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Testing microsatellite loci and preliminary genetic study for Eurasian otter in South Korea

  • Received : 2012.07.04
  • Accepted : 2012.08.24
  • Published : 2012.08.30

Abstract

We used a non-invasive technique with microsatellite primers to investigate genetic variation among Eurasian otters Lutra lutra in eastern South Korea. We collected twenty two otter spraints in January and six in August 2008. We used spraints from five dead otters from five different river systems for the present genetic analysis. We extracted DNA from 20 spraints from the January sample. Ten microsatellite primers (Lut435, Lut453, Lut457, Lut604, Lut615, Lut701, Lut715, Lut717, Lut733, and Lut832) for Eurasian otters were tested, and four loci were successfully amplified for further analyses. The results of genotyping the otter population with microsatellite loci lead to the identification of 9 individuals from the Ungokcheon Stream. The Ungokcheon population also showed a genetic structure represented by the Hardy-Weinberg equilibrium.

Keywords

References

  1. Brown, R.W., M.J. Lawrence and J. Pope. 1992. Animals: Tracks, Trails & Signs. Octopus Publishing group, London. 320p.
  2. Dallas, J.F. and S.B. Piertney. 1998. Microsatellite primers for the Eurasian otter. Molecular Ecology 7:1248-1251.
  3. Excoffier, L., G. Laval and S. Schneider. 2005. Arlequin Ver. 3.0: an integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1:47-50.
  4. Folmer, O., M. Black, W. Hoeh, R. Lutz and R. Vrijenhoek. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3:294-299.
  5. Goldstein, D. and C. Schlotterer. 1999. Microsatellites. Oxford University Press, Oxford. 343p.
  6. Hajkova, P., B. Zemanova, J. Bryja, B. Hajek, K. Roche, E. Tkadlec and J. Zima. 2006. Factors affecting success of PCR amplification of microsatellite Loci from otter faeces. Molecular Ecology Notes 6:559-562. https://doi.org/10.1111/j.1471-8286.2006.01269.x
  7. Imaizumi, Y. and M. Yoshiyuki. 1989. Taxonomic status of the Japanese otter (Carnivora, Mustelidae), with a description of a new species. Bulletin of the National Science Museum, ser. A (Zoology) 15:177-188.
  8. Jo, Y., B. Choi, C. Won and J. Kim. 2006. Ecology of otters and utilization of artificial holts in the ecosystem conservation region of the Seomjin River. Korean Journal of Environmental Biology 24:160-165.
  9. Jung, J., K.H. Song, E. Lee and W. Kim. 2009. Mitochondrial genetic variations in fishes of the genus Carassius from South Korea: proximity to northern China rather than Japanese Islands? Hydrobiologia 635:95-105. https://doi.org/10.1007/s10750-009-9866-x
  10. Kruuk, H. 1995. Wild otters: Predation and Populations. Oxford University press, Oxford. 304p.
  11. Laszki, J., A. Hidas, K. Szentes, T. Revay, I. Lehoczky and S. Weiss. 2008. Relative spraint density and genetic structure of otter (Lutra lutra) along the Drava River in Hungary. Mammalian Biology 73:40-47.
  12. Librado, P. and J. Rozas. 2009. DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451-1452. https://doi.org/10.1093/bioinformatics/btp187
  13. Macdonald, S.M. and C.F. Mason. 1987. Seasonal marking in an otter population. Acta Theriologica 32:449-462. https://doi.org/10.4098/AT.arch.87-31
  14. Mason, C.F. and S.M. Macdonald. 1986. Otters: Ecology and Conservation. Cambridge University Press, Cambridge. 236p.
  15. Palumbi, S.R., A. Martin, S. Romano, W.O. McMillan, L. Stice and G. Grabowski. 1991. The simple fool's guide to PCR, v.2.0. University of Hawaii, Honolulu. 45p.
  16. Reuther, C., D. Dolch, R. Green, J. Jahrl, D. Jefferies, A. Krekemeyer, M. Kucerova, A.B. Madsen, J. Romanowski, K. Roche, J. Ruiz-Olmo, J. Teubner and A. Trindade. 2000. Surveying and Monitoring Distribution and Population Trends of the Eurasian Otter. GN-Gruppe Naturschutz GmbH Sudendorfallee, Hankensbuttel. 149p.
  17. Sugimoto, T., J. Nagata, V.V. Aramilev and D.R. McCullough. 2012. Population size estimation of Amur tigers in Russian Far East using noninvasive genetic samples. Journal of mammalogy 93:93-101. https://doi.org/10.1644/10-MAMM-A-368.1
  18. Taberlet, P., L.P. Waits and G. Luikart. 1999. Noninvasive genetic sampling: look before you leap. TREE 14:323-327.
  19. Wagner, R.S., M.P. Miller, C.M. Crisafulli and S.M. Haig. 2005. Geographic variation, genetic structure, and conservation unit designation in the Larch Mountain salamander (Plethodon larselli). Canadian Journal of Zoology 83:396-406. https://doi.org/10.1139/z05-033
  20. Wasser, S.K., C.S. Houston, G.M. Koehler, G.G. Cadd and S.R. Fain. 1997. Techniques for application of faecal DNA methods to field studies of Ursids. Molecular Ecology 6:1091-1097. https://doi.org/10.1046/j.1365-294X.1997.00281.x
  21. Wison, D.E. and D.M. Reeder. 2005. Mammal species of the world. Johns Jopkins University press, Baltimore. 2142p.
  22. Won, C. and K.G. Smith. 1999. History and current status of mammals of the Korean Peninsula. Mammal Review 29:3-36. https://doi.org/10.1046/j.1365-2907.1999.00034.x

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