • Lee, Seung-Deok (Department of Oriental Medicine, The Graduate School of Dongguk University) ;
  • Lee, Suk-Kyeong (Department of Medical Lifescience, College of Medicine, The Catholic University of Korea)
  • 윤아란 (가톨릭대학교 의과대학 의생명과학교실 면역생물학연구소) ;
  • 김도년 (가톨릭대학교 의과대학 의생명과학교실 면역생물학연구소) ;
  • 서민구 (가톨릭대학교 의과대학 의생명과학교실 면역생물학연구소) ;
  • 오상택 (가톨릭대학교 의과대학 의생명과학교실 면역생물학연구소) ;
  • 서정선 (가톨릭대학교 의과대학 의생명과학교실 면역생물학연구소) ;
  • 전세모 (가톨릭대학교 의과대학 의생명과학교실 면역생물학연구소) ;
  • 차정호 (가톨릭대학교 의과대학 해부학교실) ;
  • 이승덕 (동국대학교 한의예과) ;
  • 이숙경 (가톨릭대학교 의과대학 의생명과학교실 면역생물학연구소)
  • Received : 2011.11.16
  • Accepted : 2011.11.27
  • Published : 2012.02.28
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Abstract

In this study, we established a radiodermatitis animal model and investigated the change in immune cell proportions in the secondary lymphoid organs. The cells responsible for the increased transforming growth factor-${\beta}1$ (TGF-${\beta}1$) and interleukin-10 (IL-10) production in the lesions following irradiation were also investigated. The radiodermatitis model was constructed by locally exposing the posterior dorsal region of hairless-1 (HR-1) mice to 10 Gy electron (E)-ray/day for six consecutive days. The change in immune cell proportions was analyzed by FACS. Immunohistochemistry was carried out to detect the expression of cytokines and cell-specific markers in the skin. The proportions of antigen-presenting cells, T cells, and B cells in the lymph nodes and spleen were affected by E-irradiation. After irradiation, TGF-${\beta}1$ and IL-17 were co-localized in the papillary region of the dermis with keratin-14 (K-14)-positive cells rather than with regulatory T cells (Treg). IL-10 was not co-stained with Treg, T helper 17 (Th17) cells, dendritic cells, or macrophages. Our data indicate that TGF-${\beta}1$ is over-expressed mainly by proliferated keratinocytes in the lesions of a radiodermatitis animal model.

우리는 방사선피부염 동물 모델을 확립하여, 이차 면역 기관에서의 면역 세포 비율 변화를 관찰하였다. 또한, 방사선 조사에 의한 병소에서 transforming growth factor-${\beta}1$ (TGF-${\beta}1$)과 interlukin-10 (IL-10)의 발현을 증가시킨 세포를 분석하였다. Hairless-1 (HR-1) 쥐의 posterior dorsal 부위에 6 일간 매일 10 Gy 씩 electron (E)-ray를 국부 조사하여 방사선피부염 모델을 만들었다. FACS를 이용하여 면역 세포 비율의 변화를 분석한 결과 비장과 림프절에 존재하는 항원제시세포와 T 세포 및 B 세포들의 비율이 E-irradiation에 의해 영향을 받았다. 피부에서 세포 특이적인 마커와 사이토카인들의 발현 양상은 면역형광염색법으로 확인하였다. 방사선 조사 후, TGF-${\beta}1$과 interlukin-17 (IL-17)은 regulatory T 세포(Treg)보다 keratin-14 (K-14)를 발현하는 진피의 끝부분에서 높게 발현되었다. Interlukin-10 (IL-10)는 Treg 뿐만 아니라 T helper 17 (Th17) 세포, dendritic 세포, macrophage 중 어느 것과도 같은 위치에서 검출되지 않았다. 우리의 데이터는 방사선피부염 동물 모델의 병소 안에서, TGF-${\beta}1$이 증식된 keratinocyte에 과발현된다는 것을 나타낸다.

Keywords

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