Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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The Effect of Opuntia humifusa Seed Extracts on Platelet Aggregation and Serum Lipid Level in Ovariectomized Rats

천년초 씨 추출물이 난소를 절제한 흰쥐의 혈소판 응집과 혈청 내 지질 조성에 미치는 영향

  • Hwang, Hyun Jung (Department Food and Nutrition, Silla University) ;
  • Kang, Min Sook (Department Food and Nutrition, Silla University) ;
  • Kim, Bo Kyung (Department Food and Nutrition, Silla University) ;
  • Jung, Bok-Mi (Major in Food Science and Nutrition, Chonnam National University) ;
  • Kim, Mihyang (Department Food and Nutrition, Silla University)
  • 황현정 (신라대학교 식품영양학과) ;
  • 강민숙 (신라대학교 식품영양학과) ;
  • 김보경 (신라대학교 식품영양학과) ;
  • 정복미 (전남대학교 식품영양학과) ;
  • 김미향 (신라대학교 식품영양학과)
  • Received : 2012.11.04
  • Accepted : 2012.12.16
  • Published : 2012.12.30
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Abstract

Postmenopausal women are at an increased risk of developing coronary artery disease, primarily due to dyslipidemia that accompanies the loss of estrogen secretion. This study was performed to investigate the effects of Opuntia humifusa seed extracts on blood flow and serum lipid level in ovariectomized rats. Twenty-eight 9-week old female Sprague-Dawley rats were randomly assigned to four groups as sham-operated rats (SHAM), ovariectomized rats (OVX-CON), and ovariectomized rats that were treated with Opuntia humifusa seed extracts (OVX-OHS 2% and OVX-OHS 6%). The diets were fed to the rats for 7 weeks after operation. Serum total cholesterol and triglyceride contents decreased in the SHAM group compared to the OVX-CON group. Seven weeks of feeding of Opuntia humifusa seed extracts resulted in significant (p<0.05) lowering of serum triglyceride and a decreasing tendency in total cholesterol levels. The level of HDL-cholesterol in serum increased significantly by feeding diets containing the 2% and 6% Opuntia humifusa seed hot-water extract (p<0.05). Blood passage times were shorter in the Opuntia humifusa seed extract-supplemented groups than in the untreated group (OVX-CON). The platelet aggregation ability was lower in groups treated with Opuntia humifusa seed extracts than in the OVX-CON group. These results suggest that Opuntia humifusa seed extracts may have benefits in improving metabolic syndrome in menopausal women.

Estrogen의 감소는 다양한 생리적 변화를 초래하며 특히 심혈관계 질환을 빠르게 진행시킨다고 보고되고 있다. 본 연구에서는 실험동물인 흰쥐의 난소를 절제하여 인위적으로 갱년기를 유도한 후 선인장류인 천년초 씨 추출물의 혈중 지질 함량 및 혈류 개선에 대한 영향을 검토하였다. 7주 동안 천년초 씨 추출물을 섭취한 OHS 2%, OHS 6%군은 체중이 다소 증가하여 체중감소에는 효과를 나타내지 않았지만 섭취 농도가 증가 할수록 식이효율은 낮아져 비만조절 효과의 가능성이 기대된다. 총 콜레스테롤 함량은 천년초 추출물 투여로 인한 유의성 있게 감소하는 결과가 나타나지 않았으나, 혈청 중 중성지방 함량의 경우 천년초 씨 추출물이 2%와 6% 함유된 사료를 섭취시킨 OVX-OHS 2%군 및 OVX-OHS 6%군의 경우, OVX-CON군과 비교하여 중성지방 함량이 유의적으로 감소하는 결과가 나타났다. 혈청 중 LDL-콜레스테롤 함량은 OVX-CON군의 경우 SHAM군에 비해 유의적으로 증가하였으나, 천년초 추출물 투여군인OVX-OHS 2%군과 OVX-OHS 6%군의 경우 OVX-CON군에 비해 유의적으로 감소하는 결과가 나타났다. 동맥경화지수(atherogenic inde, AI)는 난소 절제한 OVX-CON군에 비하여 난소 절제 후 천년초 씨 추출물 첨가식이를 섭취한 OVX-OHS 6%군에서 유의적으로 감소되었다. 또한 심혈관위험지수를 나타내는 CRF역시 천년초 씨 추출물 첨가식이 섭취로 인해 감소하는 결과를 나타내었으며, 특히 OVX-OHS 6%군에서 유의적으로 감소하는 경향을 나타냈었다. 또한 천년초 씨 추출물을 섭취로 인해 난소절제군과 비교하여 혈류 흐름을 개선시키고 혈소판 응집을 저해하는 경향을 나타내었다. 따라서 천년초 씨 추출물이 갱년기 여성의 심혈관계 질환에 유효한 효과를 나타낼 것으로 사료된다.

Keywords

References

  1. Anderson, J. W., Johnstone, B. M. and Cook-newell, M. E. 1995. Meta-analysis of the effects of soy protein intake on serum lipids. N. Engl. J. Med. 33, 276-282.
  2. Badimon, L, Badimon, J. J., Penny, W., Webster, M. W., Chesebro, J. H. and Fuster, V. 1992. Endothelium and atherosclerosis. J. Hypertens. 10, 43-50. https://doi.org/10.1097/00004872-199207001-00007
  3. Belchetz, P. E. 1994. Hormonal treatment of postmenopausal women. N. Engl. J. Med. 14. 1062-1071.
  4. Bonithon-Kopp, C, Scarabin, P. Y., Darne, B., Malmejac, A. and Guize, L. 1990. Menopause-related changes in lipoproteins and some other cardiovascular risk factors. Int. J. Epidemiol. 19, 42-48. https://doi.org/10.1093/ije/19.1.42
  5. Campagnoli, C., Biglia, N., Peris, C. and Sismondi, P. 1995. Potential impact on breast cancer risk of circulating insulin-like growth factor I modifications induced by oral HRT in menopause. Gynecol. Endocrinol. 9, 67-74 https://doi.org/10.3109/09513599509160194
  6. Choi, J. H. 2011. Structural analysis of anti-metastatic polysaccharides isolated from Opuntia humifusa. M. S. Thesis, Graduate School of Kyonggi University, Korea.
  7. Clarkson, T. B. 2002. Soy phytoestrogens a cardiovascular disease. J. Nutr. 132, 566-569.
  8. Gale, S. K. and Sclakani, A. 1977. Comparison of ovarian and hypothalamic obesity syndromes in the female rat: effect of diet palatability on food intake and body weight. J. Comp. Physiol. Psychol. 91, 381-392. https://doi.org/10.1037/h0077328
  9. Gavey, D. A. 1986. The menopause and climacteric. In Whitfield, C. R. (ed.), Dewhurst's text-book of obstetrics and gynecology for postgraduates. 4th eds., Oxford : Blackwell scientific publications, 646-676.
  10. Han, I. H., Lee, K. A. and Byoun, K. E. 2007. Antioxidant activity of Korean cactus (Opuntia humifusa) and the quality characteristics of cookies with cactus powder added. Korean J. Food Cookery Sci. 23, 443-451.
  11. Hwang, H. J., Jung, B. M. and Kim, M. H. 2012. ROS scavenging effect and cell viability of Opuntia humifusa extract on osteoblastic MC3T3-E1 cells. J. Life Sci. 21, 1752-1760. https://doi.org/10.5352/JLS.2011.21.12.1752
  12. Jeon, M. H. 2011. Effect of Hijkia fusiforme fractions on proliferation and differentiation in osteoblastic MC3T3-E1 cells. J. Life Sci. 21, 300-308. https://doi.org/10.5352/JLS.2011.21.2.300
  13. Ji, S. E. 1983. Analysis of the relationship between middle- aged woman's attitudes toward middle adulthood developmental changes and their self-reported climacteric symptoms. M.S. Thesis, Graduate School of Yonsei University, Korea.
  14. Kim, D. J., Jung, J. H., Kim, S. G., Lee, H. K., Lee, S. K., Hong, H. D., Lee, B. Y. and Lee, O. H. 2011. Antioxidants and anti-obesity activities of hot water and ethanolic extracts from Cheonnyuncho (Opuntia humifusa). Korean J. Food Preserv. 18, 366-373. https://doi.org/10.11002/kjfp.2011.18.3.366
  15. Kim, S. G. 2011. Effects of Cheonnyuncho (Opuntia humifusa) extracts on the inhibition of adipocyte differentiation and the reduction of reactive oxygen species. D.S. Thesis, Kongju University, Korea.
  16. Kim. S. Y. 2003. Studies on the separation of antioxidative and anti-microbial compounds of Korean perennial cactus Cheonnyuncho. Ph D Dissertation, Hoseo University, Korea.
  17. Kim, Y. K., Jeon, M. H., Park, Y. S., Hwang, H. J., Lee, S. H., Kim, S. G., Kim, Y. Y. and Kim, M. H. 2010. The Effect of Ecklonia stolonifera extracts on blood flow and serum lipid level in ovariectomozed rats. J. Life Sci. 20, 1851-1858. https://doi.org/10.5352/JLS.2010.20.12.1851
  18. Lee, H. S. 2001. Experiences of postmenopausal women about hormone replacement therapy. M.S. Thesis, Graduate School of Chungang University, Korea.
  19. Lee, S. S. and Park, J. H. 1989. Long-term effect of ovariectomy on body composition. Korean J. Nutr. 22, 102-107.
  20. Levine, G. N., Keaney, J. F. and Vita, J. A. 1995. Cholesterol reduction cardiovascular disease; Clinical benefits and possible mechanisms. N. Engl. J. Med. 33, 512-521.
  21. Lisa, K. 2009. Mechanisms of platelet activation: Need for new strategies to protect against platelet-mediated atherothrombosis. Thromb Haemost. 2, 248-257.
  22. Manolio, T. A., Pearson, T. A., Wenger, N. K., Barrett- Connor, E., Opayne, G. H. and Harlan, W. R. 1992. Cholesterol and heart disease in older persons and women. Review of an NHLBI workshop. Ann. Epidemiol. 2, 161-176. https://doi.org/10.1016/1047-2797(92)90051-Q
  23. Mook, D. G., Kenney, N. J., Roberts, S. and Nussbaum, A. I. 1972. Ovarian-adrenal interactions in regulation of body weight by female rats. J. Comp. Physiol. Psychol. 81, 192-211.
  24. Mueller, K. and Hsiao, S. 1980. Estrus and ovariectomy-induced body weight changes: evidenced for two estrogenic mechanisms. J. Comp. Physiol. Psychol. 5, 1126-1134.
  25. Park, M. K., Lee, Y. J. and Kang, E. S. 2005. Hepatoprotective effect of Cheonnyuncho (Opuntia humifusa) extract in rats treated carbon tetrachloride. J. Korean Food Sci. Technol. 37, 822-826.
  26. Park, Y. S. and Kim, M. H. 2012. The effect of eisenia bicyclis extracts on antioxidant activity and serum lipid level in ovariectomized rats. J. Life Sci. 22, 1407-1414. https://doi.org/10.5352/JLS.2012.22.10.1407
  27. Pines, A. 1991. The effects of hormone replacement therapy in normal postmenopausal women : measurements of Doppler-derived parameters of aortic flow. Am. J. Obstet. Gynecol. 164, 806-812. https://doi.org/10.1016/0002-9378(91)90520-2
  28. Riggs, B. L., Wahner, H. W., Dunn, W. L., Mazess, R. B., Offord, K. P. and Melton, L. J. 1981. Differential changes in BMD of the appendicular and axial skeleton with aging: relationship to spinal osteoporosis. J. Clin. Invest. 67, 328-335. https://doi.org/10.1172/JCI110039
  29. Rossouw, J .E., Anderson, G. L., Prentice, R. L., LaCroix, A. Z., Kooperberg, C., Stefanick, M. L., Jackson, R. D., Beresford, S. A., Howard, B. V., Johnson, K. C., Kotchen, J. M. and Ockene, J. 2002. Risks and benefits of estrogen plus progestin in healthy postmenopausal women: principal results From the Women's Health Initiative randomized controlled trial. JAMA 288, 321-333. https://doi.org/10.1001/jama.288.3.321
  30. Schemmel, R. A. and Teague, R. T. 1982. Obesity in Osborne mendel and S 4B/P1 rats : effects of sucrose solution, castration and treatment with estradiol or insulin. Am. J. Physiol. 243, 347-352.
  31. Shimizu, H. and Shimomura, Y. 1992. Colonic temperature was not changed in the development of obesity after ovariectomy. Exp. Clin. Endocrinol. 2, 99-101.
  32. Tesfamariam, B. 1994. Free radicals in diabetic endothelial cell dysfunction. Free Rad. Biol. Med. 16, 383-391. https://doi.org/10.1016/0891-5849(94)90040-X
  33. Wolfgang, W., Huberus, J., Tamara, B., Alexander, S., Volker, C., Christoph, G. and Dana, S. W. 2003. Phytoestrogen: endocrine disrupters or replacement for hormone replacement therapy. Maturitas 4, 1-12.
  34. Yamazaki, I. and Yamaguchi, H. 1989. Characteristics of an ovariectomized osteogenic rat model. J. Bone Miner. Res. 4, 13-22.
  35. Yoon, J. A. and Son, Y. S. 2009. Effects of fruits and stems of Opuntia ficus-indica on blood glucose and lipid metabolism in streptozotocin-induced diabetic rats. J. Korean Soc. Food Sci. Nutr. 38, 146-153. https://doi.org/10.3746/jkfn.2009.38.2.146
  36. Yoon, J. A. and Son, Y. S. 2009. Effects of Opuntia ficus- indica complexes B (OCB) on blood glucose and lipid metabolism in streptozotocin-induced diabetic rats. Korean J. Food Nutr. 22, 48-56.
  37. Yoon, J. A., Hahm, S. W., Park, J. and Son, Y. S. 2009. Total polyphenol and flavonoid of fruit extract of Opuntia humifusa and its inhibitory effect on the growth of MCF-7 human breast cancer cells. J. Korean Soc. Food Sci. Nutr. 38, 1679-1684. https://doi.org/10.3746/jkfn.2009.38.12.1679
  38. Zeher, A. M., Schachlinger, V. and Hohnlosr, S. H. 1994. Coronary atherosclerotic wall thickening and vascular reactivity in humans: Elevated high-density lipoprotein levels ameliorate abnormal vasoconstriction in early atherosclerosis. Circulation 25, 2525-2532.

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