Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Effects of Beta-glucan from Coriolus versicolor on Scavenger Receptor B1 Expression and their Involvement of Dectin-1 and Casein Kinase 2

  • Kim, Taeseong (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Kim, Ye-Jin (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Sohn, Eun-Hwa (Department of Herbal Medicine Resource, Kangwon National University)
  • Received : 2012.11.21
  • Accepted : 2012.12.06
  • Published : 2012.12.31
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Abstract

The mushroom Coriolusversicolor contains biologically active polysaccharides, most of which belong to the ${\beta}$ glucan group. Diverse physicochemical properties, due to different sources and isolated types of ${\beta}$-glucans, may induce distinct biological activities. Here, we examined the effects of ${\beta}$-glucan from Coriolusversicolor (CVG) on the scavenger receptor B1 (SR-B1) expression and the role of SR-B1 in CVG-induced phagocytosis regulation by using SR-B1-specific shRNA transfected cells. We also examined whether Dectin-1 and CK2 are involved in SR-B1 expression in CVG-treated cells. Our study results showed that CVG increased the SR-B1 expression via Dectin-1 and CK2 in macrophages. However, the inhibition of SR-B1 expression by shRNA did not completely eliminate the effect of CVG on the increase of phagocytosis suggesting that SR-B1 is not essential for CVG-stimulated phagocytosis. This study will contribute to identify CVG's mechanism of action and its use in the development of functional foods.

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References

  1. Brown, G.D., P.R. Taylor, D.M. Reid, J.A. Willment, D.L. Williams, L. Martinez-Pomares, S.Y. Wong and S. Gordon. 2002. Dectin-1 is a major $\beta$-glucan receptor on macrophages. J. Exp. Med. 196:407-412. https://doi.org/10.1084/jem.20020470
  2. Choung, M.G. and E.H. Sohn. 2011. Anti-tumor activity of saponin fraction of Platycodon gradiflourm through immunomodulatory effects associated with NO production in RAW264.7 cells. J. Korean Plant Res. 24(5): 557-563 (in Korean). https://doi.org/10.7732/kjpr.2011.24.5.557
  3. Duncan, J.S. and D.W. Litchfield. 2008. Too much of a good thing: the role of protein kinase CK2 in tumorigenesis and prospects for therapeutic inhibition of CK2. Biochim. Biophys. Acta. 1784(1):33-47. https://doi.org/10.1016/j.bbapap.2007.08.017
  4. Dushkin, M.I., A.F. Safina, E.I. Vereschagin and Y.S. Schwartz. 1996. Carboxymethylated $\beta$-1,3-glucan inhibits the binding and degradation of acetylated low density lipoproteins in macrophages in vitro and modulates their plasma clearance in vivo. Cell Biochem. Funct. 14:209-217.
  5. Gantner, B.N., R.M. Simmons, S.J. Canavera, S. Akira and D.M. Underhill. 2003. Collaborative induction of inflammatory responses by Dectin-1 and toll-like receptor. J. Exp. Med. 197:1107-1117. https://doi.org/10.1084/jem.20021787
  6. Greaves, D.R., P.J. Gough and S. Gordon. 1998. Recent progress in defining the role of scavenger receptors in lipid transport, atherosclerosis and host defence. Curr. Opin. Lipidol. 9: 425-932. https://doi.org/10.1097/00041433-199810000-00006
  7. Goodridge, H.S., C.N. Reyes, C.A. Becker, T.R. Katsumoto, J. Ma, A.J. Wolf, N. Bose, A.S. Chan, A.S. Magee, M.E. Danielson, A. Weiss, J.P. Vasilakos and D.M. Underhill. 2011. Activation of the innate immune receptor Dectin-1 upon formation of a 'phagocytic synapse'. Nature 472(7344): 471-475. https://doi.org/10.1038/nature10071
  8. Ho, C.Y., C.F. Kim, K.N. Leung, K.P. Fung, T.F. Tse, H. Chan and C.B. Lau. 2005. Differential anti-tumor activity of Coriolus versicolor (Yunzhi) extract through p53- and/or Bcl-2-dependent apoptotic pathway in human breast cancer cells. Cancer Biol. Ther. 4:638-644. https://doi.org/10.4161/cbt.4.6.1721
  9. Hollifield, M., E.B. Ghanem, W.J.S. de Villiers and B.A. Garvy. 2007. Scavenger receptor A dampens induction of inflammation in response to the fungal pathogen Pneumocystis carinii. Infect. Immun. 75:3999-4005. https://doi.org/10.1128/IAI.00393-07
  10. Itoh, W., I. Sugawara, S. Kimura, K. Tabata, A. Hirata, T. Kojima, S. Mori and K. Shuimada. 1990. Immunopharmacological study of sulfated schizophyllan (SPG) I. Its action as a mitogen and anti-HIV agent. Int. J. Immunopharmacol. 12:225-233. https://doi.org/10.1016/0192-0561(90)90057-T
  11. Janeway, C.A. and R. Medzhitov. 2002. Innate immune recognition. Annu. Rev. Immunol. 20:197-216. https://doi.org/10.1146/annurev.immunol.20.083001.084359
  12. Jang, S.A., S. Namkoong and E.H. Sohn. 2010. Effects of $\beta$ -glucans from Coriolus versicolor on macrophage functions in young and aged mice J. Korean Plant Res. 23(6):498-502.
  13. Jang, S.A., S. Park, J.D. Lim, C.H. Kang, K.H. Yang, S. Pyo and E.H. Sohn. 2009. The comparative immunomodulatory effects of$\beta$$\beta$-glucans from yeast, bacteria, and mushroom on the function of macrophages. J. Food Sci. Nutr. 14:102-108. https://doi.org/10.3746/jfn.2009.14.2.102
  14. Kidd, P.M. 2000. The use of mushroom glucans and proteoglycans in cancer treatment. Altern. Med. Rev. 5:4-27.
  15. Muller, A., P.J. Rice, H.E. Ensley, P.S. Coogan, J.H. Kalbfleisch, J.L. Kelley, E.J. Love, C.A. Portera, T. Ha, I.W. Browder and D.L. Williams. 1996. Receptor binding and internalization of water-soluble (1$\rightarrow$3)-$\beta$-D-glucan biologic response modifier in two monocyte/macrophage cell lines. J. Immunol. 156: 3418-3425.
  16. Okimura, T., M. Ogawa and T. Yamauchi. 1986. Stress and immune responses. III. Effect of resistant stress on delayed type hypersensitivity (DTH) response, natural killer (NK) activity and phagocytosis in mice. Jpn. J. Pharmacol. 41: 229-235. https://doi.org/10.1254/jjp.41.229
  17. Peiser, L., S. Mukhopadhyay and S. Gordon. 2002. Scavenger receptors in innate immunity. Current Opinion in Immunology 14:123-128. https://doi.org/10.1016/S0952-7915(01)00307-7
  18. Ross, G.D. 2000. Regulation of the adhesion versus cytotoxic functions of the Mac-1/CR3/alphaMbeta2-integrin glycoprotein. Crit. Rev. Immunol. 20:197-222.
  19. Taylor, P.R., S.V. Tsoni, J.A. Willment, K.M. Dennehy, M. Rosas, H. Findon, K. Haynes, C. Steele, M. Botto, S. Gordon and G.D. Brown. 2007. Dectin-1 is required for $\beta$-glucan recognition and control of fungal infection. Nat. Immunol. 8:31-38. https://doi.org/10.1038/ni1408
  20. Unger, G.M., A.T. Davis, J.W. Slaton and K. Ahmed. 2004. Protein kinase CK2 as regulator of cell survival: implications for cancer therapy. Curr. Cancer Drug Targets 4(1):77-84. https://doi.org/10.2174/1568009043481687
  21. Vereschagin, E.I., A.A. Van Lambalgen, M.I. Dushkin, Y.S. Schwartz, L. Polyakov, A. Heemskerk, E. Huisman, L.G. Thijs and G.C. Van den Bos. 1998. Soluble glucan protects against endotoxin shock in the rat: the role of the scavenger receptor. Shock 9:193-198. https://doi.org/10.1097/00024382-199803000-00006
  22. Williams, D.L., H.A. Pretus and I.W. Browder. 1992. Application of aqueous gel permeation chromatography with in-line multi-angle laser light scattering and differential viscometry detectors for the characterization of natural product carbohydrate pharmaceuticals. J. Liq. Chromatogr. 15:2297-2309. https://doi.org/10.1080/10826079208016179
  23. Willment, J.A., A.S.J. Marshall, D.M. Reid, D. L. Williams, S.Y. Wong, S. Gordon and G.D. Brown. 2005. The human $\beta$ -glucan receptor is widely expressed and functionally equivalent to murine Dectin-1 on primary cells. Eur. J. Immunol. 35: 1539-1547. https://doi.org/10.1002/eji.200425725
  24. Yuhanna, I.S., Y. Zhu, B.E. Cox, L.D. Hahner, S. Osborne- Lawrence, P. Lu, Y.L. Marcel, R.G. Anderson, M.E. Mendelsohn, H.H. Hobbs and P.W. Shaul. 2001. High-density lipoprotein binding to scavenger receptor-B1 activates endothelial nitric oxide synthase. Nat. Med. 7:853-857. https://doi.org/10.1038/89986