The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Effect of Lindera obtusiloba extract on cancer metastasis

생강나무 추출물의 암전이 억제효과

  • Yun, Hyuk (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Lee, Yong-Jae (College of Pharmacy, Woo-Suk University) ;
  • Seo, Hyun-Won (College of Pharmacy, Woo-Suk University) ;
  • Park, Kyoung-Jae (College of Pharmacy, Woo-Suk University) ;
  • Ko, Ha-Neul (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University) ;
  • Cha, Dong-Seok (College of Pharmacy, Woo-Suk University) ;
  • Kwon, Jin (Dept. of Prosthetics and Orthotics, Korea National College of Rehabilitation and Welfare) ;
  • Jeon, Hoon (College of Pharmacy, Woo-Suk University) ;
  • Kim, Kang-San (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University)
  • 윤혁 (원광대학교 한의과대학 내과학교실) ;
  • 이용재 (우석대학교 약학대학) ;
  • 서현원 (우석대학교 약학대학) ;
  • 박경재 (우석대학교 약학대학) ;
  • 고하늘 (원광대학교 한의과대학 내과학교실) ;
  • 차동석 (우석대학교 약학대학) ;
  • 권진 (한국재활복지대학 의료보장구과) ;
  • 전훈 (우석대학교 약학대학) ;
  • 김강산 (원광대학교 한의과대학 내과학교실)
  • Published : 2012.12.30
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Abstract

Objectives : In the present study, anti-metastatic properties of the methanol extract of L. obtusiloba (MLO) were evaluated. Methods : To determine the effect of MLO on cancer metastasis, we checked matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) activities and expressions in B16F10 melanoma cells. In addition, we performed cell migration assay as well as invasion assay using Matrigel. Finally, we used an in vivo lung metastasis model to confirm the anti-metastatic activity of MLO. Results : 1. MLO showed potent inhibitory effects on MMP-2 and MMP-9 activities and expressions via down-regulation of activation of NF-${\kappa}B$ in B16F10 melanoma cells. 2. Melanoma cell migration and invasion were down-regulated by MLO treatment. 3. Not only in vitro model, but MLO also significantly suppressed lung metastasis in vivo. Conclusions : The present results indicate that MLO has strong inhibitory effect on cancer metastasis. Therefore, L. obtusiloba could be a valuable anti-metastatic agent.

목 적 : 본 연구에서는 생강나무 메탄올 추출물이 암전이 억제에 미치는 영향을 조사하고자 하였다. 방 법 : 생강나무 추출물의 암전이 억제능을 확인하기 위해서 B16F10 흑색종 세포를 이용하여 금속단백분해효소의 활성 및 발현을 측정하였으며, 암세포의 이동능이나 침윤능도 조사하였다. 폐전이 동물모델에서 생강나무 추출물이 미치는 영향을 조사하여 활성을 최종적으로 확인하였다. 결 과 : 1. 생강나무 추출물은 B16F10 흑색종 세포에서 뚜렷한 금속단백분해효소의 효소활성 및 발현 억제효과를 보였으며 이는 NF-${\kappa}B$의 활성 억제에서 기인한 것임을 확인하였다. 2. 흑색종 세포의 이동이나 침윤 역시 생강나무 추출물 투여에 의해 현저히 감소하였다. 3. 폐전이 동물 모델에서도 생강나무 추출물에 의해 폐로 전이되 집락의 수가 감소하였다. 결 론 : 이상의 결과로 생강나무 추출물은 뛰어난 암전이 억제효과가 있는 것을 확인할 수 있었으며, 전이성 암치료에 있어서 유용하게 사용될 수 있을 것으로 사료된다.

Keywords

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