대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
권호 표지

담관암세포주에서 지유(地楡) 에탄올 추출물의 항암 효과

Pro-apoptotic Effects of Sanguisorbae Radix Ethanol-Extracts on Two Cholangiocarcinoma Cell Lines, SNU-1079 and SNU-1196

  • 박진규 (경희대학교 대학원 한의학과 간계내과학교실) ;
  • 김영철 (경희대학교 대학원 한의학과 간계내과학교실)
  • Park, Jin-Kyu (Dept. of Internal Medicine, Oriental Medicine Graduate School, Kyung-Hee University) ;
  • Kim, Young-Chul (Dept. of Internal Medicine, Oriental Medicine Graduate School, Kyung-Hee University)
  • 발행 : 2012.12.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives : This study was performed to investigate the antineoplastic effect of ethanol extracts from Sanguisorbae Radix on cholangiocarcinoma cells that was established from biliary tract cancer tissue. Materials and Methods : Two cholangiocarcinoma cell lines, SNU-1079 and SNU-1196, were studied. The mRNA expression of Caspase 3, 8, 9, Bcl-2, Bax, P53, and P21 was examined by RT-PCR. Cell viability was determined by MTT assay. The cell cycle was analyzed by flow cytometry and apoptosis by cell death detection ELISA kit. Results : Proliferation of SNU-1079 and SNU-1196 was inhibited by Sanguisorbae Radix treatment in a dose-dependent manner. All cells treated with Sanguisorbae Radix showed increased dose- and time-dependent apoptosis. The expression of caspase 3, 8, 9, p53, and p21 was increased in all cells after the treatment of Sanguisorbae Radix. The expression of Bcl-2 was decreased in SNU-1196 and Bax expression was increased in all cells after the treatment of Sanguisorbae Radix. Conclusions : These results suggest that Sanguisorbae Radix would be beneficial in the treatment of cholangiocarcinoma.

키워드

참고문헌

  1. Patel T. Increasing incidence and mortality of primary intrahepatic cholnagiocarcinoma in the United States. Hepatology 2001;33:1353-7. https://doi.org/10.1053/jhep.2001.25087
  2. Davila JA, El-serag HB. Cholangiocarcinoma: the "other"liver cancer on the rise. Am J Gastroenterol 2002;97:3199-200.
  3. 보건복지부 홈페이지:2012.[2012.06.27.검색]. Available at: http://kosis.kr/gen_etl/start.jsp?orgId=117&tblId =DT_117N_A0024&conn_path=I2&path= 중앙행정기관 - 보건복지부 - 암등록통계 - 61개 암종/성/연령(5세)별 암발생자수.
  4. Aljiffry M, Walsh MJ, Molinari M. Advances in diagnosis, treatment and palliation of cholangiocarcinoma: 1990-2009. World J Gastroenterol 2009;15:4240-62. https://doi.org/10.3748/wjg.15.4240
  5. Thongprasert S. The role of chemotherapy in cholangiocarcinoma. Ann Oncol 2005;16 Suppl 2 :ii93-6. https://doi.org/10.1093/annonc/mdi712
  6. Itoh H, Nishijima K, Kurosaka Y, Takegawa S, Kiriyama M, Dohba S, et al. Magnitude of combination therapy of radical resection and external beam radiotherapy for patients with carcinomas of the extrahepatic bile duct and gallbladder. Dig Dis Sci 2005 Dec;50(12):2231-42.
  7. Gruenberger B, Schueller J, Tamandl D, Kaczirek K, Schernthaner G, Gruenberger T. K-ras status and response in patients with advanced or metastatic cholangiocarcinoma treated with cetuximab plus gemcitabine-oxaliplatin (GEMOX): a single center phase II study. J Clin Oncol 2009;27(Suppl 15):4586, [Abstract].
  8. 전국한의과대학 공동교재편찬위원회. 본초학. 서울: 영림사; 2004, p. 437-8.
  9. 안봉전, 이진태, 이순애, 곽재훈, 박정미, 이진영,등. 한국산 地楡의 항산화 효과 및 천연소재로서의 활용방안. 한국응용생명화학회지 2004;47(2) :244-50.
  10. 안봉전, 이순애, 손준호, 곽재훈, 박정미, 이진영. 地楡의 항암 및 항균 효과. 한국응용생명화학회지 2004;47(1):141-5.
  11. 이소영, 정성현. 地楡의 항 불안 활성. 대한약학회지 1994;38(6):733-41.
  12. 이재준. 최현숙, 이정화, 정창주, 이명렬. 地楡가 흰쥐의 급성위염 및 소화성 궤양에 미치는 영향. 한국식품영양학회지 2005;34(10):1545-52.
  13. 지형준 외. 대한약전 및 대한약전외 한약규격주해. 서울: 한국메디칼인덱스사; 1998.
  14. Hammill CW, Wong LL. Intrahepatic cholangiocarcinoma: a malignancy of increasing importance. J Am Coll Surg 2008;207:594-603. https://doi.org/10.1016/j.jamcollsurg.2008.04.031
  15. Nathan H, Pawlik TM, Wolfgang CL, Choti MA, Cameron JL, Schulick RD. Trends in survival after surgery for cholangiocarcinoma: a 30-year population-based SEER database analysis. J Gastrointest Surg 2007;11:1488-96. https://doi.org/10.1007/s11605-007-0282-0
  16. Song GW, Lee SG, Lee YJ, Park KM, Hwang S, Kim KH, et al. Analysis of survival and factors affecting the survival after surgical resection of peripheral cholangiocarcinoma: 318 case in single institute. Korean J Hepatol 2007; 13:208-21.
  17. Hanahan D, Weinberg RA. The halmarks of cancer. Cell. 2000;100(1):57-70. https://doi.org/10.1016/S0092-8674(00)81683-9
  18. Sirica AE. Cholangiocarcinoma: molecular targeting strategies for chemoprevention and therapy. Hepatology 2005;41:5-15. https://doi.org/10.1002/hep.20537
  19. Blechacz B, Gores GJ. Cholangiocarcinoma: advances in pathogenesis, diagnosis, and treatment. Hepatology 2008;48:308-21.
  20. 김동우, 이종훈, 유화승, 조정효, 이연월, 손창규, 등. 천화분 추출물이 혈관신생 및 암세포 성장에 미치는 영향. 대한한방내과학회지 2008; 29(2):490-9.
  21. 박병록, 박재우, 조종관, 유화승, 이연월. 한방치료를 통해 Adriamycin/Cytoxan 및 Taxol 항암제 부작용이 감소한 유방암 환자 증례보고. 대한한방내과학회지 2011;32(3):451-7.
  22. 문근아, 송낙근, 박성진, 윤유식. 가미홍화탕의 방사선 부작용 억제 효능 연구. 한국한의학연구원논문집 2003;9(2):107-19.
  23. 서영광, 김은희, 김달래, 고병희, 전성하, 어완규, 등. 폐전이된 담낭암 환자를 사상의학적 관리를 통해 제반 증상과 삶의 질을 개선시킨 1례. 사상체질의학회지 2007;19(2):187-94.
  24. 김경순, 유화승, 정태영, 이연월, 조종관. 항암단 투여로 호전된 진행성 또는 전이성 담낭암 연속 환자증례. 대한한방내과학회지 2010;31(3):667-74.
  25. 송창훈, 이슬희, 김정철, 오성원, 정종진, 황진우, 등. 원위부 담도암환자 증례 보고. 대한한방내과학회지 2006;27(3):770-5.
  26. 서상훈, 유화승, 이용연, 송기철, 최병렬, 조정효, 등. 췌장전이를 동반한 담도암 환자 1례에 대한 보고. 대한한방내과학회지 2002;23(4):735-40.
  27. 정성훈, 김병호, 김영일, 심재준, 황보영, 장재영, 등. 담관암세포주에서 내인성peroxisome proliferator -activated receptor gamma 배위자인 15-deoxy-delta (12,14)-prostaglandin J2의 항암 효과. 대한내과학회지 2010;78(1):75-86.
  28. Iwata M, Harada K, Kono N, Kaneko S, Kobayashi K, Nakanuma Y. Expression of Bcl-2 familial proteins is reduced in small bile duct lesions of primary biliary cirrhosis. Hum Pathol 2000;31:179-84. https://doi.org/10.1016/S0046-8177(00)80217-8
  29. Harada K, Iwata M, Kono N, Koda W, Shimonishi T, Nakanuma Y. Distribution of apoptotic cells and expression of apoptosisrelated proteins along the intrahepatic biliary tree in normal and non-biliary diseased liver.Histopathology 2000;37:347-54. https://doi.org/10.1046/j.1365-2559.2000.00982.x
  30. Ito Y, Takeda T, Sasaki Y, Sakon M, Monden M, Yamada T, et al. Bcl-2 expression in cholangiocarcinoma is inversely correlated with biologically aggressive phenotypes. Oncology 2000;59:63-7. https://doi.org/10.1159/000012139