대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
권호 표지

오적산의 추출 용매 차이에 따른 염증 및 염증 관련 질환에 대한 효능 비교

Effects of water and ethanol extracts from Ojeok-san on inflammation and its related diseases

  • 유새롬 (한국한의학연구원 한약기초연구그룹) ;
  • 정수진 (한국한의학연구원 한약기초연구그룹) ;
  • 김예지 (한국한의학연구원 한약기초연구그룹) ;
  • 임혜선 (한국한의학연구원 한약기초연구그룹) ;
  • 진성은 (한국한의학연구원 한약기초연구그룹) ;
  • 전우영 (한국한의학연구원 한약기초연구그룹) ;
  • 신인식 (한국한의학연구원 한약기초연구그룹) ;
  • 신나라 (한국한의학연구원 한약기초연구그룹) ;
  • 김성실 (한국한의학연구원 한약기초연구그룹) ;
  • 김정훈 (한국한의학연구원 한약기초연구그룹) ;
  • 하혜경 (한국한의학연구원 한약기초연구그룹) ;
  • 이미영 (한국한의학연구원 한약기초연구그룹) ;
  • 김온순 (한국한의학연구원 한약기초연구그룹) ;
  • 서창섭 (한국한의학연구원 한약기초연구그룹) ;
  • 신현규 (한국한의학연구원 한약기초연구그룹)
  • Yoo, Sae-Rom (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Jeong, Soo-Jin (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Ye-Ji (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Lim, Hye-Sun (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Jin, Seong-Eun (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Jeon, Woo-Young (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Shin, In-Sik (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Shin, Na-Ra (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Seong-Sil (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Jung-Hoon (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyung (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Lee, Mee-Young (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Kim, Ohn-Soon (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Seo, Chang-Seob (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
  • 발행 : 2012.12.30
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초록

Objectives : Ojeok-san, a traditional herbal formula, has been used for the treatment of cold illness and its related symptoms such as headache, nausea and indigestion. This study was performed to compare effects of water (OJSW) and 70% ethanol extracts (OJSE) of Ojeok-san on inflammation and its related diseases atopy, asthma and obesity in vitro. Methods : We performed HPLC to investigate contents of index components of OJSW and OJSE. We investigated the effects of OJSW and OJSE with an in vitro model, using 5 cell lines, specifically RAW 264. 7, HaCaT, MC/9, BEAS-2B and 3T3-L1. Results : HPLC analysis displayed that the contents of index components were higher in OJSE than OJSW. In lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, OJSE significantly inhibited productions of interleukin (IL)-6, nitrite and prostaglandin $E_2$ ($PEG_2$). In TNF-${\alpha}$/IFN-${\gamma}$-treated HaCaT keratinocytes, OJSE significantly lowered levels of macrophage-derived chemokine (MDC) as well as regulated and normal T cell expressed and secreted (RANTES). OJSE also had a protective effect on inflammatory response by decreasing RANTES secretion in TNF-${\alpha}$-stimulated BEAS-2B cells. Conclusions : We conclude that OJSE could be more appropriate to enhance the biological activities against inflammation and its related diseases, and could be applied as a bioactive material for developing the potent anti-inflammatory agents.

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