Journal of Soil and Groundwater Environment (한국지하수토양환경학회지:지하수토양환경)

Korean Society of Soil and Groundwater Environment (한국지하수토양환경학회)
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Monitoring of Geothermal Systems Wells and Surrounding Area using Molecular Biological Methods for Microbial Species

분자생물학적 방법을 이용한 지열시스템 관정 및 주변지역 미생물종 모니터링

  • Received : 2012.03.02
  • Accepted : 2012.12.07
  • Published : 2012.12.31
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Abstract

This study was conducted to monitor microbial species dynamics within the aquifer due to long term operation of geothermal heat pump system. The species were identified by molecular biological methods of 16S rDNA. Groundwater sample was collected from both open (S region) and closed geothermal recovery system (J region) along with the control. J measured and control as well as S measured found Ralstonia pickettii as dominant species at year 2010. In contrast, Rhodoferax ferrireducens was dominantly observed for the control of S. In 2011, Sediminibacterium sp. was universely identified as the dominant species regardless of the monitoring places and type of sample, i.e., measured or control. The difference in the dynamics between the measured and the control was not critically observed, but annual variation was more strikingly found. It reveals that possible environmental changes (e.g. ORP and DO) due to the operation of geothermal heat recovery system in aquifer could be more exceedingly preceded to differentiate annual variation of microbial species rather than positional differences.

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References

  1. Andersona, R.C. and Gardner, D.E., 1999, An Evaluation of the Wilt-Causing Bacterium Ralstonia solanacearum as a Potential Biological Control Agent for the Alien Kahili Ginger (Hedychium gardnerianum) in Hawaiian Forests, Biol. Control, 15(2), 89-96. https://doi.org/10.1006/bcon.1999.0705
  2. Bell, S.G., Yang, W., Yorke, J.A., Zhou, W., Wang, H., Harmer, J., Copley, R., Zhang, A., Zhou, R., Bartlam, M., Rao, Z., and Wong, L.-L., 2012, Structure and function of CYP108D1 from Novosphingobium aromaticivorans DSM12444: an aromatic hydrocarbon-binding P450 enzyme, Acta Crystallogr. Sect. DBiol. Crystallogr., D68, 277-291.
  3. Boutros, N., Gonullu, N., Casetta, A., Guibert, M., Ingrand D., and Lebrun1, L., 2002, Ralstonia pickettii Traced in Blood Culture Bottles, J. Clin. Microbiol., 40(7), 2666-2667. https://doi.org/10.1128/JCM.40.7.2666-2667.2002
  4. Finneran, K.T., Johnsen, C.V., and Lovley, D.R., 2003, Rhodoferax ferrireducens sp. nov., a psychrotolerant, facultatively anaerobic bacterium that oxidizes acetate with the reduction of Fe(III), Int. J. Syst. Evol. Microbiol., 53, 669-673. https://doi.org/10.1099/ijs.0.02298-0
  5. Gans, J., Wolinsky, M., and Dunbar, J., 2005, Computational improvements reveal great bacterial diversity and high metal toxicity in soil, Science, 309, 1387-1390. https://doi.org/10.1126/science.1112665
  6. Hashidoko, Y., Takakai, F., Toma, Y., Darung, U., Melling, L., Tahara, S., and Hatano, R., 2008, Emergence and behaviors of acid-tolerant Janthinobacterium sp. that evolves N2O from deforested tropical peatland, Soil Biol. Biochem., 40, 116-125. https://doi.org/10.1016/j.soilbio.2007.07.014
  7. Hiramatsu, J., Kataoka, M., and Nakata, Y., 2003, Propionibacterium acnes DNA detected in bronchoalveolar lavage cells from patients with sarcoidosis, Sarcoidosis Vasc. Diffus. Lung Dis., 20(3), 197-203.
  8. Jamieson, W.D., Pehl, M.J., Gregory G.A., and Orwin, P.M., 2009, Coordinated surface activities in Variovorax paradoxus EPS, BMC Microbiol., 9(124), 1-18. https://doi.org/10.1186/1471-2180-9-1
  9. Jo, Y.-J., Lee, J.-Y., Lim, S.-Y., and Hong, G.-P., 2009, A Review on Potential Effects of Installation and Operation of Ground Source Heat Pumps on Soil and Groundwater Environment, J. Soil & Groundwater Env., 14(3), 22-31.
  10. Jo, Y.-J., Lee, J.-Y., Kim, C.-G., and Han, J.-S., 2009, Effects of Grouts and Temperature Change on Microorganisms in Geothermal Heat Pump, J. Soil & Groundwater Env., 14(4), 22-31.
  11. KaSmpfer, P., Witzenberger, R., Denner, E.B.M., Busse, H.-J., and Neef, A., 2002, Sphingopyxis witflariensis sp. nov., isolated from activated sludge, Int. J. Syst. Evol. Microbiol., 52, 2029- 2034 https://doi.org/10.1099/ijs.0.02217-0
  12. Mattes, T.E., Alexander, A.K., Richardson, P.M., Munk, A.C., Han, C.S., Stothard, P., and Coleman, N.V., 2008, The Genome of Polaromonas sp. Strain JS666: Insights into the Evolution of a Hydrocarbon- and Xenobiotic-Degrading Bacterium, and Features of Relevance to Biotechnology, Appl. Environ. Microbiol., 74(20), 6405-6416. https://doi.org/10.1128/AEM.00197-08
  13. Micek, S.T., Lloyd, A.E., Ritchie, D.J., Reichley, R.M., Fraser, V.J., and Kollef, M.H., 2005, Pseudomonas aeruginosa Bloodstream Infection: Importance of Appropriate Initial Antimicrobial Treatment, Antimicrob. Agents Chemother., 49(4), 1306- 1311. https://doi.org/10.1128/AAC.49.4.1306-1311.2005
  14. Muyzer, G., De Waal, E.C., and Uitterlinden, A.G., 1993, Profiling of complex microbial populations by analysis of polymerase chain denaturing gradient gel electrophoresis reactionamplified genes coding for 16S rRNA, Appl. Environ. Microbio., 59(3), 695-700.
  15. Nakatsu, C.H., Hristova, K., Hanada, S., Meng, X.-Y., Hanson, J.R., Scow, K.M., and Kamagata, Y., 2006, Methylibium petroleiphilum gen. nov., sp. nov., a novel methyl tert-butyl ether-degrading methylotroph of the Betaproteobacteria, Int. J. Syst. Evol. Microbiol., 56, 983-989. https://doi.org/10.1099/ijs.0.63524-0
  16. Qu, J.-H. and Yuan, H.-L., 2008, Sediminibacterium salmoneum gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from sediment of a eutrophic reservoir, Int. J. Syst. Evol. Microbiol., 58, 2191-2194. https://doi.org/10.1099/ijs.0.65514-0
  17. Rappé, M.S. and Giovannoni, S.J., 2003, The uncultured microbial majority, Annu. Rev. Microbiol., 57, 369-394. https://doi.org/10.1146/annurev.micro.57.030502.090759
  18. Salinero, K.K., Keller, K., Feil1, W.S., Feil1, H., Trong, S., Di Bartolo, G., and Lapidus, A., 2009, Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB: indications of a surprisingly complex life-style and cryptic anaerobic pathways for aromatic degradation, BMC Genomics, 10(351).
  19. Sessitsch, A., Coenye, T., Sturz, A.V., Vandamme, P., Ait Barka, E., Salles, J.F., Van Elsas, J.D., Faure, D., Reiter, B. Glick, B.R., Wang-Pruski, G., and Nowak, J., 2005, Burkholderia phytofirmans sp. nov., a novel plant-associated bacterium with plantbeneficial properties, Int. J. Syst. Evol. Microbiol., 55, 1187-1192. https://doi.org/10.1099/ijs.0.63149-0
  20. Sowers, L.S., Epstein, C., York, K.P., Van Guilder, B., Jahangir, S., Steinberg, A., Stafford, L., Pierce, J., Brownhill J., and T. Solomon, T., 1997, Impact of a Large Scale Geothermal Project on its Surface and Subterranean Environment, Proceedings of the 7th International Conference on Thermal Energy Storage MEGASTOCK: Vol. 1 : 85-90.
  21. Takeda, M., Makita, H., Ohno, K., Nakahara, Y., and Koizumi, J.-I., 2005, Structural analysis of the sheath of a sheathed bacterium, Leptothrix cholodnii, Int. J. Biol. Macromol., 37, 92-98. https://doi.org/10.1016/j.ijbiomac.2005.09.002
  22. Ward, N., Larsen, O., Sakwa, J., Bruseth, L., Khouri, H., Durkin, A.S., Dimitrov, G., Jiang, L., Scanlan, D., Kang, K.H., Lewis, M., Nelson, K.E., Methe, B., Wu, M., Heidelberg, J.F., Paulsen, I.T., Fouts, D., Ravel, J., Tettelin, H., Ren, Q., Read, T., DeBoy, R.T., Seshadri, R., Salzberg, S.L., Jensen, H.B., Birkeland, N.K., Nelson, W.C., Dodson, R.J., Grindhaug, S.H., Holt, I., Eidhammer, I., Jonasen, I., Vanaken, S., Utterback, T., Feldblyum, T.V., Fraser, C.M., Lillehaug, J.R., and Eisen, J.A., 2004, Genomic Insights into Methanotrophy: The Complete Genome Sequence of Methylococcus capsulatus (Bath), PLoS. Biol., 2(10), 1616-1628.
  23. York, K.P., Sarwar Jahangir, Z.M.G., Solomon, T., and Stafford, L., 1998, Effects of a Large Scale Geothermal Heat Pump Installation on Aquifer Microbiota, In 2nd Stockton International Geothermal Conference, p. 8.

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