The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Occurrence of Stem Canker and Tuber Rot on Yam Caused by Rhizoctonia solani AG 2-2IIIB in Korea

Rhizoctonia solani AG 2-2IIIB에 의한 마 뿌리썩음병의 한국 내 발생

  • Hong, Sung Kee (Crop Protection Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Lee, Jae Kook (Crop Protection Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Lee, Young Kee (Crop Protection Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Lee, Sang Yeob (Agricultural Microbiology Division, NAAS, RDA) ;
  • Kim, Wan Gyu (Agricultural Microbiology Division, NAAS, RDA) ;
  • Shim, Hong Sik (Crop Protection Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA))
  • 홍성기 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 이재국 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 이영기 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 이상엽 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 김완규 (농촌진흥청 국립농업과학원 농업미생물과) ;
  • 심홍식 (농촌진흥청 국립농업과학원 작물보호과)
  • Received : 2012.11.12
  • Accepted : 2012.12.03
  • Published : 2012.12.31
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Abstract

Stem canker and tuber rot symptoms were observed on yam grown in Andong and Jinju, Korea in 2011. A total of 20 isolates of Rhizoctonia and allied fungi were obtained from the symptomatic plants. Among the isolates, 8 isolates were identified as Rhizoctonia solani and 12 isolates as Ceratobasidium sp. based on rDNA-internal transcribed spacer (ITS) sequence similarity. In the cluster analysis of rDNA-ITS sequences, 7 isolates of R. solani belonged to AG 2-2IIIB and remaining one to AG 1-1A. In addition, among the 12 isolates of Ceratobasidium sp., 7 isolates belonged to AG-Fa, three isolates to AG-A and the other two isolates to AG-Fb and AG-O, respectively. Pathogenicity tests showed that all the R. solani AG 2-2IIIB isolates are pathogenic on stem and tuber of yam but R. solani AG 1-1A and all the Ceratobasidium isolates are non-pathogenic. The results indicate that R. solani AG 2-2IIIB is an important pathogen causing stem canker and tuber rot on yams grown in the study areas. This is the first report of R. solani AG 2-2IIIB causing stem canker and tuber rot of yam in Korea.

2011년 안동과 진주의 마 재배포장에서 줄기 지제부 및 괴근썩음 증상이 나타났다. 병징을 나타내는 부위로부터 Rhizoctonia와 유사 속에 속하는 20개 균주가 분리되었다. rDNA-internal transcribed spacer(ITS) 염기서열 상동성을 기초로 8균주는 Rhizoctonia solani, 12균주는 Ceratobasidium sp.로 동정되었다. rDNA-ITS 염기서열의 cluster 분석에 의해 R. solani에 속하는 8개 균주 중 7개 균주는 균사융합군 AG 2-2IIIB, 1균주는 AG 1-1A에 속하였다. 또한, Ceratobasidium sp.에 속하는 12균주 중 7균주는 AG-Fa, 3균주는 AG-A, 나머지 2균주는 각각 AG-Fb와 AG-O에 속하였다. R. solani AG 2-2IIIB 균주들은 마의 줄기와 괴근에 병원성이었으나 R. solani AG 1-1A와 모든 Ceratobasidium sp. 균주는 비병원성이라는 것이 확인되었다. 이 결과는 조사지역에서 R. solani AG 2-2IIIB가 마의 줄기 및 괴근썩음병을 일으키는 중요한 병원균이라는 것을 나타낸다. 이 연구는 국내에서 R. solani AG 2-2IIIB에 의한 마 뿌리썩음병에 대하여 처음으로 보고하는 것이다.

Keywords

References

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