Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Apoptosis Induction, Cell Cycle Arrest and in Vitro Anticancer Activity of Gonothalamin in a Cancer Cell Lines

  • Alabsi, Aied M. (Department of Biotechnology, Universiti Sultan Zainal Abidin (UniSZA)) ;
  • Ali, Rola (Department of Biotechnology, Universiti Sultan Zainal Abidin (UniSZA)) ;
  • Ali, Abdul Manaf (Department of Biotechnology, Universiti Sultan Zainal Abidin (UniSZA)) ;
  • Al-Dubai, Sami Abdo Radman (Community Medicine Department, International Medical University) ;
  • Harun, Hazlan (Department of Chemistry, Universiti Sultan Zainal Abidin (UniSZA)) ;
  • Kasim, Noor H. Abu (Faculty of Dentistry, Universiti of Malaya) ;
  • Alsalahi, Abdulsamad (Faculty of Pharmacy, Sana'a University)
  • Published : 2012.10.31
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Abstract

Cancer is one of the major health problems worldwide and its current treatments have a number of undesired adverse side effects. Natural compounds may reduce these. Currently, a few plant products are being used to treat cancer. In this study, goniothalamin, a natural occurring styryl-lactone extracted from Goniothalamus macrophyllus, was investigated for cytotoxic properties against cervical cancer (HeLa), breast carcinoma (MCF-7) and colon cancer (HT29) cells as well as normal mouse fibroblast (3T3) using MTT assay. Fluorescence microscopy showed that GTN is able to induce apoptosis in HeLa cells in a time dependent manner. Flow cytometry further revealed HeLa cells treated with GTN to be arrested in the S phase. Phosphatidyl serine properties present during apoptosis enable early detection of the apoptosis in the cells. Using annexin V/PI double staining it could be shown that GTN induces early apoptosis on HeLa cells after 24, 48 and 72 h. It could be concluded that goniothalamin showing a promising cytotoxicity effect against several cancer cell lines including cervical cancer cells (HeLa) with apoptosis as the mode of cell death induced on HeLa cells by Goniothalamin was.

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References

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