한국식품영양학회지 (The Korean Journal of Food And Nutrition)

  • 제25권4호
  • /
  • Pages.990-998
  • /
  • 2012
  • /
  • 1225-4339(pISSN)
  • /
  • 2287-4992(eISSN)
한국식품영양학회 (The Korean Society of Food and Nutrition)
권호 표지
DOI QR코드

DOI QR Code

늙은 호박, 율무, 꾸지뽕잎 혼합조성물이 고지방식이를 급여한 흰쥐의 내장지방, 배변량 및 혈청지질농도에 미치는 영향

Effects of Cucurbita moschata, Adlay Seed, and Cudrania tricuspidata Leaf Mixed-powder Diet Supplements on the Visceral Fat, Fecal Amount, and Serum Lipid Levels of the Rats on a High-Fat Diet

  • 투고 : 2012.11.07
  • 심사 : 2012.12.07
  • 발행 : 2012.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

고지방식이(D12492)를 5주 동안 흰쥐에게 급여한 다음 고지방식이에 늙은 호박(Cucurbita moschata), 율무(adlay seed), 꾸지뽕(Cudrania tricuspidata) 분말조성물(1:1:1) 첨가식이를 5주 동안 급여한 결과는 다음과 같다. 실험기간동안 증체량은 혼합조성물 10% 첨가군(B)을 제외하고, 혼합조성물의 첨가 수준이 높을수록 감소 경향을 나타내었다. 식이섭취량은 차이가 없이 혼합조성물의 첨가 수준이 높을수록 다소 증가한 반면, 식이효율은 혼합조성물의 첨가 수준이 높을수록 다소 감소하였다. 내장지방 중 신장주변지방패드(RFP) 무게는 고지방식이대조군(F)보다 혼합조성물 30% 첨가군(E)이 통계적으로 낮았고, 정소상체지방패드(EPF) 무게는 F군보다 혼합조성물 20% 첨가군(D)이 유의하게 낮았다(p<0.05). 배변량의 경우, 실험 전기간(10주) 고지방식이대조군(F)은 실험1기와 실험2기의 배변량이 차이가 없었고, 혼합조성물의 경우 첨가 수준이 높을수록 F군보다 통계적으로 유의하게 증가하였다(p<0.05). 소장길이는 실험군 간에 유의한 차이가 없이 혼합조성물 10%, 15% 및 20% 첨가군(B, C, D)이 F군보다 다소 긴 것으로 나타났고, 대장길이는 F군과 20% 첨가군(D) 간에 통계적으로 유의한 차이가 있었다(p<0.05). 혈청지질중 TC, HDL- 및 LDL-콜레스테롤 농도는 유의적인 차이가 없었고, TG 농도는 혼합조성물 10%(B)와 15% 첨가군(C)이 낮았고, F군과 다른 첨가군은 높았다. 혈당농도는 혼합조성물첨가군이 F군보다 통계적으로 유의하게 낮았다(p<0.05). 혈구세포(CBC)중 RBC와 WBC는 혼합조성물 30% 첨가군(E)이 F군보다 통계적으로 높았고, Hct와 Hb 함량은 E군이 F군보다 높았으며, 혈소판(Platelet) 수는 혼합조성물첨가군(A~E)이 F군보다 통계적으로 유의하게 높았다(p<0.05). 이상에서 고지방식이를 급여한 흰쥐에게 늙은 호박, 율무, 꾸지뽕잎 분말조성물첨가식이를 급여했을 때 체지방량 저하, 배변량 증가, 혈청중 TG 감소 및 혈당치의 유의한 감소작용이 있는 것으로 나타나서, 앞으로 이들 혼합조성물의 적절한 처방으로 체지방 억제, 배변활성 및 지질개선을 위한 소재화 기술개발이 필요한 것으로 판단된다.

This study investigated the effects of the diet with Cucurbita moschata (CM), adlay seed (AS), and Cudrania tricuspidata leaf (CTL) mixed-powder on the visceral fat, fecal amount, and serum lipid profiles in rats fed a high-fat diet (HFD). The mixed ratio of CM, AS, and CTL as a functional food ingredient was 1:1:1 on a weight basis. Male Sprague-Dawley rats were fed a high-fat diet (D12492; 45 kcal fat) for 5 weeks during the first phase. In the second phase, which lasted 5 weeks, the rats were divided into six experimental groups. The groups were: a high-fat diet group as a control during the 10 weeks of experimental period; and a high-fat diet with 5, 10, 15, 20, and 30% mixed-powder supplement group. The adipose tissue (RFP, EFP) weights were significantly decreased in the 20% and 30% mixed-powder supplement groups than in the HFD group (p<0.05). The fecal weight produced by the 15%, 20%, and 30% mixed-powder supplement groups was significantly increased during the second phase compared to the first phase (p<0.05). In terms of serum lipids, TG (triglyceride) content was decreased in the 10% and 15% mixed-powder supplement groups. The blood glucose levels were significantly decreased in all experimental diet supplement groups than in the HFD group (p<0.05). The findings show that the high supplement groups could improve the visceral fat accumulation, fecal amount, and blood glucose content compared to the HFD group.

키워드

참고문헌

  1. Anderson JW, Deakins DA, Floore TL, Smith BM, Whitis SE. 1990. Dietary fiber and coronary heart disease. Crit Rev Food Sci Nutr 29:95-147 https://doi.org/10.1080/10408399009527518
  2. Asano N, Oseki K, Tomioka E, Kizu H, Matsui K. 1994. N-containing sugars from Morus alba and their glycosidase inhibitory activities. Carbohyr Res 259: 243-255 https://doi.org/10.1016/0008-6215(94)84060-1
  3. Avram AS, Avram MM, James WD. 2005. Subcutaneous fat in normal and diseased status: 2. Anatomy and physiology of white and brown adipose tissue. J Am Acad Dermatol 53: 671-683 https://doi.org/10.1016/j.jaad.2005.05.015
  4. Burton GW, Ingold GW. 1984. An unusual type of lipid antioxidant. Science 224:56-63 https://doi.org/10.1126/science.224.4644.56
  5. Cha JY, Kim HJ, Jun BS, Cho YS. 2000. Effect of water-extracted of leaves from Morus alba and Cudrania tricuspidata on the lipid concentrations of serum and liver in rats. J Korean Soc Appl Biol Chem 43:303-308
  6. Chang CH, Lin CC, Hattori M, Namba T. 1994. Effects of antilipid peroxidation of Cudrania cochinchinensis var. gerontogea. J Ethnopharomacol 44:179-185
  7. Chung BS, Suzuki H, Hayakawa S, Kim JH, Nishizaawa Y. 1988. Studied on the plasma cholesterol-lowing component in coix. J Japan Food Technol 35:618-623 https://doi.org/10.3136/nskkk1962.35.9_618
  8. Chung SH, Yu JH, Kim EJ, Ryu KS. 1996. Blood glucose lowering effect of silkworm. Bull K H Pharma Sci 24: 95-100
  9. Fujimoto T, Nomura T. 1985. Compnents of root bark of Cudrania tricuspidata 3. Isolation and structure studies on the flavonoids. Planta Medica 51:190-196 https://doi.org/10.1055/s-2007-969453
  10. Han CK, Kim SS, Choi SY, Park JH, Lee BH. 2009. Effects of rice added with mulberry leaves and fruit on blood glucose, body fat and serum lipid levels in rats. J Korean Soc Food Sci Nutr 38:1336-1341 https://doi.org/10.3746/jkfn.2009.38.10.1336
  11. Jang JY, Choi HY. 2003. Effects of Artemisia iwayomogi oligosaccharide on the blood lipids, abdominal adipose tissues and leptin levels in the obese rats. Korean J Nutr 36:437-445
  12. Jang MJ, Rhee SJ. 2004. Hypoglycemic effects of pills made of mulberry leaves and silkworm powder in streptozotocininduced diabetic rats. J Korean Soc Food Sci Nutr 33: 1611-1617 https://doi.org/10.3746/jkfn.2004.33.10.1611
  13. Jin KD. 1975. Studies on coix ma-yuen roman. J Kor Pharm Sci 5:307-311
  14. Joo HY, Lim KT. 2009. Protective effect of glycoprotein isolated from Cudrania tricuspidata on liver in $CCl_4$-treated A/J mice. Korean J Food Sci Technol 41:93-99
  15. Kim HK, Cho DW, Hahm YT. 2000. The effects of coix bran on lipid metabolism and glucose challenge in hyperlipidemic and diabetic rats. J Korean Soc Food Sci Nutr 29:140-146
  16. Kim SH, Kim NJ, Choi JS, Park JC. 1993. Determination of flavonoid by HPLC and biological activities from the leaves of Cudrania tricuspidata Bureau. J Korean Soc Food Nutr 22:68-72
  17. Kim SY, Lee WC, Kim HB, Kim AJ, Kim SK. 1998. Antihyperlipidemic effects of methanol extracts from mulberry leaves in cholesterol induced hyperlipidemia in rats. J Korean Soc Food Sci Nutr 27:1217-1222
  18. Kritchevsky D. 1976. Diet and atherosclerosis. Am J Pathol 84: 615-632
  19. Kwon EH, Jung MA, Rhee SJ, Choi SW, Cho SH. 2006. Antioxidant effects of improvement of lipid metabolism of mulberry fruit, mulberry leaves and silkworm powder with different mixing ratios in streptozotocin-induced diabetic rats. Korean J Nutr 39:91-99
  20. Lee JE, Suh MH, Lee HG, Yang CB. 2002. Characteristics of Job's tear gruel by various mixing ratio, particle size and soaking time of Job's tear and rice flour. Korean J Soc Food Cookery Sci 18:193-199
  21. Lee JM, Lee SH, Lee JH, Lee E. 2006. Effects of Coix lachrymajobi var. mayuen Stapf. pharmacopuncture on plasma lipid composition and glucose in rat fed high fat diet. Kor J Acupuncture 23:59-66
  22. Lee JS, Lee MK, Ha TY, Bok SH, Park HM, Jeong KS, Woo MN, Do GM, Yeo JY, Choi MS. 2006. Supplementation of whole persimmon leaf improves lipid profiles and suppresses body weight gain in rats fed high-fat diet. Food Chem Toxicol 44:1875-1883 https://doi.org/10.1016/j.fct.2006.06.014
  23. Lee KS, Hwang CS. 1990. A study on the actual utilization korean traditional remedies-About foods used on geriatric disease. Korean J Dietary Culture 5:331-347
  24. Lee S, So S, Hwang E, Koo B, Han G, Ko S, Kim N. 2008. Effect of ginseng and herbal plant mixtures on anti-obesity in obese SD rat induced by high fat diet. J Korean Soc Food Sci Nutr 37:437-444 https://doi.org/10.3746/jkfn.2008.37.4.437
  25. Lee SW. 1992. Research on Ancient Korea Dietary Life History in the East Asia. Hyangmoonsa, Seoul, Korea. p 122
  26. Lim SC. 2006. Effects of yullmoo (Coix lachryma-jobi var. mayuen Stapf.) ext. on lipid lowering and serum glucose in hyperlipidemic rat. Korean J Plant Res 19:126-129
  27. McDougall GJ, Morrison IM, Hillman JR. 1996. Plant cell walls dietary fibre: range, structure, processing and function. J Sci Food Agric 70:133-150 https://doi.org/10.1002/(SICI)1097-0010(199602)70:2<133::AID-JSFA495>3.0.CO;2-4
  28. Ministry of Health and Welfare. 2005. Year Book of Health and Welfare Statistics. Korea. pp 51-77
  29. Oh UJ, Kim GP, Cho YW, Chung SH, Gu SJ. 1999. Effect of beverage containing extract from mulberry leaves on serum glucose and lipid levels in db/db mouse. Annual Meeting of Korean Soc Food Sci Technol, Seoul, Korea. p 430
  30. Otlersen T, Vance B, Doorenbos NJ, Chang BL, EI-Feraly FS. 1977. The crystal structure of cudranone, 2,6,3'-trihydroxy- 4-methoxy-2'-(3-methoxy-2-buternyl)-I, a new a-ntimicrobial agent from Cudrania cochinchinensis. Acta Chem Scand 31: 434-436
  31. Park IC, Young HS, Choi JS. 1992. Constituents of Cudrania tricuspidata in Korea. Yakhak Hoeji 36:40-45
  32. Park YH, Lee JS, Lee YJ. 1993. Distribution of serum lipid by age and the relation of serum lipid to degree of obesity and blood pressure in Korean adults. Kor J Lipidiology 3: 165-180
  33. Park YJ, Lee YS. 1988. Effect of coix on plasma cholesterol and lipid metabolism in rats. Korean J Nutr 21:88-98
  34. Reeves PG, Nielsen FH, Fahey GC. 1993. AIN-93 purified diets for laboratory rodent: final report of the American Institute Nutrition ad hoc writing committee in the reformulation of the AIN-76A rodent diets. J Nutr 123:1939-1951 https://doi.org/10.1093/jn/123.11.1939

피인용 문헌

  1. Effects of Phytoplant Diets on Body Weight, Feces Production, Body Fat, and Serum Lipid Levels in High-fat Diet-induced Hyperlipidemic Rats vol.46, pp.4, 2014, https://doi.org/10.9721/KJFST.2014.46.4.477
  2. Physiological Activities of Cudrania tricuspidata Extracts (Part I) vol.14, pp.8, 2013, https://doi.org/10.5762/KAIS.2013.14.8.3907
  3. Effects of the KI-180 and KI-188 Calcium Formulae on the Growth and Development of Rats vol.46, pp.3, 2014, https://doi.org/10.9721/KJFST.2014.46.3.358
  4. Antioxidant and Anti-Adipogenic Activities of Bread Containing Corn Silk, Job's Tears, Lentinus edodes, and Apple Peel in 3T3-L1 Preadipocytes vol.45, pp.5, 2016, https://doi.org/10.3746/jkfn.2016.45.5.651
  5. 꾸지뽕나무 추출물의 피부 생리 활성 vol.32, pp.2, 2012, https://doi.org/10.12925/jkocs.2015.32.2.260
  6. The Effect of Yulmu (Coix lacryma-jobi L.)-Sunsik on Obesity and Hyperlipidemia in Mice Fed High-Fat Diet vol.50, pp.7, 2021, https://doi.org/10.3746/jkfn.2021.50.7.664