한국발생생물학회지:발생과생식 (Development and Reproduction)

  • 제16권4호
  • /
  • Pages.371-378
  • /
  • 2012
  • /
  • 2465-9525(pISSN)
  • /
  • 2465-9541(eISSN)
한국발생생물학회 (The Korean Society of Developmental Biology)
권호 표지

The Ascidian Numb Gene Involves in the Formation of Neural Tissues

  • Ahn, Hong Ryul (Dept. of Marine Molecular Biotechnology, Gangneung-Wonju National University) ;
  • Kim, Gil Jung (Dept. of Marine Molecular Biotechnology, Gangneung-Wonju National University)
  • 투고 : 2012.10.02
  • 심사 : 2012.11.17
  • 발행 : 2012.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Notch signaling plays fundamental roles in various animal development. It has been suggested that Hr-Notch, a Notch homologue in the ascidian Halocynthia roretzi, is involved in the formation of peripheral neurons by suppressing the neural fates and promoting the epidermal differentiation. However, roles of Notch signaling remain controversial in the formation of nervous system in ascidian embryos. To precisely investigate functions of Notch signaling, we have isolated and characterized Hr-Numb, a Numb homologue which is a negative regulator of Notch signaling, in H. roretzi. Maternal expression of Hr-Numb mRNAs was detected in egg cytoplasm and the transcripts were inherited by the animal blastomeres. Its zygotic expression became evident by the early neurula stage and the transcripts were detected in dorsal neural precursor cells. Suppression of Hr-Numb function by an antisense morpholino oligonucleotide resulted in larvae with defect in brain vesicle and palps formation. Similar results have been obtained by overexpression of the constitutively activated Hr-Notch forms. Therefore, these results suggest that Hr-Numb is involved in Notch signaling during ascidian embryogenesis.

키워드

참고문헌

  1. Akanuma T, Hori S, Darras S, Nishida H (2002) Notch signaling is involved in nervous system formation in ascidian embryos. Dev Genes Evol 212:459-472. https://doi.org/10.1007/s00427-002-0264-x
  2. Artavanis-Tsakonas S, Muskavitch MA (2010) Notch: the past, the present, and the future. Curr Top Dev Biol 92:1-29.
  3. Berdnik D, Torok T, Gonzalez-Gaitan M, Knoblich JA (2002) The endocytic protein alpha-Adaptin is required for numb-mediated asymmetric cell division in Drosophila. Dev Cell 3:221-231. https://doi.org/10.1016/S1534-5807(02)00215-0
  4. Bresciani E, Confalonieri S, Cermenati S, Cimbro S, Foglia E, Beltrame M, Di Fiore PP, Cotelli F (2010) Zebrafish numb and numblike are involved in primitive erythrocyte differentiation. PLoS One 5:e14296. https://doi.org/10.1371/journal.pone.0014296
  5. Campos-Ortega JA (1995) Genetic mechanisms of early neurogenesis in Drosophila melanogaster. Mol Neurobiol 10:75-89. https://doi.org/10.1007/BF02740668
  6. Chapman G, Liu L, Sahlgren C, Dahlqvist C, Lendahl U (2006) High levels of Notch signaling down-regulate Numb and Numblike. J Cell Biol 175:535-540. https://doi.org/10.1083/jcb.200602009
  7. Couturier L, Vodovar N, Schweisguth F (2012) Endocytosis by Numb breaks Notch symmetry at cytokinesis. Nat Cell Biol 14:131-139. https://doi.org/10.1038/ncb2419
  8. Guo M, Jan LY, Jan YN (1996) Control of daughter cell fates during asymmetric division: interaction of Numb and Notch. Neuron 17:27-41. https://doi.org/10.1016/S0896-6273(00)80278-0
  9. Hori S, Saitoh T, Matsumoto M, Makabe KW, Nishida H (1997) Notch homologue from Halocynthia roretzi is preferentially expressed in the central nervous system during ascidian embryogenesis. Dev Genes Evol 207:371-380. https://doi.org/10.1007/s004270050126
  10. Kandachar V, Roegiers F (2012) Endocytosis and control of Notch signaling. Curr Opin Cell Biol 24:534-540. https://doi.org/10.1016/j.ceb.2012.06.006
  11. Kim GJ, Kumano G, Nishida H (2007) Cell fate polarization in ascidian mesenchyme/muscle precursors by directed FGF signaling and role for an additional ectodermal FGF antagonizing signal in notochord/nerve cord precursors. Development 134:1509-1518. https://doi.org/10.1242/dev.02825
  12. Lai EC (2004) Notch signaling: control of cell communication and cell fate. Development 131:965-973. https://doi.org/10.1242/dev.01074
  13. Latimer AJ, Appel B (2006) Notch signaling regulates midline cell specification and proliferation in zebrafish. Dev Biol 298:392-402. https://doi.org/10.1016/j.ydbio.2006.05.039
  14. Lee WY, Ham HS, Kim GJ (2011) Expression of Wee1 gene in the ascidian, Halocynthia roretzi embryo. Dev Reprod 15:1-7.
  15. Meinertzhagen IA, Lemaire P, Okamura Y (2004) The neurobiology of the ascidian tadpole larva: recent developments in an ancient chordate. Annu Rev Neurosci 27:453-485. https://doi.org/10.1146/annurev.neuro.27.070203.144255
  16. Miya T, Morita K, Suzuki A, Ueno N, Satoh N (1997) Functional analysis of an ascidian homologue of vertebrate Bmp-2/Bmp-4 suggests its role in the inhibition of neural fate specification. Development 124:5149-5159.
  17. Niikura Y, Tabata Y, Tajima A, Inoue I, Arai K, Watanabe S (2006) Zebrafish Numb homologue: phylogenetic evolution and involvement in regulation of left-right asymmetry. Mech Dev 123:407-414. https://doi.org/10.1016/j.mod.2006.03.008
  18. Reugels AM, Boggetti B, Scheer N, Campos-Ortega JA (2006) Asymmetric localization of Numb: EGFP in dividing neuroepithelial cells during neurulation in Danio rerio. Dev Dyn 235:934-948. https://doi.org/10.1002/dvdy.20699
  19. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406-425.
  20. Santolini E, Puri C, Salcini AE, Gagliani MC, Pelicci PG, Tacchetti C, Di Fiore PP (2000) Numb is an endocytic protein. J Cell Biol 151:1345-1352. https://doi.org/10.1083/jcb.151.6.1345
  21. Stolfi A, Wagner E, Taliaferro JM, Chou S, Levine M (2011) Neural tube patterning by Ephrin, FGF and Notch signaling relays. Development 138:5429-5439. https://doi.org/10.1242/dev.072108
  22. Uemura T, Shepherd S, Ackerman L, Jan LY, Jan YN (1989) Numb, a gene required in determination of cell fate during sensory organ formation in Drosophila embryos. Cell 58:349-360. https://doi.org/10.1016/0092-8674(89)90849-0
  23. Zhong W, Jiang MM, Weinmaster G, Jan LY, Jan YN (1997) Differential expression of mammalian Numb, Numblike and Notch1 suggests distinct roles during mouse cortical neurogenesis. Development 124:1887-1897.