Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antioxidant Activity and Grain Properties of Colored Rice Derived from Insertional Mutagenesis Progenies

벼 종피색 변이체에 대한 항산화 활성 분석과 미립특성

  • Yi, Gihwan (Department of Farm Management, College of Agriculture & Life Science, Kyungpook National University) ;
  • Lee, Hyun-Suk (Division of Plant Biosciences, School of Applied Biosciences, Kyungpook National University) ;
  • Sohn, Jae-Keun (Division of Plant Biosciences, School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Kyung-Min (Division of Plant Biosciences, School of Applied Biosciences, Kyungpook National University)
  • 이기환 (경북대학교 농산업학과) ;
  • 이현숙 (경북대학교 식물생명과학전공) ;
  • 손재근 (경북대학교 식물생명과학전공) ;
  • 김경민 (경북대학교 식물생명과학전공)
  • Received : 2012.09.12
  • Accepted : 2012.12.14
  • Published : 2012.12.30
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Abstract

This study examined the antioxidant activity of the dark purple rice seeds from the rice line, MGI079, derived from insertional mutagenesis. The contents of polyphenolic compounds were 1.3 and 1.9-fold higher in the MGI079-2-1 and MGI079-2-6 rice lines than in the donor cultivar MGI079. Flavonoid contents were 6.4-fold higher in the MGI079-2-1 line. The MGI079-2-1 line showed a 24.4-fold higher activity in DPPH free radical scavenging compared to the MGI079 line. The anthocyanin content of the MGI079-2-6 line was more than 106.4-fold higher than the MGI079 line and 1.4-fold higher than the Heugnam line. Anthocyanin content in colored rice grains was negatively correlated with Hunter's L, a, and b values, with the correlation coefficients of $-5.64^{**}$, $5.21^{**}$ and -1.15, respectively. The grain length/width of a mutant of MGI079 segregated to a medium and bold type compared to the medium type of MGI079. However, the 1,000 grain weight was decreased to 13.6~19.6 g compared to 19.8 g for MGI079. Amylose content of the endosperm was 5.6~23.8% higher than in the MGI079 line. The grain of mutants of MGI079 was distinguished by its starch characteristics. The higher antioxidant activity of the MGI079-2-1 and MGI079-2-6 lines indicated functional characteristics associated with high-value resources, so future breeding should focus on the development of pigments in colored rice in new varieties.

벼(Oryza sativa L.)에서 Insertional 유래 MGI079 돌연변이 계통의 자색미에 대한 항산화 활성을 보기 위하여 폴리페놀, 플라보노이드, DPPH radical, 안토시아닌 및 색차계를 이용하여 분석하였다. 폴리페놀 함량에서 MGI079-2-1와 MGI079-2-6은 MGI079보다 1.3배에서 1.9배 이상 증가되었고, 플라보노이드에는 MGI079-2-1 MGI079보다 6.4배 이상 증가되었다. DPPH 자유기에는 MGI079-2-1이 MGI079보다 24.4배 이상 항산화력이 증대되었다. 안토시아닌 성분에는 MGI079- 2-6은 MGI079보다 106.4배 이상 증가되었으며 흑남에 비해서는 1.4배 증가하였다. 색차계에서 Hunter's 값(L, a, b)은 안토시아닌($-5.64^{**}$, $5.21^{**}$와 -1.15)의 L, a에서 유의성이 있는 것으로 보였다. MGI079 돌연변이 계통의 미립 장폭비는 MGI079에 비해서 medium과 bold type으로 분리되었으며, 천립중은 MGI079 (19.8 g)보다 돌연변이 계통들이 13.6~19.6 g으로 감소하였다. 아밀로스는 MGI079 보다 5.6~23.8% 증가하였고, 배유의 전분립 형태는 비정형 전분립 특성 등 차이를 보였다. Insertional 유래 MGI079 돌연변이 계통들의 항산화 활성 분석에서 MGI079-2-1와 MGI079-2-6은 MGI079와 흑남보다 항산화력이 높은 기능적 가치로 미립특성과 관련하여 색소 자원의 개발뿐만 아니라 신품종 육성에 필요한 예비품종으로 실용화가 기대된다.

Keywords

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