한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제41권12호
  • /
  • Pages.1686-1692
  • /
  • 2012
  • /
  • 1226-3311(pISSN)
  • /
  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
권호 표지
DOI QR코드

DOI QR Code

추출 방법이 개두릅 추출물의 항산화 활성에 미치는 영향

Antioxidative Activity of Kalopanax pictus Shoot Extracted Using Different Extraction Methods

  • 강경명 (대구가톨릭대학교 식품가공학과) ;
  • 노홍균 (대구가톨릭대학교 식품가공학과) ;
  • 박창수 (대구가톨릭대학교 식품가공학과) ;
  • 윤광섭 (대구가톨릭대학교 식품가공학과) ;
  • 홍주헌 (대구가톨릭대학교 식품가공학과) ;
  • 이신호 (대구가톨릭대학교 식품가공학과)
  • Kang, Kyoung-Myoung (Dept. of Food Service & Technology, Catholic University of Daegu) ;
  • No, Hong-Kyoon (Dept. of Food Service & Technology, Catholic University of Daegu) ;
  • Park, Chang-Su (Dept. of Food Service & Technology, Catholic University of Daegu) ;
  • Youn, Kwang-Sup (Dept. of Food Service & Technology, Catholic University of Daegu) ;
  • Hong, Joo-Heon (Dept. of Food Service & Technology, Catholic University of Daegu) ;
  • Lee, Shin-Ho (Dept. of Food Service & Technology, Catholic University of Daegu)
  • 투고 : 2012.08.20
  • 심사 : 2012.10.22
  • 발행 : 2012.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

개두릅 추출물의 항산화 활성을 증가시킬 수 있는 적정추출방법을 개발하기 위하여 상온교반, 초음파, 감압, 환류냉각, 환류냉각과 초음파 병행 추출법을 이용하여 추출한개두룹 추출물의 항산화 활성을 비교하였다. 추출수율은 환류냉각과 초음파 병행 추출물에서 높게 나타났다. 총 폴리페놀 및 총 플라보노이드의 함량 또한 환류냉각과 초음파 병행추출물이 각각 153.43 mg GAE/g, 62.53 mg RE/g으로 높았으며, 모든 추출물은 항산화 실험에서 농도 증가에 비례하여 활성이 증가하였다. 환류냉각과 초음파 병행 추출물은 추출농도 1,000 ${\mu}g/mL$에서 DPPH와 ABTS 라디칼 소거능(90.85%, 99.83%), 환원력(OD 0.95, 700 nm), 아질산염 소거능(55.46%), ferrous ion chelating 효과(45.12%)가 우수하거나 유사하였다.

The antioxidative activity of Kalopanax pictus shoots extracted using five different methods, stirrer extraction (SE), ultrasonification extraction (USE), vacuum extraction (VE), reflux extraction (RE), and reflux extraction after ultrasonification extraction (RUE), was evaluated to determine the most effective extraction method. RUE showed the highest yield of extract, and total polyphenol and flavonoid content. Additionally, the RUE extract showed higher antioxidative activity than that of the other extracts based on DPPH and ABTS free radical scavenging (90.85%, 99.83%), reducing power activity (OD 0.95, 700 nm), nitrite scavenging activity (55.46%), and ferrous ion chelating effect (45.12%).

키워드

참고문헌

  1. Branen AL. 1975. Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J Am Oil Chem Soc 52: 59-63. https://doi.org/10.1007/BF02901825
  2. Wang TC, Ti MC, Lo SC, Yang CC. 2007. Free radical-scavenging activity of aqueous extract of Pteris multifida Poiret. Fitoterapia 78: 248-249. https://doi.org/10.1016/j.fitote.2006.11.004
  3. Liu H, Qiu N, Ding H, Yao R. 2008. Polyphenols contents and antioxidant capacity of 68 Chinese harbals suitable for medical or food uses. Food Res Int 41: 363-370. https://doi.org/10.1016/j.foodres.2007.12.012
  4. Lee E, Choi MY, Park HJ, Cha BC, Cho SH. 1995. Chemical constituents and biological activity of Kalopanacis Cortex. Kor J Pharmacogn 26: 122-129.
  5. Park CH, Ahn SD, Jang BH, Ham SS. 1995. Explanation of herbs at hils and moors. Kangwon University publishing occupation, Chuncheon, Korea. p 102.
  6. Jun DH, Lee JT, Cheon SJ, Lee CE, Kim TH, Lee DH, Han JG, Kim SH. 2009. Polyphenol and anti-oxidant effects of Kalopanax septemlobus Koidz. leaf extracts. Korean J Plant Res 22: 343-348.
  7. Kim SH, Park YG, Jang YS, Han JG, Chung HG. 2007. Oxidative stress in the cell and antioxidant activity of Kalopanax pictus extracts. Mokchae Konghak 35: 126-134.
  8. Park HJ, Nam JH, Jung HJ, Kim WB, Park KK, Chung WY, Choi JW. 2005. In vivo antinociceptive antiinflamatory and antioxidative effects of the leaf and stem bark of Kalopanax pictus in rats. Kor J Pharmacogn 36: 318-323.
  9. Kim JH, Sung NY, Kwon SK, Jung PM, Choi JI, Yoon YH, Song BS, Yoon TY, Kee HJ, Lee JW. 2010. Antioxidant activity of stevia leaf extracts prepared by various extraction methods. J Korean Soc Food Sci Nutr 39: 313-318. https://doi.org/10.3746/jkfn.2010.39.2.313
  10. Park JH, Lee HS, Mun HC, Kim DH, Seong NS, Jung HG, Bang JK, Lee HY. 2004. Effect of ultrasonification process on enhancement of immuno-stimulatory activity of Ephedra sinica Stapf and Rubus coreanus Miq. Korean J Biotechol Bioeng 19: 113-117.
  11. Singleton VL, Joseph A, Rossi J. 1958. Colorimetry of total phenolics with phosphomolibdic-phosphotungsitc acid reagent. Am J Clin Nutr 68: 1474-1479.
  12. Abdel-Hameed ESS. 2009. Total phenolic contents and free radical scavenging activity of certain Egyptian Ficus species leaf samples. Food Chem 114: 1271-1277. https://doi.org/10.1016/j.foodchem.2008.11.005
  13. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  14. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radial cation decolorization assay. Free Radic Biol Med 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  15. Oyaizu M. 1986. Studies on products of browning reaction prepared from glucose amine. Jpn J Nutr 44: 307-315. https://doi.org/10.5264/eiyogakuzashi.44.307
  16. Kato H, Lee IE, Chuyen NV, Kim SB, Hayase F. 1987. Inhibition of nitrosamine formation by non-dialyzable melanoidins. Agric Biol Chem 51: 1333-1338. https://doi.org/10.1271/bbb1961.51.1333
  17. Marklund S, Marklund G. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 469-474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  18. Yen GC, Duh PD, Tsai HL. 2002. Antioxidant and pro-oxidant properties of ascorbic acid and gallic acid. Food Chem 79: 307-313. https://doi.org/10.1016/S0308-8146(02)00145-0
  19. Park JH, Lee HS, Mun HC, Kim DH, Seong NS, Jung HG, Bang JK, Lee HY. 2004. Improvement of anticancer activation of ultrasonificated extracts from Acanthopanax senticosus Harms, Ephedra sinica Stapf, Rubus coreanus Miq. and Artemisia capillaris Thunb. Korean J Medicinal Crop Sci 12: 273-278.
  20. Beecher GR. 2003. Overview of dietary flavonoids: nomenclature, occurrence and intake. J Nutr 133: 3248S-3254S. https://doi.org/10.1093/jn/133.10.3248S
  21. Chung HJ. 1999. Antioxidative effect of ethanolic extracts of some tea materials on red pepper seed oil. J Korean Soc Food Sci Nutr 28: 1316-1320.
  22. Yu JS, Woo KS, Hwang IG, Chang YD, Jeong HJ, Lee CH, Jeong HS. 2008. Quality characteristics of Chrysanthemum indicum L. flower tea in relation to the number of pan-firing. J Korean Soc Food Sci Nutr 37: 647-652. https://doi.org/10.3746/jkfn.2008.37.5.647
  23. Hong MJ, Lee GD, Kim HK, Kwon JH. 1998. Changes in functional and sensory properties of Chicory roots induced by roasting processes. Korean J Food Sci Technol 30: 413-418.
  24. Choi JH, Oh SK. 1985. Studies on the anti-aging action of Korean ginseng. Korean J Food Sci Technol 17: 506-515.
  25. Kang MH, Park CG, Cha MS, Seong NS, Chung HK, Lee JB. 2001. Component characteristics of each extract prepared by different extract methods from by-products of Glycyrrhizia uralensis. J Korean Soc Food Sci Nutr 30: 138-142.
  26. Yen GC, Chen HY. 1995. Antioxidant activity of various tea extracts in relation to their antimutagenicity. J Agric Food Chem 43: 27-32. https://doi.org/10.1021/jf00049a007
  27. Osawa T. 1994. Novel natural antioxidant for utilization in food and biological system. In Postharvest Biochemistry of Plant Food Material in the Tropics. Uritani I, Garcia VV, Mendoza EM, eds. Japan Scientific Societies Press, Tokyo, Japan. p 241-251.
  28. Na GM, Han HS, Ye SH, Kim HK. 2004. Physiological activity of medicinal plant extracts. Kor J Food Preserv 11: 388-393.
  29. Kim SM, Cho YS, Sung SK. 2001. The antioxidant ability and nitrite scavenging ability of plant extracts. Korean J Food Sci Technol 33: 626-632.
  30. Yamada T, Yamamoto M, Tamura A. 1978. Studies on the formation of nitrosamines VII; The effects of some polyphenols on nitrosation of diethylamine. J Food Hyg Soc Jpn 19: 224-229. https://doi.org/10.3358/shokueishi.19.224
  31. Lim JD, Yu CY, Kim MJ, Yun SJ, Lee SJ, Kim NY, Chung IM. 2004. Comparison of SOD activity and phenolic compound contents in various Korean medicinal plants. Korean J Medicinal Crop Sci 12: 191-202.
  32. An BJ, Lee JT. 2002. Studies on biological activity from extract of Crataegi Fructus. Kor J Herbology 17: 29-38.
  33. Yoo MY, Kim SK, Yang JY. 2004. Characterizatio of an antioxidant from sporophyll of Undaria pinnatifida. Kor J Microbiol Biotechnol 32: 307-311.
  34. Graf E, Eaton JW. 1990. Antioxidant functions of phytic acid. Free Radic Biol Med 8: 61-69. https://doi.org/10.1016/0891-5849(90)90146-A
  35. Woo JH. 2008. Antioxidant substance and biological activity of compositae species. MS Thesis. Chungbuk Natl University, Cheongju, Korea. p 42-44.
  36. Kim HK, Kwon, YJ, Kim YE, Nahmgung B. 2004. Changes of total polyphenol content and antioxidant activity of Aster scaber Thunb extracts with different microwave-assisted extraction conditions. Kor J Food Preserv 11: 88-93.
  37. Kim JH, Kim DH, You JH, Kim CH, Kwon MC, Seong NS, Lee SE, Lee HY. 2005. Immune-regulatory activities of various fractions from Ehpedrae Sinica STAPF, Rubus Coreanus Miq. and Angelica gigas Nakai extracts with ultrasonification. Korean J Medicinal Crop Sci 13: 161-170.
  38. Kim DH, Kim HJ, Chung BW. 2006. Extraction of anti-oxidative substance from Haematococcus pluvialis using ultrasonification. J Eng Res 37: 79-86.
  39. Yasui K, Tuziuti T, Iida Y. 2005. Dependence of the characteristics of bubbles on types of sonochemical reactors. Ultrason Sonochem 12: 43-51. https://doi.org/10.1016/j.ultsonch.2004.06.003

피인용 문헌

  1. L. in Jeju vol.60, pp.4, 2017, https://doi.org/10.3839/jabc.2017.048
  2. 염수초 포제법에 따른 두충의 항산화 및 항염증 활성 변화 비교연구 vol.27, pp.2, 2017, https://doi.org/10.18325/jkmr.2017.27.2.29
  3. Quality Characteristics and Antioxidant Activities of Liquor Containing Rose, Camellia, and Cockscomb vol.28, pp.2, 2012, https://doi.org/10.17495/easdl.2018.4.28.2.101
  4. FDY003의 항산화활성 및 표준화 연구 vol.40, pp.6, 2019, https://doi.org/10.22246/jikm.2019.40.6.1112