International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Inhibitory Effect of the Culture Broth Extract of Aspergillus tamarii on Nitric Oxide Production and Its Antioxidative Activity

  • Lee, Ki Man (College of Pharmacy and Institute of Chronic Disease, Sahmyook University) ;
  • Lee, Geum Seon (College of Pharmacy and Institute of Chronic Disease, Sahmyook University) ;
  • Shim, Hong (College of Pharmacy and Institute of Chronic Disease, Sahmyook University) ;
  • Kim, Seung Hyun (College of Pharmacy and Institute of Chronic Disease, Sahmyook University) ;
  • Nam, Sung Hee (Department of Agricultural Biology, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kang, Tae Jin (College of Pharmacy and Institute of Chronic Disease, Sahmyook University)
  • Received : 2012.11.16
  • Accepted : 2012.11.28
  • Published : 2012.12.31
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Abstract

Many studies have explored suppression of aflatoxin produced by Aspergillus Genus. On the other hand, this study examined the inhibitory effect of the culture broth extract (CE) of A. tamarii obtained from dead silkworm on nitric oxide (NO) production and its antioxidative activity. The culture broth was extracted with EtOAc, dried, and then used in this experiment. As a result, CE did not show cytotoxicity on RAW 264.7 cells at any concentration. Moreover, CE suppressed lipopolysaccharide (LPS)-induced NO production of RAW 264.7 cells in a dose-dependent manner. The total phenol content according to the Folin-Dennis method, the antioxidative activity by DPPH, and the nitrate radical scavenging capacity of CE were increased in a dose-dependent manner. Thus, many of the phenolic compounds were considered to represent the antioxidative activity.

Keywords

References

  1. Chang LP, Lai YS, Wu CJ, Chou TC (2009) Liquid perfluorochemical inhibits inducible nitric oxide synthase expression and nitric oxide formation in lipopolysaccharide-treated RAW 264.7 macrophages. J Pharmacol Sci 111, 147-154. https://doi.org/10.1254/jphs.09043FP
  2. Choe SY, Yang KH (1982) Toxicological studies of antioxidants, butylated hydroxytoluene (BHT) and butlylated hydroxyanisole (BHA). Kor J Food Sci Technol 14, 283-288.
  3. Doan T, Melvold R, Viselli S, Waltenbaugh C (2008) Lippincott's illustrated reviews: Immunology. Lippincott Williams & Wilkins.
  4. Horn BW, Ramiez-Prado JH, Carbone I (2009) The sexual state of Aspergillus parasiticus. Mycologia 101, 275-280. https://doi.org/10.3852/08-205
  5. Huang SJ, Tsai SY, Lee YL, Mau JL (2006) Nonvolatile taste components of fruit bodies and mycelia of Cordyceps militaris. LWT 39, 577-583. https://doi.org/10.1016/j.lwt.2005.05.002
  6. Hwang PA, Chien SY, Chan YL, Lu MK, Wu CH, Kong ZL, Wu CJ (2011) Inhibition of lipopolysaccharide (LPS)-induced inflammatory responses by Sargassum hemiphyllum sulfated polysaccharide extract in RAW 264.7 macrophage cells. J Agric Food Chem 59, 2062-2068. https://doi.org/10.1021/jf1043647
  7. Kang KJ, Jeoung JH, Cho JI (2000) Inhibition of aflatoxin-producing fungi with antifungal compound produced by Bacillus subtilis. J Fd Hyg Safety 15, 122-127.
  8. Kim JW, Doo HS, Kwon TH, Kim YS, Shin DH (2011) Quality characteristics of Doenjang meju fermented with Aspergillus species and Bacillus subtilis during fermentation. Kor J Food Preserv 18, 297-306. https://doi.org/10.11002/kjfp.2011.18.3.397
  9. Kindt TJ, Goldsby RA, Osborne BA (2007) Kuby immunology. 6th ed. W.H. Freeman.
  10. Kumar V, Abbas AK, Fausto N, Michell RN (2007) Robbins basic pathology. 8th ed. England: Elsevier.
  11. Lee HO, Lee KH, Park NK, Jeong SI, Baek SH, Han DM (2000) Antibacterial effects of Sophora flavescens on Streptococcus mutans. Kor J Food Nutr 13, 539-546.
  12. Lee KM, Hong IP, Nam SH, Sung GB, Bae YH (2008) The cultural characteristics and antibacterial activities of Cordyceps militaris and Paecilomyces tenuipes. Kor J Appl Entomol 47, 479-486. https://doi.org/10.5656/KSAE.2008.47.4.479
  13. Liu WJH (2011) Traditional herbal medicine research methods. John Wiley & Sons.
  14. Martins HM, Almeida I, Marques M, Bernardo F (2008) Interaction of wild strains of Aspergilla with Aspergillus parasiticus ATCC15517 and aflatoxin production. Int J Mol Sci 9, 394-400. https://doi.org/10.3390/ijms9030394
  15. Nam SH, Hwang JS, Cho SY, Goo TW (1999) Genetic relationship of Cordyceps spp. based on internal transcribed spacer sequence of ribosomal DNA. Kor J Seri Sci 41, 174-179.
  16. Nam SH, Yoon CS, Jung IY, Ji SD, Cho SY, Han MS (2002) Occurrence and characteristics of other fungi in the artificial cultivation farms of Paecilomyces tenuipes. Kor J Seric Sci 44, 32-36.
  17. Samson RA, Evans HC, Latge JP (1988) Atlas of entomopathogenic fungi. Springer.
  18. Sung JM, Lee HK, Choi YS, Kim YY, Kim SH, Sung GH (1998) Classification of Cordyceps species based on protein banding pattern. Kor J Mycol. 26, 1-7.
  19. Whang HJ, Han WS, Yoon KR (2001) Quantitative analysis of total phenolic content in apple. Analytical Sci Technol 14, 377-383.