Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Lack of Mutation in p53 and H-ras Genes in Phenytoin Induced Gingival Overgrowth Suggests its Non Cancerous Nature

  • Jayaraman, Bhaskar (Department of Periodontia, Sree Balaji Dental College and Hospital, Bharath University) ;
  • Valiathan, Gopalakrishnan Mohan (Department of Periodontia, Sree Balaji Dental College and Hospital, Bharath University) ;
  • Jayakumar, Keerthivasan (Human Genetics Laboratory, Sree Balaji Dental College and Hospital, Bharath University) ;
  • Palaniyandi, Arunmozhi (Department of Periodontia, Rajah Muthiah Dental College and Hospital, Annamalai University) ;
  • Thenumgal, Siji Jacob (Department of Periodontia, Rajah Muthiah Dental College and Hospital, Annamalai University) ;
  • Ramanathan, Arvind (Human Genetics Laboratory, Sree Balaji Dental College and Hospital, Bharath University)
  • Published : 2012.11.30
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Abstract

Background: There have been case reports of oral squamous cell carcinoma arising from gingival overgrowth induced by phenytoin - an antiepileptic drug. However, a detailed analysis for the presence of mutations in p53 and ras genes, which are the two most frequently mutated genes in cancers, in phenytoin induced gingival overgrowth tissues has hitherto not been performed. Methods: Cellular DNA isolated from twenty gingival overgrowth tissues collected from patients undergoing phenytoin therapy were amplified using primers for p53 (exons 5-8) and H-ras (exons 1-2) genes. The PCR amplicons were then gel purified and subjected to direct sequencing analysis to screen for mutations. Results: Direct sequencing of twenty samples of phenytoin induced gingival growth did not identify mutations in any of the exons of p53 and H-ras genes that were analyzed. Conclusion: Our result indicates that mutational alteration of p53 and H-ras genes is infrequent in phenytoin induced gingival growth, which thus suggests a non malignant nature of this pathology. The findings in the present study are clinically significant as a large number of epileptic patients are treated with phenytoin.

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References

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