사상체질의학회지 (Journal of Sasang Constitutional Medicine)

  • 제24권4호
  • /
  • Pages.75-83
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  • 2012
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  • 1226-4075(pISSN)
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  • 2287-786X(eISSN)
사상체질의학회 (The Society Of Sasang Constitutional Medicine)
권호 표지
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태음인(太陰人) 열다한소탕(熱多寒少湯)이 3T3-L1 지방전구세포(脂肪前驅細胞) 분화(分化)에 미치는 영향(影響)

Effects of Yeoldahanso-tang, a Sasang Constitutional Herbal Prescription for Taeeumin, on the Adipogenesis in 3T3-L1 Cells

  • 유새롬 (한국한의학연구원 한약기초연구그룹) ;
  • 정수진 (한국한의학연구원 한약기초연구그룹) ;
  • 신현규 (한국한의학연구원 한약기초연구그룹)
  • Yoo, Sae-Rom (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Jeong, Soo-Jin (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
  • 투고 : 2012.10.08
  • 심사 : 2012.12.17
  • 발행 : 2012.12.31
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초록

Objectives : Although Yeoldahanso-tang (YDHST) has been widely used for treatment of obesity and its related diseases such as hyperinsulinemia and hypertension for Taeeumin, no scientific evidence has reported yet to support its ability to work against these metabolic disorders. Our study was aimed to investigate the anti-obesity effect of YDHST extract on the cellular differentiation of 3T3-L1 preadipocytes into adipocytes. Methods : 3T3-L1 preadipocytes were differentiated into adipocytes by adding insulin, dexamethasone and 3-isobutyl-1-methylxanthine (IBMX) for 8 days in the absence or presence of YDHST extract. Anti-obesity effects of YDHST extract were evaluated by Oil Red O staining, glycerol-3-phosphate dehydrogenase (GPDH) activity, triglyceride contents, and leptin production. Results : YDHST extract remarkably prevented lipid accumulation with no cytotoxicity in the differentiated 3T3-L1 cells. In addition, YDHST extract decreased contents of triglyceride 3T3-L1 adipocytes. Consistently, YDHST extract caused a significant inhibition of GPDH activity and leptin production in a dose-dependent manner. Conclusions : Our findings suggest that Sasang constitutional herbal formula YDHST for Tae-eumin has anti-obesity activity by regulation of the adipogenesis process in vitro. Additional study will be required to further confirm the inhibitory effect on adipocyte differentiation by using in vivo animal model.

키워드

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피인용 문헌

  1. Review of Studies on Taeumin Yeoldahanso-tang since 2000 vol.26, pp.1, 2014, https://doi.org/10.7730/JSCM.2014.26.1.098
  2. Protective effect of yeoldahanso-tang on radiation-induced oral mucositis in mice vol.16, pp.1, 2016, https://doi.org/10.1007/s13596-015-0211-9