Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Anti-oxidative and anti-inflammatory effects of Odukhwan and Sasinhwan in RAW264.7 cells

오덕환(五德丸)과 사신환(四神丸)의 항산화 및 항염증 효과

  • Kim, Tae-Hoon (Dept. of Prescriptionology. College of Korean Medicine, Dong-Guk University) ;
  • Cho, Hyun-Jin (Dept. of Prescriptionology. College of Korean Medicine, Dong-Guk University) ;
  • Park, Sun-Dong (Dept. of Prescriptionology. College of Korean Medicine, Dong-Guk University)
  • 김태훈 (동국대학교 한의과대학 방제학교실) ;
  • 조현진 (동국대학교 한의과대학 방제학교실) ;
  • 박선동 (동국대학교 한의과대학 방제학교실)
  • Received : 2012.11.17
  • Accepted : 2012.12.16
  • Published : 2012.12.30
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Abstract

Objectives : The aim of this study was verification of the anti-oxidative and anti-inflammatory effects of Odukhwan(ODH) and Sasinhwan(SSH) in mouse macrophage, RAW264.7 cells. Methods : To investigate the anti-oxidative effect and scavenging activities of DPPH radical, superoxide anions, nitric oxide and peroxynitrite were measured. Cytotoxic activity of extract of ODH and SSH on RAW264.7 cells was measured using MTS assay. To proof the reductive activity of intracellular oxidation, DCFH-DA assay was performed. The nitric oxide(NO) production was measured and pro-inflammatory cytokines and $PGE_2$ were measured by ELISA kit. The levels of inducible nitric oxide synthase(iNOS), cyclooxygenase-2(COX-2) and nuclear NF-${\kappa}B$ p65 expression were detected by western blot. Results : After those analyses, we bring to a conclusion as follows. Both herbal formulations scavenged DPPH radical and nitric oxide. But ODH had no scavenging activity of superoxide anions and SSH had low scavenging activity in peroxynitrite. And the results indicated that ODH and SSH inhibited the LPS-induced NO, $PGE_2$ production and iNOS, COX-2 expression accompanied by an attenuation of IL-$1{\beta}$ and IL-6 production in RAW264.7 cells. They also have suppression effects of LPS-induced NF-${\kappa}B$ activation. Conclusions : ODH and SSH have anti-oxidative and anti-inflammatory effects and they may be a part of database for development of new anti-oxidative and anti-inflammatory drugs.

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References

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