한국패류학회지 (The Korean Journal of Malacology)

한국패류학회 (The Malacological Society of Korea)
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소라, Batillus cornutus (Lightfoot, 1786) 혈구의 종류 및 미세구조적 특징

Ultrastructural Characteristics and Categorization of the Hemocytes in Spiny Top shell, Batillus cornutus (Lightfoot, 1786) (Gastropoda: Turbinidae)

  • 박정준 (국립수산과학원 양식관리과) ;
  • 김석렬 (국립수산과학원 병리연구과) ;
  • 이정식 (전남대학교 수산생명의학과) ;
  • 박명애 (국립수산과학원 양식관리과) ;
  • 김재원 (강원도립대학교 해양생명과학과) ;
  • 정귀권 (영광정보산업고등학교)
  • Park, Jung Jun (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Kim, Seok-Ryel (Pathology Division, National Fisheries Research and Development Institute) ;
  • Lee, Jung Sick (Department of Aqualife Medicine, Chonnam National University) ;
  • Park, Myoung Ae (Aquaculture Management Division, National Fisheries Research and Development Institute) ;
  • Kim, Jae Won (Department of Marine Life-Science, Gangwon Provincial College) ;
  • Kwon, Jung Gui (Yeonggwang Information Industry High School)
  • 투고 : 2012.11.14
  • 심사 : 2012.12.03
  • 발행 : 2012.12.31
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초록

전라남도 완도군 인근연안에서 채집된 소라, Batillus cornutus의 혈구를 광학현미경과 전자현미경을 이용하여 혈구의 종류 및 미세구조적인 특징을 연구하였다. 광학현미경 상에서 소라의 혈구들은 호염기성 세포로 관찰되었다. 혈구의 형태학적인 특징으로 분류를 하였을 때 소라의 혈구는 모두 8종류로 나눌 수 있었다. 미성숙 혈구는 직경 약 4.5-5.5 ${\mu}m$로서 세포질 내에 지름 2-3 ${\mu}m$인 원형의 핵이 대부분을 차지하고 있었다. 과립세포는 직경 약 7 ${\mu}m$로서 세포질에는 전자밀도가 낮고 크기가 다양한 과립들과 미토콘드리아, 글리코겐 과립들이 관찰되었다. 초자세포는 핵의 크기 및 세포질에 존재하는 미세소관의 특징에 따라 6종류로 세분화 할 수 있었다. 초자세포 III은 라이소좀 및 글리코겐 과립의 형성이 미약하여 식세포의 기능 보다는 영양분의 흡수 및 운반 기능을 수행하는 것으로 판단되며, 특히 초자세포 VI은 형태가 불규칙한 아메바형으로 세포질에 글리코겐 과립의 분포가 높고, 이식포와 자식포가 분포하고 있었다. 따라서 이들 혈구들의 정확한 기능을 파악하기 위해서는 효소학적 및 면역학적 연구를 추가적으로 수행해야 할 것으로 판단된다.

Light and transmission electron microscopy of Batillus cornutus hemocytes revealed differences that the morphological distinctions between blast-like cell, granulocytes and hyalinocytes. Base on the morphological characteristics of the cells, we identified the eight types of hemocytes and present a categorization of the hyalinocytes into six sub-categories. The hemocytes of B. cornutus were observed basophilic cell under the light microscopy. Blast-like cells had a spherical profile with a central nucleus filling almost the whole cell. Granulocytes were characterized by presenting variable numbers of granules. This cell had spherical shape with diameter 7 ${\mu}m$ and smooth endoplasmic reticula, granules, mitochondria, glycogen granules in the cytoplasm. Hyalinocytes were the most abundant cell type. Especially, hyalinocyte VI had iirregular an amoebal shape and observed autophagosome and heterophagosome in the cytoplasm. From these results, it is concluded that there are eight types of cells in the hemolymph of B. cornutus. Further studies are now needed to identify the role of these hemocytes in the enzymological and immunological response.

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