Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
권호 표지
DOI QR코드

DOI QR Code

Species Identification and Developmental Biology of a Red Bean Pest in Ostrinia sp. (Lepidoptera: Crambidae)

Ostrinia속(나비목: 포충나방과) 팥 해충의 종 동정과 발육 특성

  • Received : 2012.09.25
  • Accepted : 2012.11.11
  • Published : 2012.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Ostrinia larvae feed the pods and stem of red bean and seriously damage the bean production from farmers. In this study we investigated biological and developmental characteristics including field collection, host feeding preference, artificial rearing diet, morphological and molecular taxonomical identification, and pheromone analysis for an Ostrinia sp. in Korea. The male adults have massive tibia in the middle legs and 3-lobed uncus in the genitalia. The partial nucleotide sequences of mitochondrial cytochrome oxidase I (COI) and II (COII) were not corresponded to those DNA sequences from other Ostrinia species reported previously in Japan and China. Host plants for this species are also different from the previous species reported. In the gas chromatography (GC) analyses, (Z)-9-tetradecenyl acetate was not detected from the pheromone gland of our species while the component as a sex pheromone was found in O. zaguliaevi and O. zealis, With taken results, we conclude this Ostrinia species in Korea is Ostrinia scapulalis or closely related species. When larvae collected in a fall were incubated in the outdoor condition, they emerged to adult between June and July in the next year. The result indicates that the winter diapause could be started in late larval stage. In addition, we developed a semi-synthetic artificial diet adopted for mass rearing of the O. scapulalis in laboratory.

본 연구에서는 팥의 꼬투리와 줄기를 가해하는 Ostrinia속 해충에 대해 종 동정 과정을 기술하였고, 사육하면서 관찰된 발육특성들을 보고하였다. 수컷의 생식기는 3-lobed uncus 형태였으며, 가운뎃다리 종아리마디에는 털을 많이 갖는 것으로 나타났다. 미토콘드리아 cytochrome oxidase I (COI)과 II (COII) 유전자의 부분 염기서열은 콩줄기명나방(O. scapulalis), 큰섬들명나방(O. zaguliaevi), 큰조명나방(O. zealis bipatrialis)들에 대해 일본과 중국에서 보고된 서열들과 100% 일치를 보이는 종은 없었다. 기주식물 범위는 국내외 보고들 간에 일치하지 않았다. 암컷 성페로몬샘 추출물의 가스크로마토그래피 분석에서 큰섬들명나방과 큰조명나방의 성페로몬 성분인 (Z)-9-tetradecenyl acetate는 검출되지 않았다. 이상의 결과들을 종합하여 고려하였을 때, 본 연구의 팥 해충을 가해하는 곤충 종은 콩줄기명나방(O. scapulalis)일 것으로 추정되었다. 가을 야외에서 채집된 유충들을 야외조건에서 보관하였을 때, 이듬해 6월과 7월 사이에 성충들이 우화하였는데, 이 결과로부터 본 곤충 종은 말령 유충 단계에서 겨울휴면을 하는 것으로 추정되었다. 이외에 반합성 인공사료를 이용하여 실내 사육이 가능하였다.

Keywords

References

  1. Bae, Y.S., 2001. Family Pyraloidea: Pyraustinae & Pyraliae. Economic Insects of Korea 9. Ins. Koreana Suppl. 16, 252p. Junghaeng-Sa, Seoul.
  2. Frolov, A.N., Bourguet, D., Ponsard, S., 2007. Reconsidering the taxonomy of several Ostrina species in the light of reproductive isolation: a tale for Ernst Mayr. Biol. J. Linnean Soc. 91, 49-72. https://doi.org/10.1111/j.1095-8312.2007.00779.x
  3. Frolov, A.N., Audiot P.. Bourguet, D., Kononchuk, A.G., Malysh, J.M., Ponsard, S., Streiff, R., Tokarev, Y.S., 2012. From Russia with lobe: genetic differentiation in trilobed uncus Ostrinia spp. follows food plant, not hairy legs. Heredity 108, 147-156. https://doi.org/10.1038/hdy.2011.58
  4. Goto, M., Sekine, Y., Outa, H., Hujikura, M., Suzuki, K., 2001. Relationships between cold hardiness and diapauses, and between glycerol and free amino acid contents in overwintering larvae of the oriental corn borer, Ostrinia furnacalis. J. Insect Physiol. 47, 157-165. https://doi.org/10.1016/S0022-1910(00)00099-8
  5. Hajibabaei M., Janzen, D.H., Burns, J.M., Hallwachs, W., Hebert, P.D., 2006. DNA barcodes distinguish species of tropical Lepidoptera. Proc. Natl. Acad. Sci. 103, 968-971. https://doi.org/10.1073/pnas.0510466103
  6. Hattori, I., Mutuura, A., 1987. Identification of Japanese species belonging to the genus Ostrinia with the host relationship. Plant Protect. 41, 24-31.
  7. Huang, Y., Tatsuki, S., Kim, C.-G., Hoshizaki, S., Yoshiyasu, Y., Honda H., Ishikawa, Y., 1997. Identification of sex pheromone of adzuki bean borer, Ostrinia scapulalis. J. Chem. Ecol. 23, 2791-2802. https://doi.org/10.1023/A:1022567111508
  8. Huang, Y., Honda, H., Yoshiyasu, Y., Hoshizaki, S., Tatsuki, S., Ishikawa, Y,. 1998. Sex pheromone of the butterbur borer, Ostrinia zaguliaevi. Entomol. Exp. Appl. 89, 281-287. https://doi.org/10.1046/j.1570-7458.1998.00410.x
  9. Huang, Y., Takanashi, T., Hoshizaki, S., Tatsuki, S., Ishikawa, Y., 2002. Female sex pheromone polymorphism in adzuki bean borer, Ostrinia scapulalis, is similar to that in European corn borer, O. nubilalis. J. Chem. Ecol. 28, 533-539. https://doi.org/10.1023/A:1014540011854
  10. Ishikawa, Y., Takanishi, T., Huang, Y., 1999a. Comparative studies on the sex pheromones of Ostrinia spp. in Japan: the burdock borer, Ostrinia zealis. Chemoecology 9, 25-32. https://doi.org/10.1007/s000490050030
  11. Ishikawa, Y., Takanashi, T. Kim, C., Hoshizaki, S., Tatsuki, S., Huang, Y., 1999b. Ostrinia spp. in Japan: their host plants and sex pheromones. Entomol. Exp. Appl. 91, 237-244. https://doi.org/10.1046/j.1570-7458.1999.00489.x
  12. Jung, J.K., Park, J.H., Im D.J., Han, T.M., 2005. Parasitism of Trichogramma evanescens and T. ostriniae (Hymenoptera: Trichogrammatidae) to eggs of the Asian corn borer, Ostrinia furnacalis (Lepidoptera: Pyralidae). Korean J. Appl. Entomol. 44, 43-50.
  13. Jung, J.K., Seo, B.Y., Park, J.H., Moon, J.K., Choi, B.S., Lee, Y.H., 2007. Developmental characteristics of soybean podworm, Matsumuraeses phaseoli (Lepidoptera: Tortricidae) and legume pod borer, Maruca vitrata (Lepidoptera: Pyralidae) on semi-synthetic artificial diets. Korean J. Appl. Entomol. 46, 393-399. https://doi.org/10.5656/KSAE.2007.46.3.393
  14. Jung, J.K., Seo, B.Y., Cho, J.R., Kwon, Y.H., Kim, G.H., 2009. Occurrence of lepidopteran insect pests and injury aspects in Adzuki bean fieds. Korean J. Appl. Entomol. 48, 29-35. https://doi.org/10.5656/KSAE.2009.48.1.029
  15. Kim, C., Hoshizaki, S., Huang, Y., Tatsuki, S., Ishikawa, Y., 1999. Usefulness of mitochondrial COII gene sequences in examining phylogenetic relationships in the Asian corn borer, Ostrinia furnacalis, and allied species (Lepidoptera: Pyralidae). Appl. Entomol. Zool. 34, 405-412.
  16. Mutuura, A., Munroe, E., 1970. Taxonomy and distribution of the European corn borer and allied species: Genus Ostrinia (Lepidoptera: Pyralidae). Memoirs of the Entomological Society of Canada, 102, 1-112.
  17. Park, K.T., 1975. Taxonomic study of the corn stem boror in Korea with allied species of the Genus Ostrinia(Lep.; Pyralidae). Kor. J. Pl. Prot. 14, 221-225.
  18. Park, K.T., 1983a. Pyralidae, in: Shin, Y.H., Park, K.T., Nam, S.H.(Eds.), Illustrated Flora & Fauna of Korea Vol. 27 Insecta(IX). 1053pp. Ministry of Education, Seoul. pp.374-376, Plate 24.
  19. Park, K.T., 1983b. Microlepidoptera of Korea. Insects Koreana, Ser. 3, 149-157.