대한의생명과학회지 (Biomedical Science Letters)

  • 제18권4호
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  • Pages.362-368
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  • 2012
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  • 1738-3226(pISSN)
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  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
권호 표지

Evaluation of Enterotoxigenic Bacteroides fragilis from Colonic Washings from Patients Undergoing Colonoscopy

  • Van, Ni (Department of Medicine, Section of Digestive Diseases & Nutrition, University of Illinois at Chicago) ;
  • Ahlberg, Ned (Department of Medicine, Section of Digestive Diseases & Nutrition, University of Illinois at Chicago) ;
  • Jung, Byung Chul (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Lee, Min Ho (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Ahn, Seung Ju (Department of Biomedical Laboratory Science, Daegu Health College) ;
  • Lee, In-Soo (Department of Clinical Laboratory Science, Hyejeon College) ;
  • Kim, Yoon Suk (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Rhee, Ki-Jong (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
  • 투고 : 2012.09.26
  • 심사 : 2012.10.04
  • 발행 : 2012.12.31
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초록

Enterotoxigenic Bacteroides fragilis (ETBF) is an intestinal commensal bacterium implicated as a risk factor for colon cancer. The key virulence factor is a secreted toxin called B. fragilis toxin (BFT). In this study we used an in vitro bioassay to examine the prevalence of ETBF in colonic washings from patients with colorectal polyps and normal control patients. We found that 9.3% of polyp patients and 10.9% of non-polyp patients harbored ETBF, respectively. A total of nine ETBF clinical isolates were isolated and confirmed to be positive for the BFT gene by PCR analysis and the ability to induce IL-8 secretion in the colonic epithelial cell line HT29/c1. Two of the ETBF clinical strains were characterized further in vitro and in vivo. We found that the two ETBF clinical isolates induced E-cadherin cleavage in HT29/c1 cells and promoted colonic inflammation in C57BL/6 mice. Our results indicate that the prevalence of ETBF in polyp patients were similar in non-polyp patients suggesting that ETBF carriage does not positively correlate to polyp incidence.

키워드

참고문헌

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