Journal of Sericultural and Entomological Science (한국잠사곤충학회지)

Korean Society of Sericultural Science (한국잠사학회)
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Seasonal Resveratrol Contents of Wild-type Mulberry Leaves Collected from Gangwon Province in Korea

강원도에서 채취한 야생 뽕잎의 생육 시기별 resveratrol 함량

  • Published : 2012.06.30
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Abstract

Resveratrol is naturally occurring phytoalexin compounds produced by grape berries, peanuts, pine tree, mulberry fruits and their products in response to stress such as fungal infection, heavy metal ions or UV irradiation. The objective of this study was to determine resveratrol contents in wild-type mulberry leaves (Morus alba L., M. bombycis Koidz, and M. Lhou (Ser.) Koidz) collected from Won-ju, In-je and Yang-yang regions, Gangwon province, Korea. Resveratrol contents in wild-type mulberry leaves were higher in Autumn's than in Spring's, and higher than 16 cultivar's. Among the samples, 'No. 3', collected from Won-ju region and 'No. 7' collected from In-je showed high resveratrol contents of 539 and $491{\mu}g$/100 g DW (dry weight; DW), respectively, through the collection time. Therefore, two mulberry trees might be promising as potent resources for mulberry breeding and functional material development.

야생 뽕나무의 자원화 및 이용기술을 개발하기 위해 강원도 원주, 인제, 양양 3개 지역에서 생육 단계별로 채취한 야생 뽕나무의 뽕잎을 대상으로 레스베라트롤 함량을 분석함으로써 뽕 품종의 육종 효율을 높이는 동시에 기능성 및 이용 효율이 기대되는 뽕 유전자원을 선발하고자 하였다. 야생 뽕잎의 레스베라트롤 함량은 오디에 비해 평균 함량이 상당히 낮았으나 장려뽕에 비해서는 높은 경향을 나타냈다. 생육시기별 야생 뽕잎의 평균 레스베라트롤 함량은 추기의 뽕잎이 춘기보다 함량이 높았다. 즉 춘기 5개엽기 어린잎의 평균 레스베라트롤 함량은 $344{\mu}g$/100 g이었으며 뽕잎의 생육시기가 경과함에 따라 $356{\mu}g$/100 g DW, $273{\mu}g$/100 g DW, $253{\mu}g$/100 g DW 이었다. 추기 뽕잎의 레스베라트롤 함량은 시기가 경과함에 따라 $438{\mu}g$/100 g DW, $476{\mu}g$/100 g DW, $598{\mu}g$/100 g DW로 증가하였다. 따라서 레스베라트롤의 원료로서 뽕잎을 이용하고자 하는 경우 봄뽕보다는 가을뽕을 이용하는 것이 바람직하며, 수세 후 바로 실온에 음건하여 사용하는 방법이 레스베라트롤의 함량이 높을 것으로 판단되었다. 원주 지역의 No. 3 개체의 야생 뽕잎은 생육시기 모두 평균 함량보다 높았으며 전체 평균 $539{\mu}g$/100 g DW으로서 10개체의 뽕나무 중 가장 높은 함량을 나타냈다. 다음으로 인제 지역의 No. 7 개체의 뽕잎은 전체 평균 $491{\mu}g$/100 g DW으로 높았다. 따라서 두 개체의 뽕나무는 레스베라트롤 함량이 높은 육종소재 및 이용 가능성을 검토할 만한 자원으로 선발하였다.

Keywords

References

  1. Adrian M, Jeandet P, Bessis R, Joubert JM (1996) Induction of phytoalexin (resveratrol) synthesis in grapevine leaves treated with aluminum chloride $(AlCl_{3})$. J Agric Food Chem 44, 1979-1981. https://doi.org/10.1021/jf950807o
  2. Arora MK, Strange RN (1991) Phytoalexin accumulation in groundnuts in response to wounding. Plant Sci 78, 157-163. https://doi.org/10.1016/0168-9452(91)90194-D
  3. Creasy LL, Coffee M (1988) Phytoalexin production potential of grape berries. J Am Soc Hortic Sci 113, 230-234.
  4. Dixon RA (2001) Natural products and plant disease resistance. Nature 411, 843-847. https://doi.org/10.1038/35081178
  5. Fontecave M, Lepoivre M, Elleingand E, Gerez C, Guittet O (1998) Resveratrol, a remarkable inhibitor of ribonucleotide reductase. FEBS Lett 421(3), 277-279. https://doi.org/10.1016/S0014-5793(97)01572-X
  6. Fremont L (2000) Biological effects of resveratrol. Life Sci 66, 663-673. https://doi.org/10.1016/S0024-3205(99)00410-5
  7. Fremont L, Belguendou L, Delpal S (1999) Antioxidant activity of resveratrol and alcohol-free wine polyphenols realted to LDL oxidation and polyunsaturated fatty acids. Life Sci 64, 2511-2521. https://doi.org/10.1016/S0024-3205(99)00209-X
  8. Ingham JL (1976) 3,5,4'-Trihydroxystilbene as a phytoalexin from groundnuts (Arachis hypogaea). Phytochemistry 15, 1791-1793. https://doi.org/10.1016/S0031-9422(00)97494-6
  9. Jeandel P, Bessis R, Gautheron B (1991) The production of resveratrol (3,5,4'-thrhydroxystillbene) by grape berries in different developmental stages. Am J Enol Vitic 41, 41-46.
  10. Keen NT (1975) The isolation of phytoalexins from germinating seeds of Cicer arictinum, Vigna sinensis, Arachis hypogaea and other plants. Phytopathology 65, 91-92. https://doi.org/10.1094/Phyto-65-91
  11. Kim DJ, Kim SG, Kim MH, Lee HB, Lee JS (2003) Analysis od trans-Resveratrol Contents of Grape and Grape Products Consumed in Korea. Korean J Food Sci Technol 35(5), 764-768.
  12. Kim HB, Kim SL (2003) Identification of C3G(cyanidin-3-glucoside) from Mulberry Fruits and Quantification with Different Varieties. Korean J Seric Sci 45(2), 90-95.
  13. Kim HB, Kim SL (2004) Quantification and Varietal Variation of Rutin in Mulberry Fruits. Korean J Seric Sci 46(1), 1-5.
  14. Kim HB, Kim JB, Kim SL (2005) Varietal Analysis and Quantification of Resveratrol in Mulberry Fruits. Korean J Seric Sci 47(2), 51-55.
  15. Kim HB, Kim JB, Kim SL, Koh SH, Seok YS, Kim YS, Sung GB, Kang PD (2011) Quantitative Analysis of Resveratrol in Mulberry Leaves. Korean J Crop Sci 56(1), 23-28. https://doi.org/10.7740/kjcs.2011.56.1.023
  16. Kim HB, Kim SL, Kang SW (2004) Varietal Analysis and Quantification of Amino Acid in Mulberry Fruits. Korean J Seric Sci 46(2), 47-53.
  17. Kim HB, Kim SL, Sung GB, Nam HW, Chang SJ, Moon JY (2003) Quantification and Varietal Variation of Fatty Acids in Mulberry Fruits. Korean J Seric Sci 45(2), 75-79.
  18. Lee HS, Sur EY, Kim WK (2004) Resveratrol Induces Apoptosis in SW480 Human Colon Cancer Cell Lines. Food Sci Biotechnol 13(1), 80-84.
  19. MacCarrone M, Lorenzon T, Guerrieri P, Agro AF (1999) Resveratrol prevents apoptosis in K562 cells by inhibiting lipoxygenase and cyclooxygenase activity. Eur J Biochem 265, 37-34.
  20. Pace-Asciak CR, Hahn SE, Diamandis EP, Soleas G, Goldberg DM (1995) The red wine phenolics trans-resveratrol and quercetin block human platelet aggregation and eicosanoid synthesis: implications for protection against coronary heart disease. Clin Chim Acta 235, 207-219. https://doi.org/10.1016/0009-8981(95)06045-1
  21. Ragione FD, Cucciolla V, Borriello A, Pietra VD, Racioppi L, Soldati G, Manna C, Galletti P, Zappia V (1998) Resveratrol arrests the cell division cycle at S/G2 phase transition. Biochem Biophys Res Commun 250(1), 53-58. https://doi.org/10.1006/bbrc.1998.9263
  22. Sanders TH, McMichael RW (1997) Occurrence of resveratrol in edible peanuts. Book of Abstract, Las Vegas, NV; American Chemical Society: Washington, DC. Abstr. AGFD 214, 33.
  23. Sanders TH, McMichael RW, Hendrix KW (2000) Occurrence of Resveratrol in Edible Peanuts. J Agric Food Chem 48, 1243-1246. https://doi.org/10.1021/jf990737b
  24. Sobolev VS, Cole RJ (1999) trans-Resveratrol content in commercial peanuts and peanut products. J Agric Food Chem 47, 1435-1439. https://doi.org/10.1021/jf9809885
  25. Sobolev VS, Cole RJ, Dorner JW, Yagen B (1995) Isolation, purification, and liquid chromatographic determination of stilbene phytoalexins in peanuts. J Assoc Off Anal Chem Int 78, 1177-1182.
  26. Subbaramaiah K, Michaluart P, Chung WJ, Tanabe T, Telang N, Dannenberg AJ (1999) Resveratrol inhibits cyclooxygenase-2 transcription in human mammary epithelial cell. Ann N Y Acad Sci 889, 214-223. https://doi.org/10.1111/j.1749-6632.1999.tb08737.x
  27. 성규병, 김현복, 강필돈, 김현숙, 김종선, 구희연, 서상덕, 이진근. (2011) 새로 육성한 오디 생산용 뽕나무 품종 "상베리"의 특성. 제54회 (사)한국잠사학회 정기 총회 및 잠사.산업곤충분야 춘계 학술대회. 54(1), 44
  28. 성규병, 김현복, 강필돈 이광길. (2010) 오디 생산용으로 육성한 뽕나무 품종들의 종류 및 특성. 제53회 한국잠사학회 추계 학술 연구발표회. 53(2), 41.
  29. 성규병, 남학우, 이상욱, 김현복, 홍인표, 남성희, 손봉희, 정인모. (2005) 새로 육성한 뽕잎 생산용 "신성뽕"의 특성. 제48회 한국잠사학회 추계 학술연구 발표회. 48(2). 35.

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