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Carbohydrase inhibition and anti-cancerous and free radical scavenging properties along with DNA and protein protection ability of methanolic root extracts of Rumex crispus

  • Shiwani, Supriya (Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University) ;
  • Singh, Naresh Kumar (Department of Animal Biotechnology, College of Animal Life Sciences, Kangwon National University) ;
  • Wang, Myeong Hyeon (Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University)
  • 투고 : 2012.05.01
  • 심사 : 2012.08.08
  • 발행 : 2012.10.31

초록

The study elucidated carbohydrase inhibition, anti-cancerous, free radical scavenging properties and also investigated the DNA and protein protection abilities of methanolic root extract of Rumex crispus (RERC). For this purpose, pulverized roots of Rumex crispus was extracted in methanol (80% and absolute conc.) for 3 hrs for $60^{\circ}C$ and filtered and evaporated with vacuum rotary evaporator. RERC showed high phenolic content ($211{\mu}g$/GAE equivalent) and strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ($IC_{50}$ = 42.86 (absolute methanol) and $36.91{\mu}g/mL$ (80% methanolic extract)) and reduced power ability. Furthermore, RERC exhibited significant protective ability in $H_2O_2/Fe^{3+}$/ascorbic acid-induced protein or DNA damage and percentage inhibition of the HT-29 cell growth rate following 80% methanolic RERC exposure at $400{\mu}g/mL$ was observed to be highest ($10.2%{\pm}1.03$). Moreover, methanolic RERC inhibited ${\alpha}$-glucosidase and amylase effectively and significantly (P < 0.05). Conclusively, RERC could be considered as potent carbohydrase inhibitor, anti-cancerous and anti-oxidant.

키워드

참고문헌

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