Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Nematicidal Effect of Root-Knot Nematode (Meloidogyne incognita) by Amino Acids Biochemical Agent Extracted from Chicken Feather

닭 우모로부터 추출한 아미노산 생화학제의 고구마뿌리혹선충 증식억제 효과

  • Kim, Se-Jong (Department of Microbial & Nano Materials, Mokwon University) ;
  • Whang, Kyung-Sook (Department of Microbial & Nano Materials, Mokwon University/Institute of Microbial Ecology and Resources, Mokwon University)
  • Received : 2012.08.27
  • Accepted : 2012.09.13
  • Published : 2012.12.31
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Abstract

This study examined the control effects of amino acid biochemical agent extracted from chicken feathers on Meloidogyne incognita for the purpose of developing an environmentally friendly nematicidal agent that can replace chemical control of root-knot nematodes (RKN). We investigated the lethal effects of J2 juveniles for 19 types of commercial amino acids. As a result, five kinds of amino acids (L-asparagine, L-aspartic acid, L-methionine, L-tyrosine, L-cysteine) showed mortality rate of more than 50% at a concentration of 50 mM. L-asparagine showed the highest mortality rate at 94%. We also investigated the lethal effect of J2 juveniles and suppressive effects of egg hatching by feather amino acids (FAA) biochemical agent. It showed that the mortality rate of J2 juveniles was more than 80% and suppression rate of egg hatching was 74% at 1/50 concentrations of FAA. As a result of conducting a tomato pot culture experiment for 60 days after treating 1/50 concentrations of FAA biochemical agent in rhizosphere soil, it showed that the control effects were 63% of juveniles density in the soil, 59% of egg mass and 61% of root gall index, respectively. Based on the above results, it is considered that the FAA biochemical agent extracted from chicken feathers can be used as an environmentally friendly nematicidal agent of RKN.

본 연구는 뿌리혹선충의 화학적 방제를 대체할 수 있는 친환경 선충방제제 개발을 목적으로 닭 우모분으로부터 추출한 아미노산 생화학제(feather amino acid; FAA)의 고구마뿌리혹선충에 대한 방제효과를 검정하였다. 다양한 종류의 아미노산을 대상으로 고구마뿌리혹선충 유충 치사효과를 조사한 결과, 5종류(L-asparagine, L-aspartic acid, L-methionine, L-tyrosine, _L-cysteine)의 아미노산이 50 mM 농도에서 50% 이상의 치사율을 나타내었고, L-asparagine 50 mM에서 치사율이 94%로 가장 높았다. 닭 우모분으로부터 추출한 FAA 생화학제의 고구마뿌리혹선충 2령 유충의 치사력과 알 부화억제 효과를 조사한 결과, 1/50 농도에서 80% 이상의 유충 치사력과 74%의 알 부화 억제율을 보였다. 토마토 근권토양에 FAA 생화학제를 처리하고 60일간 재배시험을 통하여 관찰한 결과, 토양 내 유충밀도는 63%, 난낭수는 59%, 뿌리혹 형성 61%의 방제효과를 나타내었다. 이상의 결과로부터 닭 우모분으로부터 추출한 FAA 생화학제는 뿌리혹선충의 친환경 선충방제제로 활용될 수 있을 것으로 사료된다.

Keywords

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