Bulletin of the Korean Chemical Society

  • 제33권1호
  • /
  • Pages.177-182
  • /
  • 2012
  • /
  • 0253-2964(pISSN)
  • /
  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
권호 표지
DOI QR코드

DOI QR Code

Simultaneous Determination of Baicalein, Baicalin, Wogonin, and Wogonoside in Rat Plasma by LC-MS/MS for Studying the Pharmacokinetics of the Standardized Extract of Scutellariae Radix

  • Chung, Hye-Jin (Neuro-Medicine Center, Korea Institute of Science and Technology) ;
  • Lim, Sun-Young (College of Pharmacy, Hanyang University) ;
  • Kim, In-Sook (College of Pharmacy, Hanyang University) ;
  • Bu, Young-Min (Department of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Ho-Cheol (Department of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Dong-Hyun (Department of Pharmacology and PharmacoGenomics Research Center, College of Medicine, Inje University) ;
  • Yoo, Hye-Hyun (College of Pharmacy, Hanyang University)
  • 투고 : 2011.10.21
  • 심사 : 2011.11.17
  • 발행 : 2012.01.20
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

A new composition of standardized Scutellariae Radix extract (HPO12) was developed for treatment of Alzheimer's disease. For the preclinical pharmacokinetic study of HPO12, a rapid, sensitive, and selective LCMS/MS method was developed and validated for the simultaneous determination of 4 bioactive compounds, baicalein, baicalin, wogonin, and wogonoside. After extraction with ethylacetate, chromatographic analysis was performed on a Thermo $C_{18}$ column ($150mm{\times}2.1mm$, $3{\mu}m$) with a mobile phase consisting of 0.1% formic acid (A) and 0.1% formic acid in 95% acetonitrile (B) by using gradient elution at a flow rate of $250{\mu}L/min$. Analytes introduced to a mass spectrometer were monitored by multiple reaction monitoring (MRM) in positive ion mode. Using $25{\mu}L$ of plasma sample, the method was validated over the following concentration ranges: 25-5000 ng/mL for baicalein, 20-40000 ng/mL for baicalin, 1-1000 ng/mL for wogonin, and 5-10000 ng/mL for wogonoside. The intra- and inter-day precision and accuracy of the quality control samples at the 4 concentrations showed $\leq$ 13.7% relative standard deviation (RSD) and 86.6-105.5% accuracy. The method was successfully applied to determine the concentrations of baicalein, baicalin, wogonin, and wogonoside in rat plasma after intraperitoneal and oral administrations of HPO12.

키워드

참고문헌

  1. Lin, C. C.; Shieh, D. E. Am. J. Chin. Med. 1996, 24, 31. https://doi.org/10.1142/S0192415X96000050
  2. Wang, Y. S.; Deng, W. L.; Que, C. S. Pharmacology and Application of Traditional Chinese Medicine; Human Health Press: Beijing, China, 1998; pp 972-982, pp 1004-1029.
  3. Kondo, Y.; Kondo, F.; Asanuma, M.; Tanaka, K.; Ogawa, N. Neurochem. Res. 2000, 25, 205. https://doi.org/10.1023/A:1007515318434
  4. Xu, J.; Murakami, Y.; Matsumoto, K.; Tohda, M.; Watanabe, H.; Zhang, S.; Yu, Q.; Shen, J. J. Ethnopharmacol. 2000, 73, 405. https://doi.org/10.1016/S0378-8741(00)00303-2
  5. Gao, Z.; Huang, K.; Yang, X.; Xu, H. Biochim. Biophys. Acta 1999, 1472, 643. https://doi.org/10.1016/S0304-4165(99)00152-X
  6. Lee, H.; Kim, Y. O.; Kim, H.; Kim, S. Y.; Noh, H. S.; Kang, S. S.; Cho, G. J.; Choi, W. S.; Suk, K. FASEB J. 2003, 17, 1943. https://doi.org/10.1096/fj.03-0057fje
  7. Tsai, T. H.; Liu, S. C.; Tsai, P. L.; Ho, L. K.; Shum, A. Y. C.; Chen, C. F. Br. J. Pharmacol. 2002, 137, 1314. https://doi.org/10.1038/sj.bjp.0704959
  8. Lai, M. Y.; Hsiu, S. L.; Chen, C. C.; Hou, Y. C.; Chao, P. D. Biol. Pharm. Bull. 2003, 26, 79. https://doi.org/10.1248/bpb.26.79
  9. Lai, M. Y.; Hsiu, S. L.; Tsai, S. Y.; Hou, Y. C.; Chao, P. D. J. Pharm. Pharmacol. 2003, 55, 205. https://doi.org/10.1211/002235702522
  10. Zuo, F.; Zhou, Z. M.; Zhang, Q.; Mao, D.; Xiong, Y. L.; Wang, Y. L.; Yan, M. Z.; Liu, M. L. Biol. Pharm. Bull. 2003, 26, 911. https://doi.org/10.1248/bpb.26.911
  11. Kim, Y. H.; Jeong, D. W.; Kim, Y. C.; Sohn, D. H.; Park, E. S.; Lee, H. S. Arch. Pharm. Res. 2007, 30, 260. https://doi.org/10.1007/BF02977703
  12. Feng, J.; Xu, W.; Tao, X.; Wei, H.; Cai, F.; Jiang, B.; Chen, W. J. Pharm. Biomed. Anal. 2010, 53, 591. https://doi.org/10.1016/j.jpba.2010.04.002
  13. Hou, Y. C.; Lin, S. P.; Tsai, S. Y.; Ko, M. H.; Chang, Y. C.; Chao, P. D. Planta Med. 2011, 77, 455. https://doi.org/10.1055/s-0030-1250433
  14. Li, C.; Zhang, L.; Lin, G.; Zuo, Z. J. Pharm. Biomed. Anal. 2011, 54, 750. https://doi.org/10.1016/j.jpba.2010.10.005
  15. Zan, B.; Shi, R.; Wang, T.; Wu, J.; Ma, Y.; Cheng, N. Biomed. Chromatogr. 2011, 25, 816. https://doi.org/10.1002/bmc.1521
  16. Akao, T.; Kawabata, K.; Yanagisawa, E.; Ishihara, K.; Mizuhara, Y.; Wakui, Y.; Sakashita, Y.; Kobashi, K. J. Pharm. Pharmacol. 2000, 52, 1563. https://doi.org/10.1211/0022357001777621
  17. http://www.fda.gov/downloads/Drugs/ GuidanceComplianceRegulatoryInformation/Guidances/ ucm070107.pdf.
  18. Saar, E.; Gerostamoulos, D.; Drummer, O. H.; Beyer, J. Anal. Bioanal. Chem. 2009, 393, 727. https://doi.org/10.1007/s00216-008-2498-6
  19. Xing, J.; Chen, X.; Zhong, D. Life Sci. 2005, 78, 140. https://doi.org/10.1016/j.lfs.2005.04.072

피인용 문헌

  1. Controlled Extraction of Flavonoids From Radix Scutellariae by Subcritical Water vol.44, pp.3, 2016, https://doi.org/10.1002/clen.201300193
  2. Development of an immunoassay using an anti-wogonin glucuronide monoclonal antibody vol.38, pp.5, 2017, https://doi.org/10.1080/15321819.2016.1273236
  3. Comparative intestinal bacteria-associated pharmacokinetics of 16 components of Shengjiang Xiexin decoction between normal rats and rats with irinotecan hydrochloride (CPT-11)-induced gastrointestinal toxicity in vitro using salting-out sample preparation and LC-MS/MS vol.7, pp.69, 2017, https://doi.org/10.1039/C7RA03521G
  4. An overview and comparison of a recombinant antigen-binding fragment and an antigen-binding fragment from a monoclonal antibody against wogonin glucuronide vol.71, pp.4, 2017, https://doi.org/10.1007/s11418-017-1100-y
  5. -glucuronide and wogonoside in rat plasma by UPLC-DAD and its application in pharmacokinetics of pure baicalin, Radix Scutellariae and Yinhuang granule vol.29, pp.12, 2015, https://doi.org/10.1002/bmc.3501
  6. Investigation on the Metabolism of Curcumin and Baicalein in Zebrafish by Liquid Chromatography-tandem Mass Spectrometry Analysis vol.15, pp.None, 2012, https://doi.org/10.2174/1573412915666190522083850
  7. Pharmacokinetics of B-Ring Unsubstituted Flavones vol.11, pp.8, 2019, https://doi.org/10.3390/pharmaceutics11080370
  8. Chemical profiling of Justicia vahlii Roth. (Acanthaceae) using UPLC-QTOF-MS and GC-MS analysis and evaluation of acute oral toxicity, antineuropathic and antioxidant activities vol.287, pp.None, 2012, https://doi.org/10.1016/j.jep.2021.114942