Korean Circulation Journal

  • 제42권3호
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  • Pages.154-163
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  • 2012
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  • 1738-5520(pISSN)
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  • 1738-5555(eISSN)
대한심장학회 (The Korean Society of Cardiology)
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A Comparative Study on Mechanical and Biochemical Properties of Bovine Pericardium After Single or Double Crosslinking Treatment

  • Jang, Woo-Sung (Seoul National University Hospital, Clinical Research Institute, Xenotransplantation Research Center) ;
  • Choi, Sun-Young (Seoul National University Hospital, Clinical Research Institute, Xenotransplantation Research Center) ;
  • Kim, Soo-Hwan (Seoul National University Hospital, Clinical Research Institute, Xenotransplantation Research Center) ;
  • Yoon, Eun-Jeung (Seoul National University Hospital, Clinical Research Institute, Xenotransplantation Research Center) ;
  • Lim, Hong-Gook (Seoul National University Hospital, Clinical Research Institute, Xenotransplantation Research Center) ;
  • Kim, Yong-Jin (Seoul National University Hospital, Clinical Research Institute, Xenotransplantation Research Center)
  • 발행 : 2012.03.31
⟨ 이전 논문 다음 논문 ⟩

초록

Background and Objectives: Glutaraldehyde (GA) has been used as a representative method of tissue preservation in cardiovascular surgery. However, GA has showed limited durability including calcification, mechanical failure and toxicity. To overcome those unsolved problems, we analyzed the crosslinking differences of primary amines, GA and genipin in their mechanical and biochemical properties with a single or double crosslinking agent for clinical application. Materials and Methods: Samples were divided into 3 groups; control, single crosslinking fixation and double crosslinking fixation after decellurarization using bovine pericardium. For analysis of the biochemical and mechanical properties of each crosslinking method, tensile strength, percentage strain, thermal stability, resistance to pronase, nynhydrin and cytotoxicity test were studied. Results: Combined hexamethylene diamine and suberic acid in the carbodiimide hydrochloride/N-hydroxysucinimide solution (EDC/NHS) after decellurarization, tensile strength and strain percentage were not statistically significant compared to the single crosslinking treated groups (p>0.05). Tissue crosslinking stability was weak in single treatment of diphenylphosphoryl azide, suberic acid, low concentration of EDC, hexamethylene diamine and procyanidin groups, but thermal stability and resistance to the pronase and ninhydrin were markedly increased in concentrated EDC/NHS or after combined double treatment with low concentration of GA or genipin (p<0.001). Conclusion: Single or double crosslinking with low concentration of carbodiimide, diphenylphosphonyl azide, procyanidin, suberic acid and hexane diamine were not as effective in mechanical, biochemical, cytotoxic and crosslinking properties compared to GA or genipin fixation, but their mechanical and chemical properties were much improved when combined with low concentrations of GA or genipin in the double crosslinking process.

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참고문헌

  1. Lee CR, Grodzinsky AJ, Spector M. The effects of cross-linking of collagen- glycosaminoglycan scaffolds on compressive stiffness, chondrocyte- mediated contraction, proliferation and biosynthesis. Biomaterials 2001;22:3145-54. https://doi.org/10.1016/S0142-9612(01)00067-9
  2. Speer DP, Chvapil M, Eskelton CD, Ulreich J. Biological effects of residual glutaraldehyde-tanned collagen biomaterials. J Biomed Mater Res 1980;14:753-64. https://doi.org/10.1002/jbm.820140607
  3. Petite H, Rault I, Huc A, Menasche P, Herbage D. Use of the acyl azide method for cross-linking collagen-rich tissues such as pericardium. J Biomed Mater Res 1990;24:179-87. https://doi.org/10.1002/jbm.820240205
  4. Anselme K, Petite H, Herbage D. Inhibition of calcification in vivo by acyl azide cross-linking of a collagen-glycosaminoglycan sponge. Matrix 1992;12:264-73. https://doi.org/10.1016/S0934-8832(11)80078-8
  5. Petite H, Duval JL, Frei V, Abdul-Malak N, Sigot-Luizard MF, Herbage D. Cytocompatibility of calf pericardium treated by glutaraldehyde and by the acyl azide methods in an organotypic culture model. Biomaterials 1995;16:1003-8. https://doi.org/10.1016/0142-9612(95)94908-4
  6. Olde Damink LH, Diijkstra PJ, van Luyn MJ, van Wachem PB, Nieuwenhuis P, Feijen J. Cross-linking of dermal sheep collagen using a watersoluble carbodiimide. Biomaterials 1996;17:765-73. https://doi.org/10.1016/0142-9612(96)81413-X
  7. Olde Damink LH, Dijkstra PJ, van Luyn MJ, van Wachem PB, Nieuwenhuis P, Feijen J. In vitro degradation of dermal sheep collagen cross-linked using a water-soluble carbodiimide. Biomaterials 1996;17:679-84. https://doi.org/10.1016/0142-9612(96)86737-8
  8. Olde Damink LH, Dijkstra PJ, van Luyn MJ, van Wachem PB, Nieuwenhuis P, Feijen J. Crosslinking of dermal sheep collagen using hexamethylene diisocyanate. J Mater Sci Mater Med 1995;6:429-34. https://doi.org/10.1007/BF00120286
  9. Hanthamrongwit M, Reid WH, Grant MH. Chondroitin-6-sulphate incorporated into collagen gels for the growth of human keratinocytes: the effect of cross-linking agents and diamines. Biomaterials 1996;17: 775-80. https://doi.org/10.1016/0142-9612(96)81414-1
  10. Shen SH, Sung HW, Tu R, et al. Characterization of a polyepoxy compound fixed porcine heart valve bioprosthesis. J Appl Biomater 1994; 5:159-62. https://doi.org/10.1002/jab.770050209
  11. Xi T, Ma J, Tian W, Lei X, Long S, Xi B. Prevention of tissue calcification on bioprosthetic heart valve by using epoxy compounds: a study of calcification test in vitro and in vivo. J Biomed Mater Res 1992;26: 1241-51. https://doi.org/10.1002/jbm.820260913
  12. Nimni ME, Cheung DT, Strates B, Kodama M, Sheikh K. Bioprosthesis derived from cross-linked and chemically modified collagenous tissues. In: Nimni ME, editor. Collagen, Vol. III. Boca Raton, FL: CRC Press; 1988. p.1-38.
  13. Zeeman R, Dijkstra PJ, van Wachem PB, et al. Successive epoxy and carbodiimide cross-linking of dermal sheep collagen. Biomaterials 1999;20:921-31. https://doi.org/10.1016/S0142-9612(98)00242-7
  14. Sung HW, Hsu CS, Wang SP, Hsu HL. Degradation potential of biological tissues fixed with various fixatives: an in vitro study. J Biomed Mater Res 1997;35:147-55. https://doi.org/10.1002/(SICI)1097-4636(199705)35:2<147::AID-JBM2>3.0.CO;2-N
  15. Timkovich R. Detection of the stable addition of carbodiimide to proteins. Anal Biochem 1977;79:135-43. https://doi.org/10.1016/0003-2697(77)90387-6
  16. Jang WS, Kim YJ, Kim SH. Effects on tensile strength and elasticity after treatment with glutaraldehyde, solvent, decellularization and detoxification in fresh bovine pericardium. Korean J Thorac Cardiovasc Surg 2010;43:1-10. https://doi.org/10.5090/kjtcs.2010.43.1.1
  17. Sung SC, Kim YJ, Choi SY, Park JE, Kim KW, Kim WH. A study on an effective decelularization technique for a xenograft cardiac valve: the effect of osmotic treatment with hypotonic solution. Korean J Thorac Cardiovasc Surg 2008;41:679-86.
  18. Cho S, Kim YJ, Kim SH, Choi SH. Biaxial strain analysis of various fixation models in porcine aortic and pulmonary valves. Korean J Thorac Cardiovasc Surg 2009;42:566-75.
  19. Cho S, Kim YJ, Kim SH, Park JE, Kim WH. Comparison of the uniaxial tensile strength, elasticity and thermal stability between glutaraldehyde and glutaraldehyde with solvent fixation in xenograft cardiovascular tissue. Korean J Thorac Cardiovasc Surg 2009;42:165.
  20. Hansen DB, Joullie MM. The development of novel ninhydrin analogues. Chem Soc Rev 2005;34:408-17. https://doi.org/10.1039/b315496n
  21. Jayakrishnan A, Jameela SR. Glutaraldehyde as a fixative in bioprostheses and drug delivery matrices. Biomaterials 1996;17:471-84. https://doi.org/10.1016/0142-9612(96)82721-9
  22. Gratzer PF, Lee JM. Control of pH alters the type of cross-linking produced by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) treatment of acellular matrix vascular grafts. J Biomed Mater Res 2001; 58:172-9. https://doi.org/10.1002/1097-4636(2001)58:2<172::AID-JBM1004>3.0.CO;2-9
  23. Moore MA, Chen WM, Phillips RE, Bohachevsky IK, McIlroy BK. Shrinkage temperature versus protein extraction as a measure of stabilization of photooxidized tissue. J Biomed Mater Res 1996;32:209-14. https://doi.org/10.1002/(SICI)1097-4636(199610)32:2<209::AID-JBM9>3.0.CO;2-X
  24. Zheng LT, Ryu GM, Kwon BM, Lee WH, Suk K. Anti-inflammatory effects of catechols in lipopolysaccharide-stimulated microglia cells: inhibition of microglial neurotoxicity. Eur J Pharmacol 2008;588:106-13. https://doi.org/10.1016/j.ejphar.2008.04.035
  25. Dongmo AB, Kamanyi A, Anchang MS, et al. Anti-inflammatory and analgesic properties of the stem bark extracts of Erythrophleum suaveolens (Caesalpiniaceae), Guillemin & Perrottet. J Ethnopharmacol 2001;77:137-41. https://doi.org/10.1016/S0378-8741(01)00296-3
  26. Blazso G, Gabor M, Rohdewald P. Antiinflammatory activities of procyanidin- containing extracts from Pinus pinaster Ait: after oral and cutaneous application. Pharmazie 1997;52:380-2.
  27. Packer L, Rimbach G, Virgili F. Antioxidant activity and biologic properties of a procyanidin-rich extract from pine (Pinus maritima) bark, pycnogenol. Free Radic Biol Med 1999;27:704-24. https://doi.org/10.1016/S0891-5849(99)00090-8
  28. Bretti C, Crea F, Foti C, Smmartano S. Solubility and activity coefficients of acidic and basic nonelectolytes in aqueous salt solutions. J Chem Eng Data 2005;50:1761-7. https://doi.org/10.1021/je0502039
  29. Schmidt CE, Baier JM. Acellular vascular tissue: natural biomaterials for tissue repair and tissue engineering. Biomaterials 2000;21:2215-31. https://doi.org/10.1016/S0142-9612(00)00148-4

피인용 문헌

  1. Biomaterial characterization of off‐the‐shelf decellularized porcine pericardial tissue for use in prosthetic valvular applications vol.12, pp.7, 2012, https://doi.org/10.1002/term.2686
  2. Impact of Clinically Relevant Elliptical Deformations on the Damage Patterns of Sagging and Stretched Leaflets in a Bioprosthetic Heart Valve vol.9, pp.3, 2012, https://doi.org/10.1007/s13239-018-0366-x
  3. Epoxy Chitosan-Crosslinked Acellular Bovine Pericardium with Improved Anti-calcification and Biological Properties vol.3, pp.4, 2020, https://doi.org/10.1021/acsabm.0c00067