Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Dual Effectiveness of Opuntia ficus indica Extracts for Enzymatic Browning Inhibition and Microbial Inactivation on Fresh-cut Apples

백년초 추출물 처리가 신선절단 사과의 갈변억제와 Staphylococcus aureus 증식에 미치는 영향

  • Seo, Young-Ho (Seoul Metropolitan Government Research institute of Public Health and Environment)
  • Received : 2012.09.11
  • Accepted : 2012.11.13
  • Published : 2012.12.31
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Abstract

The dual effectiveness of Opuntia ficus indica extracts for browning inhibition and microbial inactivation on fresh-cut apples was investigated. Prepared apple slices were treated with 25, 50, 100, 200 mg/mL Opuntia ficus indica extracts, packaged in polyethylene bags, and stored for 10 days at 4, $21^{\circ}C$. Results indicate that Opuntia ficus indica extracts significantly (P < 0.05) inhibited the browning reaction of fresh-cut apples. This treatment also reduced peroxidase activities. The populations of Staphylococcus aureus significantly decreased with increasing extract concentration (p < 0.05). In particular, S. aureus was reduced to non-detectable levels after 2 days in 100 mg/mL treatment at $4^{\circ}C$ and $21^{\circ}C$. Opuntia ficus indica extracts therefore have antibacterial and antibrowning effects. The results suggest that Opuntia ficus indica extracts could be useful as a natural food preservative.

천연 갈변 및 미생물 억제제 소재를 발굴하기 위해 신선절단 사과에 백년초추출물을 처리하여 색도변화, peroxidase 활성, 황색포도상구균 생육을 조사하였다. 신선절단 사과의 L값은 백련초 추출물 처리구가 대조구에 비해 유의적으로 감소폭이 적어 갈변억제가 나타난 것을 확인할 수 있었으며, a값은 저장기간이 경과함에 따라 증가하였으며 백련초 추출물 처리구는 2일째부터 갈변억제효과를 보였다. Peroxidase 활성도 백년초 추출물 처리구에서 저장기간이 경과함에 따라 갈변억제효과를 확인하였다. 신선절단 사과에서 백년초 추출물 25 mg/mL 이상 처리구에서 유의적으로 황색포도상구균 생육억제 효과가 나타났으며, 온도별로는 $21^{\circ}C$ 저장시 억제효과가 더 큰 것으로 판단되었다. 특히 100 mg/mL 처리구에서 4일째부터 황색포도상구균이 검출한계 이하로 관찰되어 생육억제에 매우 효과적임을 확인 할 수 있었다. 따라서 본 연구결과, 백년초 추출물 처리는 신선절단 사과의 저장 중에 갈변억제 및 황색포도상구균의 생육억제에 효과적인 천연물이며, 향후 신선절단식품의 품질유지에 효과적인 식품첨가제로서 개발 가능하리라 판단된다.

Keywords

References

  1. Allende, A.F., Tomas, B. and Gil, MI.: Minimal processing for healthy traditional foods. Trends Food Sci. Tech., 17, 513-519 (2006). https://doi.org/10.1016/j.tifs.2006.04.005
  2. Watata, A.E., Abe, K. and Yanauchi, N.: Physiological activities of partially processed fruits and vegetables. Food Technol., 44, 116-122 (1990).
  3. Brecht, J.K.: Physiology of lightly processed fruits and vegetables. HortScience, 30, 18-22 (1995).
  4. Saftner, R.A., Abbott, J., Bhagwat, A.A. and Vinyard, B.: Quality measurement of intact and fresh-cut slices of Fuji, Granny Smith, Pink Lady and GoldRush apples. J. Food Sci., 70, 317-324 (2005). https://doi.org/10.1111/j.1365-2621.2005.tb11474.x
  5. Rupasinghe, H.P.V., Murr, D.P., DeEll, J.R. and Odumeru, J.: Effect of 1-methylcyclopropene(1-MCP) and NatureSealTM on the quality of fresh-cut apples. J. Food Quality, 28, 289-307 (2005). https://doi.org/10.1111/j.1745-4557.2005.00035.x
  6. Soliva-Fortuny, R.C. and Martin-Belloso, O.: New advances in extending the shelf-life of fresh-cut fruits. Trends Food Sci. Technol., 14, 341-353 (2003). https://doi.org/10.1016/S0924-2244(03)00054-2
  7. Seo, Y.H., Jang, J.H. and Moon, K.D.: Occurrence and characterization of enterotoxigenic Staphylococcus aureus Isolated from minimally processed vegetables and sprouts in Korea. Food Sci. Biotechnol., 19, 313-319 (2010). https://doi.org/10.1007/s10068-010-0045-7
  8. Lee, C.B.: Korean flora. Hyangmun Publishing Co., Seoul, pp. 559-565 (1985).
  9. Chung, H.J.: Antioxidative and antimicrobial activities of Opuntia ficus-indica var. saboten. Korean J. Soc. Food Sci., 16, 160-166 (2000).
  10. Park, E.H. and Chun, M.J.: Wound healing activity of Opuntia ficus-indica. Fitoterapia, 72, 165-167 (2001). https://doi.org/10.1016/S0367-326X(00)00265-3
  11. Seo, Y.H., Han, C.H., Lee, J.M., Choi, S.M. and Moon, K.D.: Effects of Opuntia ficus indica extracts on inactivation of Escherichia coli O157:H7 and Listeria monocytogenes on fresh-cut apples. J. Korean Soc. Food Sci. Nutr., 41, 1009-1013 (2012). https://doi.org/10.3746/jkfn.2012.41.7.1009
  12. Cho, I.K., Seo, K.S. and Kim, Y.D.: Antimicrobial activity, antioxidant effects and total polyphenol contents of extracts of prickly pear, Opuntia ficus indica. Korean J. Food Preserv., 16, 953-958 (2009).
  13. Galati, E.M., Tripodo, M.M., Trovato, A., Miceli, N. and Monforte, M.T.: Biological effect of Opuntia ficus indica(L.) Mill. waste matter Note I: diuretic activity. J. Ethnopharmacol, 79, 17-21 (2002). https://doi.org/10.1016/S0378-8741(01)00337-3
  14. Gurrieri, S., Miceli, L., Lanza, C.M., Toomaselli, F., Bonomo, R.P. and Rizzarelli, E.: Chemical characterization of Sicilian prickly pear (Opuntia ficus-indica) and perspectives for the storage of its juice. J. Agric. Food Chem., 48, 5424-5431 (2000). https://doi.org/10.1021/jf9907844
  15. Aquino-Bolans, E.H. and Mercado-Silva, E.: Effects of polyphenol oxidase and peroxidase activity, phenolics and lignin content on the browning of cut jicama. Postharvest Biol. Tech., 33, 275-283 (2004). https://doi.org/10.1016/j.postharvbio.2004.03.009
  16. Zocca, A. and Ryugo, K.: Changes in polyphenoloxidase activity and substrate levels in maturing Golden Delicious apple and other cultivars. HortScience, 10, 586-587 (1975).
  17. Weller, A., Sims, C.A., Matthews, R.F., Bates, R.P. and Brecht J.K.: Browning susceptibility and changes in composition during storage of carambola silces. J. Food Sci., 62, 256-260 (1997). https://doi.org/10.1111/j.1365-2621.1997.tb03980.x
  18. Gomez-Lopez, V.M., Devlieghere, F., Ragaert, P. and Debevere, J.: Shelf-life extension of minimally processed carrots by gaseous chlorine dioxide. Int. J. Food Microbiol., 116, 221-227 (2007). https://doi.org/10.1016/j.ijfoodmicro.2006.12.008
  19. Wu, V.C., Qiu, X., Bushway, A. and Harper, L.: Antibacterial effects of American cranberry (Vaccinium macrocarpon) concentrate on foodborne pathogens. Food Sci. Tech., 41, 1834-1841 (2008).
  20. Jung, E.J., Hong, S.J., Choi, J.E., Jeong, S.S., Oh, H.N., Lee, H.J. and Choi, C.H.: In vitro growth inhibition of Streptococcus mutans by extract of prickly pear (Opuntia ficus-indica var. saboten Makino). J. Korean Academy of Oral Health, 34, 28-35 (2010).