The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Molecular Identification of Endophytic Fungi Isolated from Needle Leaves of Pinus thunbergii

곰솔(Pinus thunbergii)의 침엽에서 분리한 내생균의 분자적 동정

  • Kim, Chang-Kyun (Department of Biology Education, Korea National University of Education) ;
  • Eo, Ju-Kyeong (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 김창균 (한국교원대학교 생물교육과) ;
  • 어주경 (한국교원대학교 생물교육과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2012.11.16
  • Accepted : 2012.11.27
  • Published : 2012.12.31
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Abstract

Endophytic fungi were isolated from needle leaves of Pinus thunbergii distributed in Boryeong of Korea. Total 7 species of fungi were identified using partial sequences of ITS and LSU regions of nuclear DNA Anthostomella sepelibilis, Beltrania sp., Cladosporium sp., Coniochaeta velutina, Entonaema pallida, Lophodermium sp. and Nigrospora sphaerica sphaerica. The endophytic fungi, Beltrania sp. was the most dominant species isolated from P. thunbergii in this study. Results indicate that the distribution of endophytic fungi in the leaves of P. thunbergii could be influenced by environments of the fungal habitat.

곰솔(Pinus thunbergii Parl.)의 잎에 서식하는 내생균의 다양성을 확인하기 위하여 충남 보령의 해안가에 서식하는 곰솔에서 잎을 채취하여 총 19개의 균주를 분리하였다. 분리된 내생균을 ITS 지역과 LSU 지역의 염기서열을 분석하여 동정한 결과 Anthostomella sepelibilis, Beltrania sp., Cladosporium sp., Coniochaeta velutina, Entonaema pallida, Lophodermium sp., Nigrospora sphaerica 등 총 7개 분류군의 내생균을 확보할 수 있었다. 이들 중 Beltrania sp.에 속하는 종이 가장 높은 빈도로 분리되었으며 내생균의 서식 환경이 곰솔의 잎에 서식하는 내생균의 분포에 영향을 미칠 수 있음을 확인하였다.

Keywords

References

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