The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Immune Enhancement of Hericium erinaceum Mycelium Cultured in Submerged Medium Supplemented with Ginseng Extract

수삼 추출물 첨가 액체배지에서 배양된 노루궁뎅이버섯 균사체의 면역활성 증진

  • Kim, Hoon (R & D Center, CosisBio Corporation Limited) ;
  • Ra, Kyung-Soo (Division of Food Beverage and Culinary Arts, Daegu Technical University) ;
  • Hwang, Jong-Hyun (Dept. of Food and Nutrition, Korea National University of Transportation) ;
  • Yu, Kwang-Won (Dept. of Food and Nutrition, Korea National University of Transportation)
  • 김훈 ((주)코시스바이오 기업부설연구소) ;
  • 나경수 (대구공업대학교 호텔외식조리계열) ;
  • 황종현 (한국교통대학교 식품영양학과) ;
  • 유광원 (한국교통대학교 식품영양학과)
  • Received : 2012.09.20
  • Accepted : 2012.10.11
  • Published : 2012.12.31
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Abstract

본 연구는 mushroom complete medium(MCM) 액체배지에 수삼 추출물(GE, $65^{\circ}Bx$)을 첨가하여 면역활성이 증진된 노루궁뎅이버섯(Hericium erinaceum) 균사체를 배양하고, 균사체로부터 활성다당성분을 분획하고자 하였다. MCM에 대하여 GE를 5, 10과 15%(v/v) 첨가한 액체배지에서 균사체를 배양하고, 각각의 조다당획분(HE-GE-5-CP, HE-GE-10-CP와 HE-GE-15-CP)으로 분획하여 면역활성을 측정한 결과, HE-GE-10-CP는 HE-GE-5-CP와 HE-GE-15-CP보다 높은 활성을 나타내었으며, GE를 첨가하지 않은 MCM에서 배양된 균사체 조다당획분(HE-CP)보다 유의적으로 증진된 면역활성을 나타내었다. 또한, HE-GE-10-CP의 DEAE-Sepharose CL-6B 분획물 중 가장 높은 활성을 나타낸 HE-GE-10-CP-II획분은 대조군인 HE-CP의 어떠한 획분보다도 유의적으로 높은 면역활성과 암 전이 억제활성을 나타내었다. 한편, 활성획분인 HE-GE-10-CP-II는 arabinose, rhamnose, galactose, glucose와 uronic acid(molar ratio; 0.34:0.26:0.99:1.00:0.39)로 구성되어 있으나, 대조군인 HE-CP의 동일용매 용출획분으로서 HE-GE-10-CP-II보다는 활성이 낮은 HE-CP-II는 fucose, mannose, galactose와 glucose(molar ratio; 0.32:0.55:1.00:0.96)를 함유하여 다른 구성당 분포를 나타내었다. 따라서 노루궁뎅이버섯 균사체 액체배양에서 수삼 추출물 첨가는 균사체의 구성당 변화를 통한 면역활성 증진에 관여하는 것으로 사료되어 기능성 소재 개발에 유용할 것으로 사료된다.

Keywords

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