Mass Production of Artificial Seedlings in Hard Clam Meretrix petechialis (Lamarck)

말백합 Meretrix petechialis (Lamarck) 인공종묘의 대량생산

Mass production method on artificial seedling production of hard clam Meretrix petechialis was developed indoor culture system. Spawning of adult clam (SL $65.8{\pm}8.4mm$) was induced using the combined method of air exposure and water temperature raising. The fertilized eggs were developed to D-shaped larvae after 17.7 hours at $27^{\circ}C$ and hatching rate was 6.1%. Shell length (SL) of D-shaped larvae was measured to be $131.4{\pm}2.6{\mu}m$ and thereafter the larvae grew to the settled spats with SL $190.2{\pm}7.5{\mu}m$ in 4 days. Estimated survival rate of settled spats was 48.1%. Spat collection on 130,000 spats with SL $0.19{\pm}0.01mm$ performed conducted by sand bottom circulation filtering method. Collected spats grew up to $3.1{\pm}0.8mm$ in 46 days, $6.6{\pm}1.8mm$ in 87 days, and $10.5{\pm}0.9mm$ in 114 days. The relative growth between SL and shell height (SH) was calculated to be SH = 0.8501SL + 0.0196 ($R^2=0.9987$) during the whole spat period. During spats rearing, they were suffered from one time of mass mortality at SL 3.1 mm, but 51,000 spats were finally survived with the rate of 39.2% at 114 days of spat rearing in indoor tank system.

2001년 8월 8일부터 12월 5일까지 말백합 대량 인공종묘 생산 실험을 실시하였다. 채란은 평균각장 $65.8{\pm}8.4mm$의 어미 100 개체로부터 공기노출과 수온상승자극 방법으로 채란 하였으며, 수정란에서 D형유생까지 발생 소요시간은 수온 $27^{\circ}C$에서 17시간 40분, 발생률은 6.1%, D형유생은 각장 $131.4{\pm}2.6{\mu}m$였다. D형유생은 4일간 사육하여 각장 $190.2{\pm}7.5{\mu}m$의 침착기 유생으로 성장하였고, 생존율은 48.1%였다. 이후 침착기 유생 130,000 개체를 저면 모래순환여과 방법으로 침착시켜 사육하였으며, 46일째 평균각장 $3.1{\pm}0.8mm$, 87일째 $6.6{\pm}1.8mm$, 그리고 114일째에 $10.5{\pm}0.9mm$로 성장하였다. 치패의 각장 (SL) 에 대한 각고(SH) 의 상대성장식은 SH = 0.8501SL + 0.0196 ($R^2=0.9987$)로 나타났다. 초기 침착치패인 각장 3.1 mm 이하에서 대량폐사가 일어났으며, 생존율은 사육 46일째 53.8%, 87일째 43.6%, 그리고 114일째에 51,000 개체가 생존하여 생존율 39.2%를 나타냈다.