Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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The Aqueous Extract of Rubus coreanus Miquel Improves Scopolamine-Induced Memory Impairment in ICR Mice

마우스에서 복분자 물추출물의 기억력 감퇴에 대한 개선 효과

  • Choi, Mi-Ran (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University) ;
  • Lee, Min-Young (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University) ;
  • Hong, Ji-Eun (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University) ;
  • Lee, Jae-Yong (Dept. of Biochemistry, College of Medicine, Hallym University) ;
  • Chun, Jang-Woo (Research and Development Center, Hitejinro Co., Ltd.) ;
  • Kim, Tae-Hwan (Research and Development Center, Hitejinro Co., Ltd.) ;
  • Shin, Hyun-Kyung (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University) ;
  • Kim, Eun-Ji (Center for Efficacy Assessment and Development of Functional Foods and Drugs, Hallym University)
  • 최미란 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 이민영 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 홍지은 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 이재용 (한림대학교 의과대학 생화학교실) ;
  • 전장우 (하이트진로주식회사 중앙연구소) ;
  • 김태환 (하이트진로주식회사 중앙연구소) ;
  • 신현경 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터) ;
  • 김은지 (한림대학교 식의약품의 효능평가 및 기능성소재개발센터)
  • Received : 2011.11.15
  • Accepted : 2011.12.27
  • Published : 2012.02.29
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Abstract

In the present study, we examined the effect of the aqueous extract of Rubus coreanus Miquel (RCM-Ex) on scopolamine-induced memory impairment in male ICR mice. Mice were fed the diet containing 100 mg/kg body weight/day of RCM-Ex for 4 weeks. To induce amnesia, scopolamine (an antagonist of muscarinic acetylcholine receptor, 1 mg/kg of body weight) was intraperitoneally injected into mice 30 min before starting the behavior tests. RCM-Ex reversed scopolamine-induced memory impairments in mice as evidence by the passive avoidance test and Morris water maze test. In addition, acetylcholineasterase activities were decreased in the brains of mice treated with RCM-Ex. These results suggest that RCM-Ex may be an effective agent for the prevention of the memory impairment induced by cholinergic dysfunction.

현재까지 복분자가 인지능력 및 기억력에 미치는 영향에 대해 연구된 바가 없다. 본 연구에서는 복분자 물추출물이 기억력에 미치는 영향을 조사하기 위해 복분자 물추출물을 100 mg/kg body weight/day로 4주간 투여한 후 스코폴라민(1 mg/kg body weight)을 복강 주사하여 기억력 감퇴를 유발한 후 수동회피시험과 수중미로시험을 실시하여 복분자물추출물의 기억력 개선 효과를 평가하였다. 수동회피실험에서 스코폴라민으로 기억력 감퇴를 유도한 경우 밝은 방에서의 체류시간이 현저히 짧아졌고 복분자 물추출물을 섭취한 군에서는 밝은 방에서의 체류 시간이 스코폴라민 투여군에 비해 현저히 증가하였다. 또한 수중미로시험에서도 스코폴라민 투여군의 도피대를 찾아가는 탈출잠복기가 정상대조군에 비해 현저히 증가하였고 복분자 물추출물 투여군에서는 스코폴라민 군에 비해 탈출잠복기가 유의적으로 감소하였다. 대뇌피질에서 기억력에서 중요한 역할을 하는 acetylcholinesterase활성은 스코폴라민에 의해 증가하고, 복분자 물추출물에 의해 감소하였다. 이상의 결과는 복분자 물추출물이 항콜린제인 스코폴라민에 의해 유도된 기억력 감퇴를 효과적으로 개선함을 보여주며, 이는 acetylcholinesterase의 활성을 저해함으로써 이루어짐을 나타낸다. 향후 더 많은 연구가 필요하나 본 연구는 복분자 물추출물을 기억력 개선 또는 증강제로 개발할 수 있는 가능성을 제시한다.

Keywords

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