The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Nelumbinis Folium Methanol Extract Regulates Inducible Nitric Oxide Synthase Signaling Pathways in Mouse Peritoneal Macrophages

하엽(荷葉) 메탄올 추출물의 마우스 대식세포 inducible nitric oxide synthase 합성과 신호전달에 대한 조절

  • Lee, Si-Eun (Department of East-West Medical Science, Graduate School of East-West Medical Science, KyungHee University) ;
  • Lee, Mi-Hwa (Department of Herbology, College of Oriental Medicine, KyungHee University) ;
  • Choi, Ho-Young (Department of Herbology, College of Oriental Medicine, KyungHee University) ;
  • Sohn, Nak-Won (Department of East-West Medical Science, Graduate School of East-West Medical Science, KyungHee University) ;
  • Kang, Hee (Department of East-West Medical Science, Graduate School of East-West Medical Science, KyungHee University)
  • 이시은 (경희대학교 동서의학대학원 동서의과학과) ;
  • 이미화 (경희대학교 한의과대학 본초학교실) ;
  • 최호영 (경희대학교 한의과대학 본초학교실) ;
  • 손낙원 (경희대학교 동서의학대학원 동서의과학과) ;
  • 강희 (경희대학교 동서의학대학원 동서의과학과)
  • Received : 2011.12.14
  • Accepted : 2012.01.13
  • Published : 2012.01.30
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Abstract

Objective : Nelumbinis Folium (NF) is used for diarrhea, headache and dizziness in traditional medicine. In this paper, we examined the anti-inflammatory effects of the methanol extract of NF in mouse macrophages. Methods : Peritoneal macrophages from thioglycollate medium-injected mice were cultured and stimulated with lipopolysaccharide(LPS) or LPS/interferon(IFN)-${\gamma}$ for viability assay, cytokine measurement and Western blotting. Results : NF methanol extract suppressed the levels of nitric oxide (NO) through reduction of inducible NO synthase in a concentration-dependent manner. The extract reduced LPS/IFN-${\gamma}$-stimulated STAT1 phosphorylation and LPS-induced $I{\kappa}B{\alpha}$ degradation through inhibition of $I{\kappa}B{\alpha}$ kinase activation. The extract also inhibited p38, JNK/SAPK and ERK1/2 activation. Conclusions : Our findings suggested that NF has anti-inflammatory activity, and have a potential for therapeutic application. Further research is required to investigate its anti-inflammatory active compounds.

Keywords

References

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