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Transmembrane Protein 166 Expression in Esophageal Squamous Cell Carcinoma in Xinjiang, China

  • Sun, Wei (Department of Thoracic Oncology, the Affiliated Tumor Hospital of Xinjiang Medical University) ;
  • Ma, Xiu-Min (State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, the First Affiliated Hospital of Xinjiang Medical University) ;
  • Bai, Jing-Ping (Department of Thoracic Oncology, the Affiliated Tumor Hospital of Xinjiang Medical University) ;
  • Zhang, Guo-Qing (Department of Thoracic Oncology, the Affiliated Tumor Hospital of Xinjiang Medical University) ;
  • Zhu, Yue-Jie (National Clinical Research Base of Traditional Chinese Medicine of Xinjiang Medical University) ;
  • Ma, Hai-Mei (State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, the First Affiliated Hospital of Xinjiang Medical University) ;
  • Guo, Hui (National Clinical Research Base of Traditional Chinese Medicine of Xinjiang Medical University) ;
  • Chen, Ying-Yu (Laboratory of Medical Immunology, School of Basic Medical Science, Peking University Health Science Center) ;
  • Ding, Jian-Bing (National Clinical Research Base of Traditional Chinese Medicine of Xinjiang Medical University)
  • Published : 2012.08.31

Abstract

Objective: Transmembrane protein 166 (TMEM166) expression in esophageal squamous cell carcinoma (ESCC) and remote normal esophageal tissues was examined to assess any role in tumour biology. Methods: TMEM166 mRNA expression in 36 cases with ESCC (36 tumour samples, 36 remote normal esophageal tissue samples) was detected by RT-PCR. TMEM166 protein expression was analysed in paraffin-embedded tissue samples from the same cases by immunohistochemistry. Results: Semi-quantitative analysis showed TMEM166 mRNA expression in ESCCs to be significantly lower than in remote normal esophageal tissues ($0.759{\pm}0.713$ vs. $2.622{\pm}1.690$, P=0.014). TMEM166 protein expression was also significantly reduced (69.4% vs. 94.4%, P<0.01). Conclusion: TMEM166 mRNA and protein expression demonstrated significant reduction in ESCCs compared with remote esophageal tissues, albeit with no correlation with tumour size, differentiation, stage, and lymph node metastasis, suggesting a role in regulating autophagic and apoptotic processes in the ESCC.

Keywords

References

  1. Arico S, Petiot A, Bauvy C, et al (2001). The tumor suppressor PTEN positively regulates macroautophagy by inhibiting the phosphatidylinositol 3-kinase/protein kinase B pathway. J Biol Chem, 276, 3524-46. https://doi.org/10.1074/jbc.M004275200
  2. Boya P, Gonzalez-Polo RA, Casares N, et al (2005). Inhibition of macroautophagy triggers apoptosis. Mol Cell Biol, 25, 1025-40. https://doi.org/10.1128/MCB.25.3.1025-1040.2005
  3. Fang Y, Xiao F, An Z, et al (2011). Systematic review on the relationship between genetic polymorphisms of methylenetetrahydrofolate reductase and esophageal squamous cell carcinoma. Asian Pac J Cancer Prev, 12, 1861-6.
  4. Gozuacik D, Kimchi A (2004). Autophagy as a cell death and tumor suppressor mechanism. Oncogene, 23, 2891-906. https://doi.org/10.1038/sj.onc.1207521
  5. Guo B, Huang Z L(2011). Esophageal cancer mortality trends in rural and urban China between 1987 and 2009. Asian Pac J Cancer Prev, 12, 2105-10.
  6. Inbal B, Bialik S, Sabanay I, et al (2002). DAP kinase and DRP-1 mediate membrane blebbing and the formation of autophagic vesicles during programmed cell death. J Cell Biol, 157, 455-68. https://doi.org/10.1083/jcb.200109094
  7. Klionsky DJ, Emr SD (2000). Autophagy as a regulated pathway of cellular degradation. Science, 290, 1717-21. https://doi.org/10.1126/science.290.5497.1717
  8. Lagergren J, Bergstrom R, Nyren O (1999). Association between body mass and adenocarcinoma of the esophagus and gastric cardia. Ann Intern Med, 130, 883-90. https://doi.org/10.7326/0003-4819-130-11-199906010-00003
  9. Wang L, Yu CF, Lu Y, et al (2007). TMEM166, a novel transmembrane protein, regulates cell autophagy and apoptosis. Apoptosis, 12, 1489-502. https://doi.org/10.1007/s10495-007-0073-9
  10. Levine B, Yuan J (2005). Autophagy in cell death: an innocent convict? J Clin Invest, 115, 2679 -88. https://doi.org/10.1172/JCI26390
  11. Li L, Nikan H, Qin H, et al (2012). Transmembrane protein 166 regulates autophagic and apoptotic activities following focal cerebral ischemic injury in rats. Exp Neurol, 234, 181-90. https://doi.org/10.1016/j.expneurol.2011.12.038
  12. Liu JF, Wang QZ, Hou J (2004 ) . Surgical treatment for cancer of the esophagus and gastric cardia in Hebei. China Br J Surg, 91, 90-8. https://doi.org/10.1002/bjs.4402
  13. Lum JJ, DeBerardinis R, Thompson CB (2005). Autophagy in metazoans, cell survival in the land of plenty. Nat Rev Mol Cell Biol, 6, 439-48 https://doi.org/10.1038/nrm1660
  14. Mizushima N (2005). The pleiotropic role of autophagy: from protein metabolism to bactericide. Cell Death Differ, 12, 1535-41 https://doi.org/10.1038/sj.cdd.4401728
  15. Parkin DM, Bray F, Ferlay J, Pisani P (2005). Global cancer statistics, 2002. CA Cancer JClin, 55, 74-108. https://doi.org/10.3322/canjclin.55.2.74
  16. Shohat G, Shani G, Eisenstein M, Kimchi A (2002). The DAP kinase family of proteins: study of a novel group of calciumregulated death-promoting kinases. Biochim Biophys Acta, 1600, 45-50. https://doi.org/10.1016/S1570-9639(02)00443-0
  17. Sun X, Chen W, Chen Z, et al (2010). Population-based casecontrol study on risk factors for esophageal cancer in five high-risk areas in China. Asian Pac J Cancer Prev, 11, 1631-6.
  18. Tomoyuki A, Masahiko I, Hiroshi I, et al (2009). Macroscopic extent of gastric mucosal atrophy: increased risk factor for esophageal squamous cell carcinoma in Japan. BMC Gastroenterology, 9, 34-9. https://doi.org/10.1186/1471-230X-9-34
  19. Yamada KM, Araki M (2001). Tumor suppressor PTEN: modulator of cell signaling, growth, migration and apoptosis. J Cell Sci, 114, 2375-82.
  20. Yu GZ, Zhu MH, Chen Y, Ni CR, Li FM (2005). Clinical significance of p53 expression in pancreatic cancer. Cancer, 24, 1398-403.

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