한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제41권1호
  • /
  • Pages.20-25
  • /
  • 2012
  • /
  • 1226-3311(pISSN)
  • /
  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
권호 표지
DOI QR코드

DOI QR Code

천년초 줄기의 항균, 항산화 및 항암효과에 관한 연구

The Effects of Antimicrobial, Antioxidant, and Anticancer Properties of Opuntia humifusa Stems

  • 정복미 (전남대학교(여수) 영양식품학) ;
  • 신미옥 (양산대학교 커피바리스타제과과) ;
  • 김형락 (부경대학교 식품영양학과)
  • Jung, Bok-Mi (Major in Food Science and Nutrition, Chonnam National University) ;
  • Shin, Mi-Ok (Dept. of Coffee Barista Confectionery, Yangsan University) ;
  • Kim, Hyung-Rak (Dept. of Food Science and Nutrition, Pukyong National University)
  • 투고 : 2011.10.19
  • 심사 : 2011.12.26
  • 발행 : 2012.01.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 천년초 줄기를 이용하여 항균, 항산화, 항암 등 천년초의 생리활성 효과를 연구하였으며 그 결과는 다음과 같다. 천년초 줄기의 추출물중 gram 양성균인 4가지 균주(Rhodococcus equi, Staphylococcus aureus, Clostridium perfringens, Bacillus cereus)에서 항균 효과가 나타난 것은 메탄올과 핵산층이었으며, 4가지 균주 중 Bacillus cereus를 제외한 3종에 대해서는 우수한 항균효과를 나타냈다. 천년 초 줄기의 peroxynitrite($ONOO^-$) 제거능 측정결과 핵산층을 제외한 메탄올층, 부탄올층, 수층의 항산화 활성은 천년초 줄기 추출물의 농도가 증가할수록 항산화 효과가 유의적으로(p<0.05) 높게 나타났다. 천년초 줄기 추출물의 각 용매별 DPPH 유리기 소거능은 농도가 증가함에 따라 소거능도 유의적으로(p<0.05) 증가하였으며, 부탄올>메탄올>수층>핵산층 순으로 높게 나타났다. 천년초 줄기의 암세포 증식 억제 효과에서 피부암 세포의 경우 $50\sim100{\mu}g/mL$의 농도에서 메탄올층과 핵산층이 수층과 부탄올층에 비하여 유의적으로(p<0.05) 높게 나타났다. 간암세포인 HepG2의 경우 추출물 $100{\mu}g/mL$의 농도를 기준으로 메탄올층이 유의적으로(p<0.05) 높았고, 핵산층>부탄올층>수층의 순으로 나타났다. 대장암세포인 HT-29 역시 메탄올층이 유의적으로 가장 높았고, 핵산층>수층>부탄올층 순으로 나타났다. 유방암세포인 MCF-7은 메탄올층>핵산층>수층>부탄올층 순으로 효과가 높게 나타났다. 이와 같은 결과로 볼 때 천년초줄기의 항균, 항산화, 항암 효과가 높게 나타나 줄기를 활용한 항균제품이나 여러 가지 가공제품을 개발하는데 기초 자료로 활용할 수 있다.

This study was performed to investigate antimicrobial, antioxidant, and anticancer properties of Opuntia humifusa (OH) stems. OH stems were extracted with hexane, methanol, butanol and water. The methanol and hexane fraction exhibited strong antimicrobial activities on three strains of microbes, Rhodococcus equi, Staphylococcus aureus, and Clostridium perfringens. In the peroxynitrite scavenging effect ($ONOO^-$) of OH stems, the antioxidative activity of methanol, butanol and water fraction but not hexane fraction showed significant increases in a concentration-dependent manner. The DPPH radical scavenging activities of OH stems were high in the butanol fraction compared with other fractions. Anti-proliferation effects on the B16-F10, HepG2, HT29, and MCF-7 cell lines were significantly higher in the methanol and hexane fractions than in the water and butanol fractions at $100{\mu}g/mL$ concentration of extracts. These results suggest that OH stems can be used for the development of functional foods with biological activity.

키워드

참고문헌

  1. Kim TJ. 1996. Korean resources plants. Seoul National University in press, Seoul, Korea. p 140-141.
  2. Lee KS, Kim MG, Lee KY. 2004. Antimicrobial effect of the extracts of cactus Cheonnyuncho (Opuntia humifusa) against food borne pathogens. J Korean Soc Food Sci Nutr 33: 1268-1272. https://doi.org/10.3746/jkfn.2004.33.8.1268
  3. Yoon JA, Hahm SW, Park JE, Son YS. 2009. Total polyphenol and flavonoid of fruit extract of Opuntia humifusa and its inhibitory effect on the growth of MCF-7 human breast cancer cells. J Korean Soc Food Sci Nutr 38: 1679-1684. https://doi.org/10.3746/jkfn.2009.38.12.1679
  4. Lee KS, Lee KY. 2010. Biological activity of phenol compound from a cactus Cheonnyuncho (Opuntia humifusa) in Korea. J Korean Soc Food Sci Nutr 39: 1132-1136. https://doi.org/10.3746/jkfn.2010.39.8.1132
  5. Choi JH. 2010. Structural analysis and immuno-stimulating characteristics of Opuntia humifusa. MS thesis. Kyonggi University, Seoul, Korea. p 4.
  6. Lee KS, Oh CS, Lee KY. 2005. Antioxidative effect of the fractions extracted from a cactus Cheonnyuncho (Opuntia humifusa). J Korean Food Sci Technol 37: 474-478.
  7. Choi HJ, Park SC, Hong TH. 2005. Anti-tumor activity of fermented lipid Oputia humifusa in cervical cancer cells and its chemical composition. J Korean Soc Food Sci Nutr 34: 1525-1530. https://doi.org/10.3746/jkfn.2005.34.10.1525
  8. Park MK, Lee YJ, Kang ES. 2005. Hepato protective effect of Cheonnyuncho (Opuntia humifusa) extract in rats treated carbon tetrachloride. Korean J Food Sci Technol 37: 822-826.
  9. Choi JH, Shin KS. 2011. Structural analysis of anti-metastatic polysaccharide isolated from Opuntia humifusa. J Korean Soc Food Sci Nutr 40: 214-222. https://doi.org/10.3746/jkfn.2011.40.2.214
  10. Davidson PM, Parish ME. 1989. Methods for testing the efficacy of food antimicrobials. Food Technol 1: 148-155.
  11. Crow JP. 1997. Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide 1: 145-157. https://doi.org/10.1006/niox.1996.0113
  12. Chung HJ. 2000. Antioxidative and antimicrobial activities of Opuntia ficus indica var. saboten. Korean J Food Cookery Sci 16: 160-166.
  13. Cha MN. 2010. Physicochemical properties and antioxidant activities of Opuntia humifusa. MS Thesis. Chonbuk National University, Jeonju, Korea. p 12-14.
  14. Lee YJ, Jin YR, Lim WC, Ji SM, Choi SH, Jang SY, Lee SK. 2003. A ginsenoside-RH1, a component of ginseng saponin, activates estrogen receptor in human breast carcinoma MCF-7 cells. J Steroid Biochem Mol Biol 84: 463-468. https://doi.org/10.1016/S0960-0760(03)00067-0
  15. Byun KS, Lee YW, Jin J, Lee MK, Lee HY, Yu CY, Lee JH. 2005. Genotoxicity and cytotoxicity in human cancer and normal cell lines of the extracts of Rhododendron brachycarpum D. Don leaves. Kor J Med Corp Sci 13: 199-205.
  16. Ahn EM, Han JT, Kwon BM, Kim SH, Baek NI. 2008. Anti-cancer activity of flavonoids from Aceriphyllum rossii. J Korean Soc Appl Biol Chem 51: 309-315. https://doi.org/10.3839/jksabc.2008.054

피인용 문헌

  1. Physicochemical properties and antioxidant activity of Korean cactus (Opuntia humifusa) cladodes vol.54, pp.3, 2013, https://doi.org/10.1007/s13580-013-0145-4
  2. Opuntia humifusa (Raf.) Raf. f. jeollaensis E. J. Kim & S. S. Whang, a new forma based on three DNA markers vol.44, pp.3, 2014, https://doi.org/10.11110/kjpt.2014.44.3.181
  3. Chemical composition and antioxidant activity of Korean cactus (Opuntia humifusa) fruit vol.22, pp.2, 2013, https://doi.org/10.1007/s10068-013-0110-0
  4. Quality Characteristics during Storage of Rice Makgeolli Added with Cheonnyuncho Fermentative Extract vol.29, pp.6, 2013, https://doi.org/10.9724/kfcs.2013.29.6.679
  5. Chemical compositions and antioxidant activities of Cheonnyuncho (Opuntia humifusa) stems and fruit vol.23, pp.1, 2016, https://doi.org/10.11002/kjfp.2016.23.1.89
  6. Quality Characteristics of Gat Kimchi Added with Cheonnyuncho Water Extract during Cold Storage vol.45, pp.12, 2016, https://doi.org/10.3746/jkfn.2016.45.12.1808
  7. Optimization of Muffin with Opuntia humifusa Powder using Response Surface Methodology vol.25, pp.4, 2012, https://doi.org/10.9799/ksfan.2012.25.4.911
  8. Functional component analysis and physical property of Cheonnyuncho (Opuntia humifusa) powder vol.22, pp.6, 2015, https://doi.org/10.11002/kjfp.2015.22.6.838
  9. Antiproliferative Effect of Opuntia humifusa Ethanol Extract on Human Carcinoma HT-29 Cells vol.43, pp.12, 2014, https://doi.org/10.3746/jkfn.2014.43.12.1827
  10. 천년초 줄기의 수확 시기 별 외관 및 성분 품질 특성 vol.23, pp.4, 2012, https://doi.org/10.11002/kjfp.2016.23.4.502
  11. 천년초 저온 감압 추출액의 특성 분석 및 화장품 적용 vol.44, pp.4, 2012, https://doi.org/10.15230/scsk.2018.44.4.381
  12. Lactobacillus plantarum으로 발효한 천년초 선인장 열매의 발효특성 및 in vitro 항비만 효과 vol.34, pp.1, 2012, https://doi.org/10.7318/kjfc/2019.34.1.75
  13. Physiological Activities of Opuntia monacantha Haw. Fruit and Stem vol.50, pp.5, 2012, https://doi.org/10.3746/jkfn.2021.50.5.456