Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effect of Bcl-2 Inhibitor Treatment on Embryo Developmental Competence, Apoptosis and ER-stress in Pigs

Bcl-2의 저해제 처리에 따른 돼지 수정란의 배발달 능력, 세포 사멸 및 소포체 스트레스 양상

  • Hong, Joo-Hee (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Min, Sung-Hun (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Lee, Enok (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Son, Hyeong-Hoon (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Yeon, Ji-Yeong (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Park, Humdai (Department of Biotechnology, College of Engineering, Daegu University) ;
  • Koo, Deog-Bon (Department of Biotechnology, College of Engineering, Daegu University)
  • 홍주희 (대구대학교 공과대학 생명공학과) ;
  • 민성훈 (대구대학교 공과대학 생명공학과) ;
  • 이에녹 (대구대학교 공과대학 생명공학과) ;
  • 손형훈 (대구대학교 공과대학 생명공학과) ;
  • 연지영 (대구대학교 공과대학 생명공학과) ;
  • 박흠대 (대구대학교 공과대학 생명공학과) ;
  • 구덕본 (대구대학교 공과대학 생명공학과)
  • Received : 2012.08.28
  • Accepted : 2012.09.17
  • Published : 2012.09.30
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Abstract

The key regulators of apoptosis are the interacting protein of the Bcl-2 family. Bcl-2, an important member of this family, blocks cytochrome C release by sequestering pro-apoptotic BH3-only proteins such as Bid, Bad, Bax and Bim. The pro-survival family members (Bcl-2, Bcl-XL, Bcl-W) are critical for cell survival, since loss of any of them causes cell death in certain cell type. However, its role during early porcine embryonic development is not sufficient. In this study, we traced the effects of Bcl-2 inhibitor, ABT-737, on early porcine embryonic development. We also investigated several indicators of developmental potential, including gene expression (apoptosis-related genes) and apoptosis, which are affected by ABT-737. Porcine embryos were cultured in the PZM-3 medium with or without ABT-737 for 6 days. In result, significant differences in developmental potential were detected between the embryos that were cultured with or without ABT-737 ($14.7{\pm}3.0$ vs $30.3{\pm}4.8%$, p<0.05). TUNEL assay showed that the number of containing fragmented DNA at the blastocyst stage increased in the ABT-737 treated group compared with control (4.7 vs 3.7, p<0.05). The mRNA expression of the pro-apoptotic gene Bax increased in ABT-737 treated group (p<0.05), whereas expressions of the anti-apoptotic Bcl-2 family members (Bcl-2, Bcl-XL, Bcl-W) decreased (p<0.05). Also, expressions of the ER stress indicator genes (GRP78, XBP-1 and sXBP-1) increased in ABT-737 treated group (p<0.05). In conclusion, Bcl-2 is closely associated with of apoptosis- and ER stress-related genes expressions and developmental potential in pig embryos.

Keywords

References

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