Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Effects of Dietary Supplementation with Nucleotide on Growth Performance, Feed Utilization, and Non-Specific Immune Responses of Nile Tilapia Oreochromis niloticus

사료 내 nucleotide 첨가가 틸라피아(Oreochromis niloticus)의 성장, 사료효율 및 비특이적 면역력에 미치는 영향

  • Song, Jin-Woo (Department of Marine Life Sciences, Jeju National University) ;
  • Lim, Se-Jin (Department of Global Technical Marketing, CJ CheilJedang Corporation) ;
  • Oh, Dae-Han (Department of Marine Life Sciences, Jeju National University) ;
  • Cha, Ji-Hoon (Department of Marine Life Sciences, Jeju National University) ;
  • Lee, Kyeong-Jun (Marine and Environment Research Institute, Jeju National University)
  • 송진우 (제주대학교 해양생명과학과) ;
  • 임세진 (CJ 제일제당주식회사 Global 기술마케팅) ;
  • 오대한 (제주대학교 해양생명과학과) ;
  • 차지훈 (제주대학교 해양생명과학과) ;
  • 이경준 (제주대학교 해양과환경연구소)
  • Received : 2012.07.31
  • Accepted : 2012.11.29
  • Published : 2012.12.31
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Abstract

The present study examined the effects of dietary supplementation with nucleotide (inosine monophosphate product, IMP) on the growth performance, feed utilization, and non-specific immune responses of juvenile tilapia Oreochromis niloticus. Triplicate groups of tilapia (initial body weight, $7.4{\pm}0.04$ g) were fed experimental diets containing 0%, 0.05%, 0.1%, and 0.2% IMP. Fish were fed six times a day until apparent satiation for 13 weeks. At the end of the feeding trial, final body weight and food utilization of fish fed 0.1% IMP were significantly higher than those of fish fed the control diet. Results of hematological parameters were not affected by dietary IMP. However, blood protein level was significantly higher in the 0.05% treatment, as compared to that of the control and 0.2% IMP diets. Myeloperoxidase activity was higher in fish fed 0.1% IMP than in fish fed the control and 0.2% IMP diets. These results suggest that dietary supplementation with IMP can enhance the growth performance, feed utilization, and innate immune response of juvenile tilapia. The optimal IMP supplementation level appears to be 0.1% in practical feed formulations for tilapia.

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References

  1. Adamek Z, Hamackova J, Kouril J, Vachta R and Stibranyiova I. 1996. Effect of Ascogen probiotics supplementation on farming success in rainbow trout (Oncorhynchus mykiss ) and wels (Silurus glais ) under conditions of intensive culture. Krmiva (Zagreb) 38, 11-20.
  2. AOAC. 1995. Official methods of analysis. 16th ed. Association of Official Analytical Chemists, Arlington, Virginia, U.S.A, 1298.
  3. Carver J, Cox W and Barness L. 1990. Dietary nucleotide effects upon murine natural killer cell activity and macrophage activation. J Parenter Enteral Nutr 14, 18-22. https://doi.org/10.1177/014860719001400118
  4. Carver JD and Walker WA. 1995. The role of nucleotides in human nutrition. J Nutr Biochem 6, 58-72. https://doi.org/10.1016/0955-2863(94)00019-I
  5. Cheng Z, Buentello A and Gatlin III DM. 2011. Dietary nucleotides influence immune responses and intestinal morphology of red drum Sciaenops ocellatus . Fish Shellfish Immunol 30, 143-147. https://doi.org/10.1016/j.fsi.2010.09.019
  6. Cosgrove M. 1998. Nucleotides. Nutrition 14, 748-751. https://doi.org/10.1016/S0899-9007(98)00075-6
  7. El-Sayed AFM. 2006. Tilapia Culture. CABI Publishing, CABI International, Wallingford, Oxfordshire, UK.
  8. Folch J, Lee M and Sloane-Stanley GH. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226, 497-509.
  9. Gil A. 2002. Modulation of the immune responses mediated by dietary nucleotides. Eur Clin Nutr 56, 1-4. https://doi.org/10.1038/sj.ejcn.1601283
  10. Grimble GK and Westwood OMR. 2000. Nucleotides. In: German JB, Keen CL. (Eds.), Nutrition and Immunology: Principles and Practice. Humana Press Inc., Totowa, NJ, USA, 135-144.
  11. Hardy RW and Tacon AGJ. 2002. Fish meal: Historical uses, production trends, and future outlook for sustainable supplies. In: Stickney RR, MaVey JM. (Eds.), Responsible Marine Aquaculture. CABI Publishing, Wallingford, UK, 311-325.
  12. Jha AK, Pal AK, Sahu NP, Kumar S and Mukherjee SC. 2007. Haemato-immunological responses to dietary yeast RNA, $\omega$-3 fatty acid and $\beta$-carotene in Catla catla juveniles. Fish Shellfish Immunol 23, 917-927. https://doi.org/10.1016/j.fsi.2007.01.011
  13. Kim SS and Lee KJ. 2008. Effects of dietary kelp (Ecklonia cava ) on growth and innate immunity in juvenile olive flounder Paralichthys olivaceus . Aquac Res 39, 1687-1690.
  14. Kim SS, Galza GB, Pham MA, Jang JW, Oh DH, Yeo IK and Lee KJ. 2009. Effects of dietary supplementation of a Meju, fermented soybean meal, and Aspergillus oryzae for juvenile parrot fish (Oplegnathus fasciatus ). Asian Aust J Anim Sci 22, 849-856. https://doi.org/10.5713/ajas.2009.80648
  15. Kim SS, Song JW, Lim SJ, Jeong JB, Jeon YJ, Yeo IK and Lee KJ. 2010. Effects of dietary supplementation of fermented garlic powder on immune responses, blood components, and disease resistance against principal fish disease of juvenile olive flounder, Paralichthys olivaceus in low temperature season. J Anim Sci & Technol 52, 337-346. https://doi.org/10.5187/JAST.2010.52.4.337
  16. Kiyohara S, Hidaka I and Tamura T. 1975. Gustatory response in the puffer-II. Single fiber analysis. Bull Jpn Soc Sci Fish 41, 383-391. https://doi.org/10.2331/suisan.41.383
  17. Kubitza F, Lovshin LL and Lovell RT. 1997. Identification of feed enhancers for juvenile largemouth bass, Micropterus salmoides . Aquaculture 148, 191-200. https://doi.org/10.1016/S0044-8486(96)01417-2
  18. Kumar S, Sahu NP, Pal AK, Choudhury D, Yengkokpam S and Mukherjec SC. 2005. Effect of dietary carbohydrate on haematology, respiratory burst activity and histological changes in L. rohita juveniles. Fish Shellfish Immunol 19, 331-344. https://doi.org/10.1016/j.fsi.2005.03.001
  19. Kumari J and Sahoo PK. 2005. Effects of cyclophosphamide on the immune system and disease resistance of Asian catfish Clarias batrachus . Fish Shellfish Immunol 19, 307-316. https://doi.org/10.1016/j.fsi.2005.01.008
  20. Lee SM and Lim TJ. 2000. Effects of herb as an additive in formulated diet on growth and body composition of larval ayu (Plecoglossus altivelis ). J East Coastal Res 11, 35-42.
  21. Li P and Gatlin III DM. 2006. Nucleotide nutrition in fish: Current knowledge and future applications. Aquaculture 251, 141-152. https://doi.org/10.1016/j.aquaculture.2005.01.009
  22. Li P, Gatlin III DM and Neill WH. 2007. Dietary supplementation of a purified nucleotide mixture transiently enhanced growth and feed utilization of juvenile red drum, Sciaenops ocellatus . J World Aquacult Soc 38, 281-286. https://doi.org/10.1111/j.1749-7345.2007.00096.x
  23. Lin YH, Wang H and Shiau SY. 2009. Dietary nucleotide supplementation enhances growth and immune responses of grouper, Epinephelus malabaricus . Aquacult Nutr 15, 117-122. https://doi.org/10.1111/j.1365-2095.2007.00561.x
  24. Low C, Wadsworth S, Burrells C and Secombes CJ. 2003. Expression of immune genes in turbot (Scophthalmus maximus ) fed a nucleotide-supplemented diet. Aquaculture 221, 23-40. https://doi.org/10.1016/S0044-8486(03)00022-X
  25. Mackie AM. 1973. The chemical basis of food detection in the lobster Homarus gammarus . Mar Biol 21, 103-108. https://doi.org/10.1007/BF00354605
  26. Mateo CD, Stein HH. 2004. Nucleotide deficiencies in starter diets for weanling pigs. Nutritional biotechnology in the feed industry. In: Proceedings of Alltech's 20th annual symposium, Lexington, KY, U.S.A, 1-55.
  27. Rudolph FB. 1994. The biochemistry and physiology of nucleotides. J Nutr 124, 124-127.
  28. Sakai M, Taniguchi K, Mamoto K, Ogawa H and Tabata M. 2001. Immunostimulant effects of nucleotide isolated from yeast RNA on carp, Cyprinus carpio L. J Fish Dis 24, 433-438. https://doi.org/10.1046/j.1365-2761.2001.00314.x
  29. Sankaran K and Shanto G. 1972. On the variation in catalytic activity of lysozyme in fishes. Indian J Biochem Biophys 91, 162-165.
  30. Shiau SY and Yu YP. 1999. Dietary supplementation of chitin and chitosan depresses growth in tilapia, Oreoxhromis niloticus${\times}$O. aureus. Aquaculture 179, 439-446. https://doi.org/10.1016/S0044-8486(99)00177-5
  31. Song JW, Jang JW, Kim SS, Oh DH, Cha JH and Lee KJ. 2011. Effect of dietary supplementation with alga (Hizikia fusiformis and Ecklonia cava ) on the non-specific immune responses of parrot fish Oplegnathus fasciatus. Kor J Fish Aquat Sci 44, 332-338. https://doi.org/10.5657/KFAS.2011.0332
  32. Tacon AGF and Metian M. 2009. Fishing for aquaculture: nonfood use of small pelagic forage fish-a global perspective. Rev Fish Sci 17, 305-317. https://doi.org/10.1080/10641260802677074
  33. Tahmasebi-Kohyani A, Keyvanshokooh S, Nematollahi A, Mahmoudi N and Pasha-Zanoosi H. 2011. Dietary administration of nucleotides to enhance growth, humoral immune responses, and disease resistance of the rainbow trout (Oncorhynchus mykiss ) fingerlings. Fish Shellfish Immunol 30,189-193. https://doi.org/10.1016/j.fsi.2010.10.005
  34. Welker TL, Lim C, Yildirim-Aksoy M and Klesius PH. 2011. Effects of dietary supplementation of a purified nucleotide mixture on immune function and disease and stress resistance in channel catfish, Ictalurus punctatus. Aquac Res 42, 1878-1889. https://doi.org/10.1111/j.1365-2109.2010.02794.x

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